Navigation Links
Robust High Throughput RNA Isolation from Blood Samples

ssays. Total RNA (~25 ng) was isolated with the MagMAX-96 Blood Kit and was used in qRT-PCR targeting the TATA Binding Protein (TBP, panel A) and RNA Polymerase II (RPII, panel B) mRNA [(+)RT reactions] and genes [()RT reactions]. Minimal genomic DNA contamination was revealed by the Ct values of (+)RT and ()RT reactions. Similar total RNA yield and quality were obtained from fresh and blood stored at 4C (for 24 hr) as demonstrated by similar (+)RT Ct values.


RNA isolated from blood stored at -80C had similar yields but lower integrity than samples from fresh blood or blood stored at 4C (data not shown). RNA degradation may result from hemolysis during thawing; however, the RNA can still be used for successful qRT-PCR yielding valuable gene expression profiling.


High Throughput Isolation of Blood: RNA for Veterinary Molecular Diagnosis

The ability to directly use whole blood for total and viral RNA isolation decreases cross contamination and saves time and resources. This new kit is designed for high throughput viral and total RNA isolation of 50 l samples in a 96 well plate. The MagMAX-96 Blood RNA Isolation Kit has been used successfully for total and viral RNA from bovine (Figure 3), porcine, and ovine whole blood, as well as bovine milk samples, allowing high throughput viral, veterinary molecular diagnosis.

Figure 3. MagMAX-96 Blood Is Easily Adaptable for Automation. Bovine viral diarrhea virus (BVDV) infection is a major problem of worldwide distribution that results in economic losses for the beef and dairy industries
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
3. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
4. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
5. Highest Possible Transformation Efficiencies for High-Throughput Applications
6. High-Throughput Cultivation of Bacterial Clones
7. High-Throughput Isolation of Total RNA
8. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
9. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
10. High Throughput aRNA Amplification
11. High Throughput siRNA Electroporation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Robust High Throughput RNA Isolation from Blood Samples

(Date:7/30/2014)... AZ (PRWEB) July 30, 2014 Sales ... the launch of blended sales training programs for companies ... sales training programs used by thousands of salespeople over ... blended sales training program can be customized to address ... , The blended sales training program consists ...
(Date:7/30/2014)... STONY BROOK, NY (PRWEB) July 30, 2014 ... (OTCQB: APDN), (Twitter: @APDN), a biotechnology firm that provides ... Dr. Michael E. Hogan, Ph.D. Vice President of Life ... in mid-September. , Dr. Hogan will lead ... to form business relationships with biotech and healthcare customers, ...
(Date:7/30/2014)... Yuma Regional Cancer Center Officially ... brings comprehensive cancer care to Yuma, Arizona. , ... Center has officially opened. A Mayo Clinic Care ... such as open, semi-private and private infusion rooms, ... Resource Center, hematology, rehabilitation center, a pain management ...
(Date:7/30/2014)... COLLEGE PARK, Md. , July 30, 2014 ... with biological components to interrogate biology, but such ... control biology. As such, researchers ... cells for in vitro metabolic engineering ... metabolism on a chip. New devices are envisioned ...
Breaking Biology Technology:Sales Horizons Launches New Blended Sales Training Programs to Companies Engaged in Complex B2B Sales 2Sales Horizons Launches New Blended Sales Training Programs to Companies Engaged in Complex B2B Sales 3Dr. Michael E. Hogan, Ph.D., Joins Applied DNA Sciences as Vice President, Life Sciences 2Dr. Michael E. Hogan, Ph.D., Joins Applied DNA Sciences as Vice President, Life Sciences 3Sate-of-the-Art Yuma Regional Cancer Center Officially Opens 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4
... 4 Lentigen Corporation, a biotechnology company specializing ... delivery technologies, and Expression Therapeutics, a biotechnology company ... collaborative agreement to co-develop novel cell and protein ... blood clotting disorder caused by a mutation in ...
... ) announced today that it will release its fourth quarter ... 24, 2009. The Company will host a telephone conference call ... Eastern Time. A question and answer session will follow. Conference ... conference call, interested parties in the United States and Canada ...
... ST. LOUIS, Feb. 4 On the heels of a ... MON ) executives will explain to investors this month where ... rest of this year and how it plans to achieve ... profit level by fiscal 2012. Terry Crews, chief financial officer, ...
Cached Biology Technology:Lentigen and Expression Therapeutics Sign Hemophilia Co-Development Agreement 2Lentigen and Expression Therapeutics Sign Hemophilia Co-Development Agreement 3Kendle Announces Details Regarding Fourth Quarter and Full-Year 2008 Earnings and 2009 Guidance Release, Webcast and Conference Call 2Monsanto Executives to Discuss Company's Growth Drivers, Profit Goals at Investor Conferences in February 2
(Date:7/30/2014)... in cancer treatment has been shown to relieve ... for neural transmission between the peripheral and central ... Institute of Radiation Medicine, Chinese Academy of Medical ... into rat dorsal root ganglia (DRG) to provide ... radioactivity (0, 14.8, 29.6 MBq) were implanted separately ...
(Date:7/30/2014)... JOSE, Calif. , July 30, 2014 /PRNewswire/ ... partnership with the United States Air Force Research ... proposed by Binghamton University ... human performance monitoring (HPM). The $425,000 project, with ... electronics packaging firm i3 Electronics, Inc. ( ...
(Date:7/29/2014)... USA The advanced optical methods and applications for ... a revolution in the neurosciences were the inspiration behind ... SPIE, the international society for optics and photonics. , ... BRAIN Initiative in the United States, and a special ... and provide examples of the impact that optics and ...
Breaking Biology News(10 mins):Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 2Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 3SPIE launches Neurophotonics journal with special section on BRAIN Initiative 2SPIE launches Neurophotonics journal with special section on BRAIN Initiative 3
... and Avalon Technology partner on new ... aviation-related ... Inc.,(OTC Bulletin Board: CTHH) ( http://www.catcherinc.com ), a leader ... Technology ( http://www.avalontechnology.com ), a leading,integrator of managed mobile ...
... resulting from higher CO2 could boost the amount ... new study looks at a poorly understood process ... Sayer, postdoctoral fellow at the Smithsonian Tropical Research ... University of Minnesotas Department of Ecology, Evolution and ...
... Boulder, CO, USA Two hundred years ago Lewis and ... deal of scientific research with its descriptions of territory acquired ... be disappointed by the absence of living megafauna, the area ... volume published by the Geological Society of America makes clear ...
Cached Biology News:CATCHER to be Deployed at Major U.S. Airline 2CATCHER to be Deployed at Major U.S. Airline 3CATCHER to be Deployed at Major U.S. Airline 4Complex carbon picture clearer 2
... The LTQ XL extends the legendary ... techniques to generate structural information through Thermo ... Thermo Scientific's LTQ XL is the only ... PQD, ETD and CID. Pulsed Q ...
... Varian, Inc.'s CP-4900 is a ... always delivers the information you ... When the composition of gas ... generation micro-Gas Chromatograph generates more ...
... The PolarScreen Peroxisome Proliferator-Activated Receptor-gamma ... a sensitive and efficient method ... PPARGamma ligands using fluorescence polarization ... PPARGamma-Ligand binding domain (PPARGamma-LBD) with ...
The essence of fluorescence analysis is sensitivity. The high throughput optical system in the RF-5301PC employs a blazed holographic grating, photomultiplier and digital signal processing to provide...
Biology Products: