Navigation Links
Reverse transcriptases suitable for two-step RT-PCRd

Product size Type of primers Primer anneal. temp. Sensitivitye Specificityf Fidelity in RT GC-rich structures Product to selectg total RNA viral RNA specific primer oligo dt primer up to 1kb specific 55-70C n.d. n.d. n.d. n.d. - n.d. Tth DNA Polymerase up to 4kb specific, oligo(dT) hexamers 55-70C + + (+) ++ ++ ++ +++ C. therm. Polymerase for RT in Two-step RT-PCRh up to 10kb specific, oligo(dT) hexamers 37C + + + n.d. + + M-MuLV Reverse Transcriptase up to 12kb specific, oligo(dT)

hexamers 42C (up to 60C) ++


++ ++


++ +


+ +


+ +


+ +


+ AMV Reverse Transcriptase First Strand cDNA Synthesis Kit for RT-PCR (AMV) up to 14kb specific, oligo(dT) hexamers 42C (up to 50C) ++ ++ + + + + Expand Rverse Transcriptasei a For more information on preparing total RNA, mRNA, and viral RNA, please visit our Nucleic Acid Isolation and Purification Special Interest Site b One-step RT-PCR offers faster reactions, reduced risk of contamination, and improved sensitivity. c For prevention of carry-over contamination with Uracil-DNA Glycosylase (UNG) during One-Step RT-PCR, we recommend using the C. therm. DNA Polymerase One-Step RT-PCR System for products up to 3 kb. For short products (up to 1 kb), you may also use the Tth DNA Polymerase. However note that Tth DNA Polymerase is partially inhibited when using dUTP/UNG instead of dTTP. d Two-step RT-PCR offers maximum flexibility with respect to choice of primers, enzyme, and temperature. Two-step RT-PCR also allows the product of a single reverse transcription to be used in several PCR reactions (for analysis of multiple transcripts). e Sensitivity was com paratively tested for fragments in the range of 0.13.0 kb. f Reverse transcription was performed with a sequence specific primer which was then also used for the subsequent PCR step. g Each of the products in this column should be used for first-strand cDNA synthesis only (first step). h C. therm. Polymerase yields optimal results with GC-rich RNA templates. i Not for sale in the U.S.
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Properties of Reverse Transcriptases
2. Reverse transcriptases suitable for one-step RT-PCRb
3. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
4. Transcriptor Reverse Transcriptase
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Suggested applications for reverse transcriptases
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/10/2016)... , Feb. 10, 2016  The Maryland House of ... has announced that University of Maryland School of Medicine ... and University of Maryland Medical System President and CEO ... "Speaker,s Medallion," the highest honor given to the public ... Dean Reece and Mr. Chrencik for ...
(Date:2/10/2016)...  IsoRay, Inc. (NYSE MKT: ISR), a medical technology ... applications for the treatment of prostate, brain, lung, head ... results for the second quarter and six months of ... --> --> Revenue was ... which ended December 31, 2015, a 12% increase compared ...
(Date:2/10/2016)... ... February 10, 2016 , ... HOLLOWAY AMERICA, ... Pharmaceutical Engineering (ISPE) Rocky Mountain Chapter 21st Annual Vendor Exhibition on Thursday, February ... than 100 tables for its annual event, which will run from 3:00 p.m. ...
(Date:2/10/2016)... ... February 10, 2016 , ... Curoverse announced today ... Azure, Arvados provides capabilities for managing and processing genomic and health data at ... major institutions collecting and analyzing genomic data,” said Adam Berrey chief executive officer ...
Breaking Biology Technology:
(Date:2/11/2016)... Feb. 11, 2016  Vigilant Solutions announces today that its ... being used by Lee,s Summit Police ... location and arrest of a homicide suspect. ... covers around 65 square miles and is home to roughly ... has a single mobile license plate reader system and also ...
(Date:2/10/2016)... February 10, 2016 ... to 2016 iris recognition market report, combined ... is more widely accepted for border control. ... fingerprint and iris recognition technology in a ... avoid purchasing two individual biometrics devices. ...
(Date:2/9/2016)... AWRE ), a leading supplier of biometrics software and services, ... December 31, 2015.  --> --> ... an increase of 61% compared to $4.3 million in the same ... was $2.6 million compared to $0.2 million in the fourth quarter ... Higher revenue and operating income in the fourth quarter of this ...
Breaking Biology News(10 mins):