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SUPERIOR RNA FOR DEMANDING APPLICATIONS

community to ethically obtain tissues in a manner that preserves the integrity of the RNA. We have also optimized RNA isolation procedures specifically for brain and neural tissue. These efforts, in conjunction with stringent quality control standards, ensure that FirstChoice Brain Total RNA is intact, stable, and free of genomic DNA (<0.05%). Results from one of our Quality Control assays are shown in Figure 2.

Figure 2. FirstChoice Human Brain Total RNA Stability. RNA was stored overnight at either -20C or 37C. The RNA was then evaluated on an Agilent 2100 bioanalyzer. The approximate 2:1 ratio of the peaks representing 28S and 18S rRNA indicates that the RNA is intact and stable even when stored overnight at 37C.


Replicate samples of total brain RNA were stored overnight at either 20C or at 37C and then analyzed by capillary electrophoresis using the Agilent 2100 bioanalyzer. Even after overnight incubation at 37C, the ratio of the areas of the peaks representing 28S and 18S rRNA are approximately 2:1; this illustrates the high quality and stability of FirstChoice RNA. Total RNA is currently available from the following regions of the brain: frontal cortex, occipital cortex, pariental cortex, temporal cortex, basal ganglia, cerebellum, medulla, and thalamus.


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