( . Blood and milk are ideal s...)Robust High Throughput RNA Isolation from Blood Samples

. Blood and milk are ideal sample sources for BVDV RNA isolation due to the simplicity and ease of sample collection. BVDV positive and negative bovine blood samples were aliquoted (50 l) into separate wells of a 96 well plate and processed using the MagMAX-96 Blood protocol on a Biomek 2000 (Beckman Coulter). Control RNA was spiked into each sample well at equal copy numbers to monitor sample processing consistency of the 96 well plate. The purified RNA was subsequently used for qRT-PCR targeting BVDV RNA and control RNA. BVDV target amplification was highly consistent: average Ct=21.4 0.5. Control RNA amplification was equivalent (average Ct=28.3 0.4) across the 96 samples. No cross contamination was observed. Processing a full plate of samples requires ~1 hr.

The MagMAX-96 Blood protocol permits rapid (<1 hr) total and viral RNA isolation from blood samples (up to 50 l) and is also applicable for viral RNA isolation from milk samples. The RNA is eluted in a low salt buffer (2050 l) and can be directly used for qRT-PCR.

Scientific Contributors

Angela Burrell, Quoc Hoang, Roy Chris Willis, WeiWei Xu, Mangkey Bounpheng, Xingwang Fang Ambion, Inc.

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Ordering Information
Cat# Product Name Size 1837 MagMAX-96 Blood RNA Isolation Kit 96 purifications
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