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Robust High Throughput RNA Isolation from Blood Samples

MagMAX-96 Blood RNA Isolation Kit
Mammalian whole blood is a widely used tissue for molecular assays due to abundant genetic information as well as ease and simplicity of sample collection. RNA isolation from whole blood is challenging, but Ambions MagMAX-96 Blood RNA Isolation Kit uses strong denaturing lysis conditions and proteases to effectively inactivate ribonucleases and remove blood proteins.


MagMAX-96 Blood Technology

For RNA purification, Ambions magnetic bead-based MagMAX technology provides a fast, simple protocol that eliminates the use of organic solvents as well as problems associated with glass fiber filter-based methods (e.g., filter clogging and inconsistent RNA yield). Whole blood (and milk) samples can be processed manually using multichannel pipettes or adapted for automation to recover total and viral RNA of high yield, quality, and purity. The extracted RNA is ideal for downstream molecular diagnostics and gene expression profiling using quantitative reverse transcription-polymerase chain reaction (qRT-PCR).


Recover High Quality Total RNA from Blood

High quality total RNA from human whole blood (fresh or stored at 4C) can be easily and efficiently recovered using MagMAX-96 Blood. For example, total RNA (8 replicates from multiple donors) was isolated using the standard MagMAX-96 Blood protocol,
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