( ...)S.E.T.S. Swab Extraction Tube System

A New Tool for the Rapid and Efficient Extraction from Swabs in Research Samples

Swabs are commonly used for sample extraction from a variety of sites, and for the subsequent detection of infectious agents (bacteria, fungi, or viruses). After the swab procedure, the infectious agents are typically released into a culture medium or onto a culture plate.

Modern molecular biology methods, such as the detection of bacteria or viruses by polymerase chain reaction (PCR) require a different approach in the downstream handling of the swab. The goal is to recover as much of the material adhering to the swab as possible without further diluting it and without introducing potentially inhibitory substances.

Product Description

Roche Applied Science has developed a highly convenient plastic disposable that can be used to recover adhering material from a swab using a very straightforward procedure: the S.E.T.S., an acronym for Swab Extraction Tube System (Figure 1).



The S.E.T.S. is designed to hold standard swab tips (approximately 5 8 mm in diameter and 1025 mm in total length) in the Inner tube (1). The Inner tube has a hole with a defined diameter on the bottom. The lid is then closed, and the loaded Inner tube is placed into the Collection tube (2). The assembled device is now placed in a standard benchtop centrifuge for a brief spinning down of the adherent material from the swab through the hole in the Inner tube. After spinning down the material, the closed Inner tube with the swab is discarded.

Benefits

Flexibility

• Virtually any sample material collected by swab is fully compatible with subsequent nucleic acid extraction procedures (e.g., boiling prep or extraction on the MagNA Pure LC Instrument).
  
  

Speed

• From swab to collected material, ready for nucleic acid purification in less than five minutes.
  
  

Safety

• Swab with potentially infectious material is handled in a closed tube.
  
  

The Collection tube with the collected material is now closed with the screw cap (3), and is ready for storage or further nucleic acid extraction. The S.E.T.S. will support direct boiling preparation of the collected material in the closed outer tube and is compatible with the MagNA Pure LC Instrument.



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