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SUPERIOR RNA FOR DEMANDING APPLICATIONS


With the emergence of more and more sophisticated techniques for gene expression analysis (e.g. array analysis, real-time RT-PCR), the quality of the RNA used in these studies has become increasingly critical. As The RNA Company, Ambion is committed to defining what "high quality RNA" truly means and providing the best RNA available from any source. Ambion's newest RNA products include RNA from specific regions of the human brain and other human RNAs from diverse normal and diseased tissues.


RNA Isolation: Leave it to Ambion
Obtaining high quality RNA, especially human RNA, can be challenging because of factors that are often difficult to control. Tissue procurement is probably the biggest hurdle for many sample types. First a reputable and ethical tissue source must be identified. Typically you then wait for the desired tissue type and sample size to become available, and finally you hope that the tissue was harvested quickly and handled properly to avoid RNA degradation. Once you have obtained the desired tissue sample, it must be disrupted and homogenized using the technique and reagents best suited to the tissue source. After completing the RNA isolation procedure, it is also crucial for demanding applications to assess the quantity, quality, and purity of the RNA. For successful gene expression analysis and cloning experiments, accurately quantitated, full length, pure RNA is required. Many applications also demand that the RNA be purified of contaminants such as genomic DNA, ribosomal RNA (in mRNA preps), p
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