( ssays. Total RNA (~25 ng) was isolated...)Robust High Throughput RNA Isolation from Blood Samples

ssays. Total RNA (~25 ng) was isolated with the MagMAX-96 Blood Kit and was used in qRT-PCR targeting the TATA Binding Protein (TBP, panel A) and RNA Polymerase II (RPII, panel B) mRNA [(+)RT reactions] and genes [()RT reactions]. Minimal genomic DNA contamination was revealed by the Ct values of (+)RT and ()RT reactions. Similar total RNA yield and quality were obtained from fresh and blood stored at 4C (for 24 hr) as demonstrated by similar (+)RT Ct values.

RNA isolated from blood stored at -80C had similar yields but lower integrity than samples from fresh blood or blood stored at 4C (data not shown). RNA degradation may result from hemolysis during thawing; however, the RNA can still be used for successful qRT-PCR yielding valuable gene expression profiling.

High Throughput Isolation of Blood: RNA for Veterinary Molecular Diagnosis

The ability to directly use whole blood for total and viral RNA isolation decreases cross contamination and saves time and resources. This new kit is designed for high throughput viral and total RNA isolation of 50 l samples in a 96 well plate. The MagMAX-96 Blood RNA Isolation Kit has been used successfully for total and viral RNA from bovine (Figure 3), porcine, and ovine whole blood, as well as bovine milk samples, allowing high throughput viral, veterinary molecular diagnosis.

Figure 3. MagMAX-96 Blood Is Easily Adaptable for Automation. Bovine viral diarrhea virus (BVDV) infection is a major problem of worldwide distribution that results in economic losses for the beef and dairy industries
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