Navigation Links
SUPERIOR RNA FOR DEMANDING APPLICATIONS

rat tissues and cell lines. The samples are processed using Ambion's RNA isolation reagents to recover highly pure, intact RNA. A stringent DNase treatment is included to destroy any contaminating genomic DNA, and to ensure that the RNA is ready for use in downstream applications such as RT-PCR. Although it is impossible to remove every trace of DNA contamination from an RNA sample, FirstChoice RNAs are tested by real-time PCR to ensure that residual DNA contamination is insignificant (<0.05%). Additionally, the RNAs undergo rigorous testing for nucleases. The integrity and stability of each RNA is verified by capillary electrophoresis using the Agilent 2100 bioanalyzer as shown in Figure 2.


The Purist Poly(A) RNA Available
FirstChoice Poly(A) RNA is isolated from the FirstChoice Total RNA described above. The RNA undergoes poly(A) selection using Ambion's Poly(A)Purist technology; a method that outperforms other purification methods and yields RNA that is more highly enriched for poly(A) RNA and is nearly devoid of rRNA. Because of the high quality of starting material (FirstChoice Total RNA), and the unsurpassed level of mRNA enrichment, 5 g of Ambion's FirstChoice Poly(A) RNA contains more mRNA than other commercially available poly(A) RNA preparations which are often highly contaminated with rRNA, DNA or both. An electropherogram of FirstChoice Poly(A) RNA is shown in Figure 4; it illustrates the purity of the preparation.


'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Rapidly Synthesize Large Amounts of Capped RNA
2. Yeast Protein Production System Features High Yields and One-Step Purification
3. ArrayScript RT: Maximize Yields of Full-length cDNA
4. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
5. Translate High Yields of Active Protein
6. Translate High Yields of Biologically Active Proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: SUPERIOR RNA FOR DEMANDING APPLICATIONS

(Date:8/20/2014)... 20, 2014 /PRNewswire-iReach/ -- A case study from ... the school,s bioengineering department and the Intel® Internet ... Software Academic Program, UCSD,s research focuses on exploring ... body.  Photo - http://photos.prnewswire.com/prnh/20140819/137540 ... work of Dr. Todd P. Coleman , ...
(Date:8/19/2014)... One of the major limitations ... inadequate vascular supply. Nutrient and gas transport throughout ... critical for successful regeneration and integration into the ... in vascularization and promote angiogenesis in a variety ... design and architecture, and enhancing scaffolds with stem ...
(Date:8/19/2014)... IRVINE, Calif. (PRWEB) August 19, 2014 ... Indiegogo campaign to raise $200,000 to fund a ... diagnostic device. , This development is a ... one-size-fits-all model of care to the implementation of ... patient-centric method of practicing medicine. , Indiegogo contributions ...
(Date:8/19/2014)... 19, 2014 Research and ... the "Spectroscopy Equipment and Accessories - ... their offering. This report ... and Accessories in US$ Thousands by the ... Raman Spectrometers, NMR-EPR Spectrometers, Near Infrared (NIR) ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Vascularization in Tissue Engineering Highlighted at 12th New Jersey Symposium on Biomaterials Science 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 3Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2
... InterWest Partners,announced today the appointment of ... firm,s Advisory Committee: Caltech,president Dr. Jean-Lou Chameau, ... commissioner Dr. Mark McClellan. The three will ... strategic guidance,to the firm and its portfolio ...
... S. Young awarded "Most Innovative" award by the Association of ... ... Research Inc. (TSX:,ARI), a biotechnology company discovering and developing the ... Young, President,and Chief Executive Officer, received the "Most Innovative" award ...
... to dramatically reduce their dose of these often harsh ... Medicine in St. Louis have taken a step closer ... drug directly on tumors in rabbits using drug-coated nanoparticles. ... than used previously for this purpose markedly slowed tumor ...
Cached Biology Technology:Prestigious Scientific Leaders Join InterWest Advisory Committee 2Prestigious Scientific Leaders Join InterWest Advisory Committee 3Prestigious Scientific Leaders Join InterWest Advisory Committee 4ARIUS recognized for entrepreneurial excellence 2Nanosized technology has supersized effect on tumors 2Nanosized technology has supersized effect on tumors 3
(Date:8/20/2014)... During the past few years, Virginia Tech,s Wu Feng ... Microsoft grant from the "Computing in the Cloud" program, ... a $6 million award from the Air Force on ... from NSF and the National Institutes of Health on ... wove together the "parallel computing" aspects from each grant, ...
(Date:8/20/2014)... of honeybees grows to about 4,000 members, it triggers ... building of a special type of comb used for ... from the Department of Neurobiology and Behaviour at Cornell ... reproductive cycle of honeybee colonies. The results are published ... Nature . , Reproduction isn,t always a honeybee ...
(Date:8/20/2014)... 2014  The second annual Biometrics UnPlugged Executive ... Tampa, Florida on September 15, 2014, ... Technology and Strategy at Samsung, and Philippe de ... of this year,s event is Mobility at the ... "Biometrics UnPlugged: Mobility at the Crossroads of Commerce ...
Breaking Biology News(10 mins):The internet was delivered to the masses; parallel computing is not far behind 2The internet was delivered to the masses; parallel computing is not far behind 3The internet was delivered to the masses; parallel computing is not far behind 4Worker bees 'know' when to invest in their reproductive future 2Biometrics UnPlugged: Mobility at the Crossroads of Commerce & Privacy to Feature Interactive Sessions with Samsung and Experian 2
... an inherited structural mechanism that can make drugs ... mechanism decreases a protein and in turn causes ... therapies are used to treat patients with childhood ... researchers say their finding advances the field of ...
... may account for many cases of immune deficiency, in ... and Common Variable Immunodeficiency (CVID), report researchers in the ... to a new diagnostic test for these conditions, which ... unrecognized because of a lack of good tests. , ...
... Broken sewers, flooded industrial plants and dead bodies are all likely ... , But the water - and the muck it is leaving ... as it is unexpected: Toxins common in most urban environments that ... atop the city. , So says a University of Florida professor ...
Cached Biology News:Genomics researchers discover protein deficit that causes drug toxicity 2Protein amplification in melanoma is possible drug target 2Toxic flood lifts lid on common urban pollution problem 2Toxic flood lifts lid on common urban pollution problem 3
... VersArray ChipWriter Pro system, 220-240 V, uses ... proteins, oligonucleotides, carbohydrates, lipids, and other biomolecules ... applications. This system can generate high-density arrays ... slide or greater than 57,000 spots per ...
... The VersArray ChipWriter Pro system with ... printing technology to array DNA, proteins, oligonucleotides, ... variety of substrates for microarray applications. This ... colonies as small as 300 micrometers in ...
Thermal printer paper is used with the thermal printer for the VersaFluor fluorometry systems. Supplied as 10 rolls....
The Freeze 'N Squeeze DNA gel extraction kit uses a quick freeze-and-thaw cycle and centrifugation to draw 50-23,000 bp DNA out of agarose gel slices. The kit includes materials for 100 preps....
Biology Products: