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Reverse transcriptases suitable for one-step RT-PCRb

Product size Type of primers Primer anneal. temp. Sensitivity Specifi-
city
Fidelity in PCR Tolerance
of non-
specific RNA
Tolerance
of serum components
Carry-over prevention dUTP/UNGc Product to select mRNA Viral RNA/RNA rich in secondary structure up to 1kb specific 55-70C + + + - + + + Tth DNA Polymerase up to 3kb specific 55-70C ++ ++ ++ + +++ ++ + C. therm. Polymerase One-step RT-PCR Systemh up to 6kb specific 45-60C +++ + + ++ ++ + not suitable Titan One Tube RT-PCR System/Kit a For more information on preparing total RNA, mRNA, and viral RNA, please visit our Nucleic Acid Isolation and Purification Special In
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Related biology technology :

1. Properties of Reverse Transcriptases
2. Reverse transcriptases suitable for two-step RT-PCRd
3. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
4. Transcriptor Reverse Transcriptase
5. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
6. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
7. Recombinant HIV Reverse Transcriptase
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Suggested applications for reverse transcriptases
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Acetylcholinesterase from human and monkey....
...
... Dye runs faster than Bromophenol blue,or ... gels.,Orange G dye migrates with DNA ... migration at this size,prevents the obscuring ... the front of DNA migration.,The Orange ...
... solution, Type I is ... and agarose gel electrophoresis ... offers the advantage of ... blue and xylene cyanole). ...
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