Navigation Links
Saccharomyces cerevisiae

entrifuge as above. Resuspend in 0.5 ml of 1 M sorbitol to a final volume of 1.0 to 1.5 ml, keep on ice.

Electroporation of cells:

  1. Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. Incubate 5 min on ice. Transfer mixture into a prechilled cuvette.
  2. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V) 1,500 V Time constant (T) 5 ms
  4. Immediately add 1 ml of cold sorbitol. Plate various aliquots onto selective plates containing 1 M sorbitol.
Expected results: Transformation efficiency up to 104 to 105 transformants/g of DNA. Note: It is possible to scale this protocol down by starting with a culture volume of 50 ml.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Saccharomyces cerevisiae
2. Saccharomyces cerevisiae
3. Saccharomyces cerevisiae
4. Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae
5. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Saccharomyces cerevisiae

(Date:7/10/2014)... July 10, 2014  Kainos Capital, a firm specializing ... announced that it has acquired the Slim-Fast brand from ... business. Terms of the transaction were not disclosed. ... replacement business that markets ready-to-drink shakes, powders, bars and ... and in the United Kingdom ...
(Date:7/10/2014)... 15, 2014 -- According to the International Atomic ... security comes from terrorists acquiring sufficient quantities of ... a crude nuclear explosive device. The IAEA also ... have involved gram-level quantities, which can be challenging ... to a new study appearing this week in ...
(Date:7/10/2014)... disorders has been extremely challenging because there are many ... to cause disease. Now investigators reporting in the July ... Stem Cell describe a strategy that may help ... risk factors or environmental exposures to affect the development ... variant that may predispose individuals to schizophrenia. , ...
(Date:7/10/2014)... out for crude 3-D glasses, polarized glasses, and ... basic devices, used to trick the brain into ... rendered obsolete with the introduction of new holography ... TAU doctoral students Yuval Yifat, Michal Eitan, and ... on nanoantennas that could be used for security ...
Breaking Biology Technology:Kainos Capital Acquires Slim-Fast From Unilever 2Sensitive detection method may help impede illicit nuclear trafficking 2New strategy could uncover genes at the root of psychiatric illnesses 2Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3
... April 24 Sinobiopharma, Inc. (OTC Bulletin Board: SNBP) ("Sinobiopharma" or, ... financial results for the nine month period ended February 28, 2009.Financial ... Sales revenue increased to US $2,715,702 for the ... corresponding period ended February 29, 2008. , ...
... 24 Dr. Susan Weinschenk is Chief of ... global leader of user experience design services. Dr. Weinschenk recently ... : What makes them click? ... decision makers immediately took note. Accolades about her book and ...
... -Testimony To Legislature Calls For Rejection Of Legislation That ... 24 In testimony to the Legislature and a ... largest biotechnology association warned that a bill under consideration ... and onerous regulatory environment for biotechnology growth and development ...
Cached Biology Technology:Sinobiopharma Reports a 141% Increase in Revenues From Unaudited Financial Results for the 9 Month Period Ended February 28, 2009 2Dr. Susan Weinschenk, Chief of Technical Staff and Services, Speaks on Neuro Web Design at 'Persuasive Technologies 2009 Conference' and 'Voices That Matter Web Design Conference' 2Regional Biotech Association Warns Legislation Would Make VT Least Friendly State in Nation for Biotechnology and Life Sciences 2
(Date:7/10/2014)... Society (ACS) announced today that Courtney Aldrich, Ph.D., ... Infectious Diseases as editor-in-chief. With the first ... pioneering journal will meet a growing demand for ... research. , "Our vision is to develop a ... on the basic science that advances the field ...
(Date:7/10/2014)... satellite spotted smoke over the U.S. Mid-West from ... , At 1200 UTC (8 a.m. EDT) on ... the brownish-colored haze created by forest fires in ... into South Dakota. This image was created by ... Flight Center, Greenbelt, Md. , According to ...
(Date:7/10/2014)... National Cancer Research Centre (CNIO) Macromolecular Crystallography Group ... protein called BuD to DNA in order to ... the researcher who led the study, says the ... the instructions contained in the genome to treat ... study is published in the journal Acta ...
Breaking Biology News(10 mins):ACS Infectious Diseases: Unique chemistry journal names editor 2CNIO scientists develop technology to redirect proteins towards specific areas of the genome 2
... AMES, Iowa Venture capitalist Vinod Khosla told last ... why he believes fuel blends containing 85 percent ethanol ... who last year told The New York Times ... companies developing ethanol production technologies ticked off several ...
... greatly increases the risk of heart attack and stroke ... the heart and blood vessels, researchers at the Saint Louis ... could lead to new therapies to treat or prevent heart ... States as well as answer key questions about other ...
... a prestigious American science journal proves that mercury atmospheric ... as three years. Biologists from the University of Alberta ... and carrying out the experiment. The study concludes ... other industrial activities were to be cut immediately, the ...
Cached Biology News:Iowa State University conference examines developing bioeconomy 2Research explains link between cholesterol and heart disease 2