Navigation Links
Reverse transcriptases suitable for two-step RT-PCRd

Product size Type of primers Primer anneal. temp. Sensitivitye Specificityf Fidelity in RT GC-rich structures Product to selectg total RNA viral RNA specific primer oligo dt primer up to 1kb specific 55-70C n.d. n.d. n.d. n.d. - n.d. Tth DNA Polymerase up to 4kb specific, oligo(dT) hexamers 55-70C + + (+) ++ ++ ++ +++ C. therm. Polymerase for RT in Two-step RT-PCRh up to 10kb specific, oligo(dT) hexamers 37C + + + n.d. + + M-MuLV Reverse Transcriptase up to 12kb specific, oligo(dT)

hexamers 42C (up to 60C) ++


++ ++


++ +


+ +


+ +


+ +


+ AMV Reverse Transcriptase First Strand cDNA Synthesis Kit for RT-PCR (AMV) up to 14kb specific, oligo(dT) hexamers 42C (up to 50C) ++ ++ + + + + Expand Rverse Transcriptasei a For more information on preparing total RNA, mRNA, and viral RNA, please visit our Nucleic Acid Isolation and Purification Special Interest Site b One-step RT-PCR offers faster reactions, reduced risk of contamination, and improved sensitivity. c For prevention of carry-over contamination with Uracil-DNA Glycosylase (UNG) during One-Step RT-PCR, we recommend using the C. therm. DNA Polymerase One-Step RT-PCR System for products up to 3 kb. For short products (up to 1 kb), you may also use the Tth DNA Polymerase. However note that Tth DNA Polymerase is partially inhibited when using dUTP/UNG instead of dTTP. d Two-step RT-PCR offers maximum flexibility with respect to choice of primers, enzyme, and temperature. Two-step RT-PCR also allows the product of a single reverse transcription to be used in several PCR reactions (for analysis of multiple transcripts). e Sensitivity was com paratively tested for fragments in the range of 0.13.0 kb. f Reverse transcription was performed with a sequence specific primer which was then also used for the subsequent PCR step. g Each of the products in this column should be used for first-strand cDNA synthesis only (first step). h C. therm. Polymerase yields optimal results with GC-rich RNA templates. i Not for sale in the U.S.
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Properties of Reverse Transcriptases
2. Reverse transcriptases suitable for one-step RT-PCRb
3. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
4. Transcriptor Reverse Transcriptase
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Suggested applications for reverse transcriptases
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/26/2017)... ... May 25, 2017 , ... ... full process behind each occurrence. Live cell imaging using fluorescence microscopy is the ... use of automated fluorescence microscopy methods will be discussed, from small animal models ...
(Date:5/24/2017)... ... May 24, 2017 , ... Today, the South Davis ... Advanced Biological Nutrient Recovery (ABNRâ„¢) technology at its 4,000,000 gallon per day South ... to sustainably meet current and future nutrient discharge regulations. The ABNR platform, which ...
(Date:5/24/2017)... 2017 (PRWEB) , ... May 24, 2017 , ... ... Medical systems are increasingly being developed with Wi-Fi connectivity to reduce the amount ... from room to room. In addition, compact mobile devices including infusion pumps, heart ...
(Date:5/23/2017)... Westminster, Colorado (PRWEB) , ... May 23, 2017 , ... ... amaranth as the most troublesome and difficult to control weed in 12 categories of ... found. , Almost 200 weed scientists across the U.S. and Canada participated in the ...
Breaking Biology Technology:
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market ... Authorization), Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast ... from USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, ... ... MarketsandMarkets Logo ...
(Date:4/11/2017)... 11, 2017 No two people are ... the New York University Tandon School of Engineering ... found that partial similarities between prints are common ... mobile phones and other electronic devices can be ... vulnerability lies in the fact that fingerprint-based authentication ...
(Date:4/5/2017)... 2017  The Allen Institute for Cell Science today ... one-of-a-kind portal and dynamic digital window into the human ... first application of deep learning to create predictive models ... and a growing suite of powerful tools. The Allen ... future publicly available resources created and shared by the ...
Breaking Biology News(10 mins):