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SPRINTPREP SINGLE STEP PLASMID PURIFICATION

SprintPrep is an efficient SPRI-based plasmid purification system that can be completed in less than 15 minutes. Unlike most traditional plasmid purification chemistries, SprintPrep isolates plasmid DNA directly from overnight cultures, which can improve the turnaround time of a sequencing pipeline by as much as a full day. SprintPreps simple protocol requires no sample transfers, no centrifugation or filtration and is easily automated in both 96 or 384-well formats.

SPRINTPREP FEATURES

EASE OF USE
  • No cell pelleting, resuspension or
  • lysate clearing
  • Perform the prep in the culture plate

    SPEED
  • 15 minute prep
  • Streamlines sequencing pipeline


  • HIGH QUALITY SEQUENCING RESULTS
  • >650 Phred20 read lenghts
  • >90% pass rates

    FLEXIBILITY
  • 96 or 384-well formats
  • Manual or fully automated protocol



    UNSURPASSED EASE
    SprintPrep generates sequence data in under 30 hours with only a single plate transfer (Figure 1). Traditional filtration-based plasmid purification and rolling circle amplification reactions require multiple plate transfers or labor-intensive cell pelleting and lysate clearing steps.



    SIMPLE PLASMID PURIFICATION
    Unlike other purification technologies that require sample transfer and labor intensive centrifugation or filtration steps, SprintPrep isolates plasmid DNA directly from overnight cultures. SprintPrep was validated using a
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