Navigation Links
Salmonella typhimurium

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.532 01/2002 Microorganism Salmonella typhimurium LT2 Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pBR322 in TE buffer) Growth medium LB medium Washing solution 10 mM HEPES, pH 7.0; 10% glycerol Electroporation solution 10% glycerol Outgrowth medium SOC medium (without antibiotics) Cuvette 2 mm gap width Reference Binotto J., et al 1991 Canadian Journal of Microbiology 37 474-477 Making electrocompetent cells:

1. Incoculate a flask of LB 1:100 with a fresh overnight culture, grow at 37 C with shaking to an O.D.640 of 0.75. Chill the cells in an ice-water bath for 15 min. 2. Harvest by centrifugation (4,000 x g, 10 min., 4 C). 3. Wash one time in the original culture volume with HEPES and one time with 10% glycerol using 1/100 of the original volume. 4. Resuspend in 10% glycerol.
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Salmonella typhimurium
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Salmonella typhimurium

(Date:12/24/2014)... (PRWEB) December 23, 2014 Earlier this ... James L. Sherley, director of the Adult Stem Cell ... an often overlooked and under appreciated unique property of ... by Distributed Stem Cells: Misunderstood in the Past, Important ... to congress participants. He gave the address at ...
(Date:12/24/2014)... MD (PRWEB) December 23, 2014 ... into law H.R. 83, the Omnibus and Continuing ... first time, made hydrocephalus a condition eligible to ... Programs (CDMRP) administered by the Department of Defense ... with its allies on Capitol Hill, is celebrating ...
(Date:12/24/2014)... 23, 2014 The report ... as its definition, classification, application and industry ... manufacturing process, and product cost structure. Production ... The analysis also covers upstream raw materials, ... development trend and proposals. In the end, ...
(Date:12/24/2014)... The report expects global cell isolation and ... also provides a carefully analyzed data about the vital ... market. , Full Copy of Report @ http://bit.ly/1yUxy0T ... for cell expansion will keep witnessing growth at an ... to be driven by rapid technological advancements, government funding ...
Breaking Biology Technology:Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 3
... Wisconsin governor are playing a game of one- upsmanship on pocketbook ... to up the ante after Gov. Jim Doyle announced ... ethanol. , ,Green, however, did not take that opportunity to call ... once voted for legislation to repeal the controversial law. Instead, he ...
... of the Fox Broadcasting Company 's hit television program, ... preparing to exploit a consumer market catering to aspiring pop ... products for digital video, music, DVD, and audio production, has ... develop two software products based on the show, which won ...
... Wis. - Saying former employees began to develop Bone ... that purchased Bone Care in 2005 is suing three former ... , , Genzyme Corp . filed the suit in ... , claiming that the plaintiffs applied for patents on new ...
Cached Biology Technology:Green doesnt up the ante on markup law 2American Idol spurs music software 2Genzyme sues former Bone Care employees 2Genzyme sues former Bone Care employees 3
(Date:12/10/2014)... YORK , Dec. 8, 2014 You,ve been ... banking account but can,t remember your password, site key or ... Who was your first grade teacher? Today, ... the app that will finally put an end ... – 1U TM . 1U leverages a user,s ...
(Date:12/10/2014)... Research and Markets ( http://www.researchandmarkets.com/research/xclcvw/biometrics_market ... "Biometrics Market in Japan 2014-2018" report to ... The integration of biometrics and ... and upgradation of the driver,s license is one ... Besides the aforementioned projects, biometrics is being used ...
(Date:12/4/2014)... , Dec. 3, 2014  Crossmatch™, a ... announced that its DigitalPersona ® Pro Enterprise ... have been deployed throughout First Bank branch locations ... South Carolina and Virginia. First Bank, ... Southern Pines, North Carolina , selected ...
Breaking Biology News(10 mins):The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3
... and biological aspects of cellular self-organization are well-studied; less ... how their behavior and communication are affected by ... the University of California San Diego, in a paper ... of the National Academy of Sciences, have begun ...
... Japanese researchers were awarded the 2008 SNM Wagner-Torizuka ... States through 2010 as part of the two-year ... Medical College, undertaking a fellowship at Johns Hopkins ... the University of Washington, Seattle; Ryo Nakazato, Toho ...
... has clearly illustrated, the top priority for the next ... Believing that expanding the nation,s commitment to basic ... the next President can take to ensure our long-term ... questions (attached) to the moderator of tomorrow,s presidential debate. ...
Cached Biology News:UC San Diego bioengineers fill holes in science of cellular self-organization 22008 SNM Wagner-Torizuka Fellowships announced 22008 SNM Wagner-Torizuka Fellowships announced 3The Science Coalition's '10 questions' for the presidential debate 2
... PTOs are internucleotide modifications, where one ... replaced by a sulphur atom. This modification ... are available as Full-PTOs ... with thio-caps Several internucleotide ...
... and 135 S steam sterilizers with their ultra-flat ... advantages of a double-walled device - low energy ... drying by built-in jacket heater - with those ... chamber is free of furnishings and feed water ...
Request Info...
... T4 Polynucleotide Kinase (PNK) catalyzes the ... to the 5'-hydroxyl of single- and ... The enzyme also removes the 3'-phosphate ... deoxyribonucleoside 3', 5'-diphosphates to form a ...
Biology Products: