Navigation Links
Robust High Throughput RNA Isolation from Blood Samples

which involves tissue/cell lysis and magnetic bead sample capture followed by TURBO DNase treatment. High quality, intact RNA with 28S/18S rRNA ratios >0.9 (Agilent 2100 bioanalyzer) was obtained (Figure 1). RNA yield was determined by A260 with an average range of 0.310.52 g total RNA from all samples (50 l each).

Figure 1. MagMAX-96 Blood Consistently Recovers Intact Total RNA from Fresh Blood and Blood Stored at 4C. Human whole blood from four different donors was collected into EDTAVacutainer tubes; aliquots were stored at 4C for 24 hr. Fresh whole blood was collected from the same donors the following day for concurrent RNA isolation with the samples stored at 4C. Total RNA was isolated from 50 l blood from multiple donors in 8 replicates using the MagMAX-96 Blood Kit, and total RNA (~50 ng) from representative samples was analyzed on an Agilent 2100 bioanalyzer (A). RNA yield was determined by A260 measurement using the NanoDrop ND-1000A Spectrophotometer (B).


Genomic DNA contamination was determined by one-step qRT-PCR targeting hTBP (human TATA binding protein) and RPII (RNA polymerase II) mRNAs [(+)RT reactions] and genes [(-)RT reactions]. Total RNA purified from all samples contained minimal genomic DNA contamination as assessed by delta-Ct values from qRT-PCR (Figure 2). Similar (+)RT Ct values of qRT-PCR (hTBP and RPII) using RNA isolated from fresh blood and blood stored at 4C were obtained.

Figure 2. Comparison of Total RNA Isolated from Fresh Blood and Blood Stored at 4C in qRT-PCR A
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
2. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
3. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
4. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
5. Highest Possible Transformation Efficiencies for High-Throughput Applications
6. High-Throughput Cultivation of Bacterial Clones
7. High-Throughput Isolation of Total RNA
8. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
9. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
10. High Throughput aRNA Amplification
11. High Throughput siRNA Electroporation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Robust High Throughput RNA Isolation from Blood Samples

(Date:7/24/2014)... San Francisco, CA (PRWEB) July 24, 2014 ... Wednesday to be semi-finalists in Livestrong’s Big C Competition. ... Decisive Health are each headed to the semi-final round. ... in Livestrong’s exclusive three-month accelerator program, complete with mentoring ... country. , As part of the angelMD commitment ...
(Date:7/24/2014)... 2014  Neogen Corporation (Nasdaq: NEOG ) ... potential revenue from new rodenticide research are premature. In ... on July 22, 2014, Neogen,s CEO commented about several ... "It was my intent Tuesday at our ... a new type of rodenticide, but certainly not to ...
(Date:7/24/2014)... 24, 2014 1000 balances – 4 ... on customers’ weighing needs and expertly narrows down the ... balances, scales or even terminals. , First, select ... weight (or select readability instead) and define the accuracy ... selection criteria narrows down the list to models that ...
(Date:7/24/2014)... (PRWEB) July 24, 2014 Draper ... , is proud to announce that for 6 ... different countries will converge on the Draper University ... an intensive, experiential program focused on entrepreneurship, building ... selective school, currently running its sixth cohort, continues ...
Breaking Biology Technology:3 angelMD Startups Make Livestrong Big C Semi-finals 2Neogen comments on SenesTech 2Summer Entrepreneurship Program Kicked Off by Draper University in Silicon Valley 2Summer Entrepreneurship Program Kicked Off by Draper University in Silicon Valley 3
... 15 The Vical Incorporated,(Nasdaq: VICL ) ... on,Monday, September 22, at 1 p.m. Eastern Daylight ... through the Events page at http://www.vical.com ., ... biopharmaceutical products based on its,patented DNA delivery technologies ...
... BioMed Realty Trust, Inc.,(NYSE: BMR ) today announced ... dividend of $0.335 per share of common stock. The,dividend is ... BioMed also announced that its board of directors has ... Series A Cumulative,Redeemable Preferred Stock for the period from July ...
... of Institutional Investors to Attend Conference, MONTREAL, ... ("ProMetic"), today announced that Mr. Pierre Laurin, President,and ... Ford,s 5th,annual Investor Summit on September 16, 2008 ... the Mark Hopkins InterContinental Hotel in San Francisco., ...
Cached Biology Technology:Vical to Present at UBS Global Life Sciences Conference 2BioMed Realty Trust Declares Third Quarter 2008 Common Stock and Preferred Stock Dividends 2ProMetic to present at Merriman Curhan Ford's Investor Summit 2008 on September 16th 2
(Date:7/25/2014)... engineering at the University of Houston, has written ... organ development., "Introduction to Tissue Engineering: Applications and ... the field of artificial organ development. Metin Akay, ... UH, served as a series editor on the ... published books on the subject of growing artificial ...
(Date:7/25/2014)... (NRG1) is a pleiotropic factor characterized by the ... of exons that confer to the protein deeply ... both the myelination occurring during development and the ... nerve: axon degeneration, axon regrowth, remyelination and target ... have discovered that the soluble NRG1 upregulation observed ...
(Date:7/25/2014)... that the age girls reach puberty is influenced by ,imprinted ... parent contributes the gene. This is the first evidence ... birth and details of this study were published today in ... period, known as menarche, is a marker for the timing ... onset of menses varies between girls, is an inherited trait, ...
Breaking Biology News(10 mins):University of Houston researcher publishes textbook on tissue engineering 2Could age of first period influence development of diseases in older women? 2
... Oct. 16, 2012  Today 23andMe, the leading personal ... as Vice President Communications, Neil Rothstein as Vice ... Strategic Alliances joining the company,s executive leadership team. ... Castro is responsible for the company,s corporate communications, ...
... MedNet Solutions, a global life sciences technology solutions ... & Data Corporation (SDC), a specialized Contract Research Organization ... services to the life sciences industry, are pleased to ... ™ EDC platform as their eClinical ...
... MOUNTAIN VIEW, Calif. and RAANANA, Israel, Oct. 23, ... ), the leading licensor of silicon intellectual property ... Ltd, a global supplier of embedded speech processing ... deliver enhanced speech processing solutions for smart devices, ...
Cached Biology News:23andMe Names Christine Castro, Neil Rothstein and Jonathan Ward to Leadership Positions 2Statistics & Data Corporation Selects MedNet Solutions As Strategic Technology Partner 2Statistics & Data Corporation Selects MedNet Solutions As Strategic Technology Partner 3CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs 2CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs 3
... Proteolytic degradation is critical to the ... and regulatory proteins as important and ... metabolism, heat shock and stress response, ... receptors and ion channels, cell cycle ...
... scanner serves as the spot-picking platform. This ... rack positions on the tray, leaving space ... on the remaining two racks.,The software allows ... mouse-click on the scanned gel image. The ...
...
... configured specifically for pharmaceutical powder packaging, ... are precision-joined to create ultra-smooth surfaces ... and disinfect. One-inch radius corners simplify ... strong, rigid surface without external bracing. ...
Biology Products: