Navigation Links
Reverse transcriptases suitable for two-step RT-PCRd

Product size Type of primers Primer anneal. temp. Sensitivitye Specificityf Fidelity in RT GC-rich structures Product to selectg total RNA viral RNA specific primer oligo dt primer up to 1kb specific 55-70C n.d. n.d. n.d. n.d. - n.d. Tth DNA Polymerase up to 4kb specific, oligo(dT) hexamers 55-70C + + (+) ++ ++ ++ +++ C. therm. Polymerase for RT in Two-step RT-PCRh up to 10kb specific, oligo(dT) hexamers 37C + + + n.d. + + M-MuLV Reverse Transcriptase up to 12kb specific, oligo(dT)

hexamers 42C (up to 60C) ++

++ ++

++ +

+ +

+ +

+ +

+ AMV Reverse Transcriptase First Strand cDNA Synthesis Kit for RT-PCR (AMV) up to 14kb specific, oligo(dT) hexamers 42C (up to 50C) ++ ++ + + + + Expand Rverse Transcriptasei a For more information on preparing total RNA, mRNA, and viral RNA, please visit our Nucleic Acid Isolation and Purification Special Interest Site b One-step RT-PCR offers faster reactions, reduced risk of contamination, and improved sensitivity. c For prevention of carry-over contamination with Uracil-DNA Glycosylase (UNG) during One-Step RT-PCR, we recommend using the C. therm. DNA Polymerase One-Step RT-PCR System for products up to 3 kb. For short products (up to 1 kb), you may also use the Tth DNA Polymerase. However note that Tth DNA Polymerase is partially inhibited when using dUTP/UNG instead of dTTP. d Two-step RT-PCR offers maximum flexibility with respect to choice of primers, enzyme, and temperature. Two-step RT-PCR also allows the product of a single reverse transcription to be used in several PCR reactions (for analysis of multiple transcripts). e Sensitivity was com paratively tested for fragments in the range of 0.13.0 kb. f Reverse transcription was performed with a sequence specific primer which was then also used for the subsequent PCR step. g Each of the products in this column should be used for first-strand cDNA synthesis only (first step). h C. therm. Polymerase yields optimal results with GC-rich RNA templates. i Not for sale in the U.S.


Page: All 1 2 3

Related biology technology :

1. Properties of Reverse Transcriptases
2. Reverse transcriptases suitable for one-step RT-PCRb
3. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
4. Transcriptor Reverse Transcriptase
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Suggested applications for reverse transcriptases
Post Your Comments:

(Date:10/10/2015)... -- Le 8 octobre, l,honorable Doris O. Matsui ... Congrès sa déclaration de soutien à la troisième ... plasma (IPAW), qui se tiendra du 11 au ... Protein Therapeutics Association (PPTA) et ses sociétés ... Sensibiliser l,opinion publique mondiale autour du don de ...
(Date:10/9/2015)...  DePuy Synthes Trauma* announced today the U.S. launch ... Technology**, the only pre-hydrated demineralized cancellous bone tissue matrix ... ankle, hand and wrist), including fusion, and for filling ... 2015 Orthopaedic Trauma Association (OTA) Annual Meeting. ... bone growth) and osteoinductive 2,3 (stimulates new bone ...
(Date:10/8/2015)... BEACH, Fla. , Oct. 8, 2015   ... in synthetic biology, today announced the appointment of ... of Environment Sector, succeeding Nir Nimrodi who ... Mr. Vaillancourt will direct Intrexon,s endeavors to generate sustainable, ... North America , where he held a variety ...
(Date:10/8/2015)... -- Celemics, a global pioneer of personalized medicine genetics, ... with the establishment of Celemics America, Inc. ... , has secured itself in the global market ... preparation for DNA sequencing and genetic testing.  Next Generation ... testing more accurately, quickly, and cost effectively than traditional ...
Breaking Biology Technology:
(Date:10/1/2015)... NEW YORK , Oct. 1, 2015 /PRNewswire/ ... measuring and analysing human body characteristics, such as ... for authentication purposes. Adoption of biometrics technology has ... over the last five years. In addition ... authentication, that is fingerprint recognition, other means of ...
(Date:9/29/2015)... 29, 2015  iDAvatars is excited to be named one ... to market. The official announcement was recently made at an ... in San Francisco , where iDAvatars presented ... IBM Watson. "It is both an honor and ... to bring to market the cognitive power of IBM Watson ...
(Date:9/28/2015)... SAN JOSE, Calif. , Sept. 28, 2015 /PRNewswire/ ... developer of human interface solutions, today announced that Lenovo ... area touch fingerprint sensor, FS4202, for its latest smartphone, ... enables secure, password-free access to unlock the device and ... consumers. The feature-rich Natural ID FS4202 ...
Breaking Biology News(10 mins):