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SPRINTPREP SINGLE STEP PLASMID PURIFICATION

variety of plasmids including pOT, pDNR, pFLCIII, pZErO and pBluescript. Figure 2 demonstrates the uniform purification of a variety of plasmids.



REPRODUCIBLE AND ROBUST 384-WELL PURIFICATION

SprintPrep produces uniform plasmid purification without the need for normalization of cell number (Figure 3). 384 individual plasmids were purified from 150 l of cultures grown for 16 hours. Despite well to well variations in initial cell concentration, all 384 purifications produced consistent plasmid yields.



HIGH QUALITY SEQUENCING RESULTS
SprintPreps direct purification protocol produces superior sequencing results. Plasmid DNA purified using SprintPrep routinely produces Phred20 read lengths greater than 650bp. Figure 4 summarizes results for a Chimpanzee sequencing project conducted at Agencourt. The average Phred20 read length for over 11,000 reads was 624bp. The high quality and uniform quantity of purified DNA leads to higher and more even sequencing signals.



SPRINTPREP AND THE MAMMALIAN GENE COLLECTION (MGC)
SprintPrep has been validated on more than 15,000 cDNA clones as part of the Mammalian Gene Collection. Purified plasmids were sequenced using 30ng of template DNA in a 1/16th BigDye v3.1 terminator reaction. Unincorporated dye-terminators were removed using CleanSEQ and sequences read on ABI 3700 and 3730xl's. Overall project results are summarized in the table below and a typical 3700 electropherogram is shown in Figure 5. Agencourt has submitted over 600,000 cDNA sequences to GenBank as part of the MGC and continues to do large-scale cD
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Related biology technology :

1. SINGLE PASS SEQUENCING
2. COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION
3. AMPURE PURIFICATION SYSTEM
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