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SAGE & LONGSAGE SEQUENCING

Serial Analysis of Gene Expression (SAGE) is a powerful genome-wide expression profiling technology. Agencourt offers a cost-effective outsourcing solution for sequencing of both SAGE and LongSAGE libraries to make SAGE technology more accessible to researchers. Agencourt's SAGE sequencing service provides our clients with:

  • Rapid turnaround

  • Long Phred20 read lengths

  • Stringent control of sample and data integrity

  • Proven SAGE sequencing experience


  • SAGE SEQUENCING SERVICE

    Our SAGE sequencing service includes:

  • Template purification
  • Sequence reaction set up and thermocycling
  • Dye-terminator removal
  • Sequence delineation on an ABI PRISM 3700/3730xl
  • Data delivery via a secure FTP site
  • HIGH QUALITY SEQUENCING RESULTS

    Our fully-automated genomic pipeline and SPRI nucleic acid purification technology allow us to process over 20 million Phred20 bases per day. We use this large throughput capacity to rapidly perform SAGE sequencing with industry-leading quality. Our long Phred20 read lengths and high sequencing pass rates allow us to obtain high quality sequence data for approximately 40 SAGE tags or 35 LongSAGE tags per read.

    PROVEN SAGE EXPERIENCE
    Agencourts proven experience in sequencing SAGE libraries sets our service center apart. We perform

    sequencing for some of the world's preeminent SAGE research groups and projects, including:

  • Johns Hop kins Kemmel Cancer Center developers of SAGE technology
  • Genzyme holds exclusive rights to SAGE technology
  • NCI's Cancer Genome Anatomy Project

    CANCER GENOME ANATOMY PROJECT

  • The goal of CGAP is to analyze gene expression profiles of normal, precancer, and cancer cells in an effort to improve the detection, diagnosis, and treatment of cancer. Agencourt is the exclusive provider of SAGE sequencing for CGAP. To date, we have sequenced SAGE libraries derived from over 20 distinct tumor types.

    ADDITIONAL SAGE RESOURCES
    For additional information on SAGE and LongSAGE technologies, please refer to the following web sites: www.genzyme.com/sage, www.invitrogen.com and www.sagenet.org
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    4. SINGLE PASS SEQUENCING
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