Navigation Links
Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A


Introduction
High-resolution 2DE is the most widely used separation method for global proteome analysis. Continuous improvements in the technique are expanding the capabilities of gel separations and removing limits on the types of proteins that can be resolved. Here, we describe new sample preparation kits that enhance the detection capabilities of 2-D gels.

These protocols address two key issues in applying 2-D gels to proteomics analysis of complex samples. One issue is how to handle both the enormous chemical diversity and the wide dynamic range of proteins in a biological sample so that lowabundance and housekeeping proteins can be monitored and identified. We have developed a number of protocols to quickly prepare highly enriched protein fractions based on protein solubility. One protocol separates a complex sample into enriched fractions of nuclear and cytoplasmic proteins. A second protocol enriches for membrane-associated signaling proteins. Other protocols enrich for membrane proteins. All protocols enhance the capability to monitor and identify proteins of biological interest.

The other issue is how to solubilize these hydrophobic proteins so they can be separated in 2-D gels. A number of new detergents and more stringent chaotropic agents have been incorporated into a complete protein solubilization buffer (PSB), which efficiently solubilizes hydrophobic proteins for immediate application to a 2-D gel. Using both the simplified enrichment and enhanced solubilization protocols, we significantly expanded the capabilities of applying 2-D gels for proteome analysis of complex cellular extracts.


Methods
Sample Preparation
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Sample Preparation for Microinjection
2. GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA
3. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
4. A Better Way to Isolate RNA AND Protein From the Same Sample
5. Isolate Total RNA and Protein From the Same Sample
6. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
7. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
8. RNA from LCM Samples
9. RNA, DNA, and Protein from a Single Sample
10. Robust High Throughput RNA Isolation from Blood Samples
11. RNAlater Around the World: The Art of Processing Difficult Samples
Post Your Comments:
(Date:7/10/2014)... 2014 Product and process impurities ... abundance and are often “lost in the noise,” ... Join presenters Dr. Rowel Tobias, Senior Scientist, Protein ... Head of Quality at Nanotherapeutics, Inc., to lear ... speed detection and quantitation while achieving the required ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Terascala, the ... announced that Alan Swahn, a former vice president of ... team as vice president of marketing. Terascala’s software when ... and NetApp create the highest performance and most reliable ... appointed to guide Terascala’s channel expansion and broaden its ...
(Date:7/10/2014)... OMICS Group’s 5th International conference and exhibition ... at Double Tree by Hilton Beijing, China is all ... & Bioanalytical research methods only to facilitate improved living ... Dr. Srinubabu Gedela, MD of OMICS Group Inc has ... one in bringing a unique and international mix of ...
(Date:7/10/2014)... , July 10, 2014  Franciscan ... use of capnography for respiratory monitoring outside ... of healthcare leaders in embracing state-of-the-art patient ... effectively patients are breathing and can alert ... By measuring the amount of carbon dioxide ...
Breaking Biology Technology:Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Terascala Expands Executive Team 2Terascala Expands Executive Team 3A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2
... hours a day now that a new company has created ... site MusicMadeInMadison.com plays more than 450 songs written ... Burn, Infinite Helix and They Drive By Night. Founded in ... to help both the circulation of the artists and awareness ...
... a lot of Web developers realized some time ago: Flash ... be supported as one. , , In announcements made today ... current and future components of the Flash development platform like ... of course, is the Flash player itself, which is now ...
... and the Wisconsin Technology Council have released a report ... and the difficulties that still need to be addressed ... , ,Wisconsin Technology Council President Tom Still pointed ... Massachusetts have lost 10 to 15 percent of their ...
Cached Biology Technology:Flashing into the future 2Flashing into the future 3Report: Wisconsin still lags in college degrees held by workforce, leads elsewhere 2Report: Wisconsin still lags in college degrees held by workforce, leads elsewhere 3
(Date:7/11/2014)... When researchers at the National Institute of Standards ... do their work more effectively, they often prove ... Such was the case recently for M. Lorna ... in the growing specialization of glycomics. Glycomics is ... called glycans that are attached to proteins and ...
(Date:7/11/2014)... Ume University, Sweden, have explored two different ways ... sequence leading to the formation of oxygen molecules ... in the scientific journal Nature Communications . ... day synthetic catalysts for water oxidation, which are ... the direct storage of solar energy in fuels ...
(Date:7/11/2014)... that under the normal circumstances, astrocytes participate ... neuronal environment, and exhibit therapeutic and repairing ... Previous studies have found that nerve cells ... induction have reduced viability, which produces influences ... Yuan, Kailuan General Hospital, Hebei United University, ...
Breaking Biology News(10 mins):Molecular snapshots of oxygen formation in photosynthesis 2
... WI, MARCH 29, 2011 The environmental concern is ... the plant-soil relationship. Understanding plant,s absorption and accumulation of ... One highly carcinogenic contaminant commonly found in soil ... of the incomplete combustion of coal, oil, gas, and ...
... tell a story, these bleached bones that gleam in ... landscapes like Yellowstone may provide detailed accounts of how ... or even century, scientists have found. "The skeletons ... into our understanding of ecosystem history, especially how populations ...
... Researchers at the University of Michigan Comprehensive Cancer Center ... treat multiple types of cancer. A study, published this ... that AT-406 effectively targets proteins that block normal cell death ... while not harming normal cells. The researchers believe the drug ...
Cached Biology News:Bones of long-dead animals conjure ghosts at Yellowstone 2Bones of long-dead animals conjure ghosts at Yellowstone 3New cancer drug discovered at U-M heads to clinical trials 2
... has over 23 years of experience in ... have made antibodies to large and small ... antigens, and plasmid DNA-encoded antigens. We understand ... so our hybridoma development programs are never ...
... Rapid-Screen cDNA Library Panels were designed for ... sets of PCRs to identify the desired ... performed in a 96-well "Master Plate," where ... clones. Having identified the positive well(s) by ...
... The Rapid-Screen cDNA Library Panels were ... just three sets of PCRs to identify ... analysis is performed in a 96-well "Master ... from 5,000 clones. Having identified the positive ...
... CBED Maxi Kit provides a fast and ... protein solutions for buffer exchange, and for removing ... kit is based on spin-column chromatography using Norgens ... such as MgCl 2 , NaCl, KCl, CaCl ...
Biology Products: