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SUPERIOR RNA FOR DEMANDING APPLICATIONS

rat tissues and cell lines. The samples are processed using Ambion's RNA isolation reagents to recover highly pure, intact RNA. A stringent DNase treatment is included to destroy any contaminating genomic DNA, and to ensure that the RNA is ready for use in downstream applications such as RT-PCR. Although it is impossible to remove every trace of DNA contamination from an RNA sample, FirstChoice RNAs are tested by real-time PCR to ensure that residual DNA contamination is insignificant (<0.05%). Additionally, the RNAs undergo rigorous testing for nucleases. The integrity and stability of each RNA is verified by capillary electrophoresis using the Agilent 2100 bioanalyzer as shown in Figure 2.


The Purist Poly(A) RNA Available
FirstChoice Poly(A) RNA is isolated from the FirstChoice Total RNA described above. The RNA undergoes poly(A) selection using Ambion's Poly(A)Purist technology; a method that outperforms other purification methods and yields RNA that is more highly enriched for poly(A) RNA and is nearly devoid of rRNA. Because of the high quality of starting material (FirstChoice Total RNA), and the unsurpassed level of mRNA enrichment, 5 g of Ambion's FirstChoice Poly(A) RNA contains more mRNA than other commercially available poly(A) RNA preparations which are often highly contaminated with rRNA, DNA or both. An electropherogram of FirstChoice Poly(A) RNA is shown in Figure 4; it illustrates the purity of the preparation.


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