( ...)Reverse transcriptases suitable for one-step RT-PCRb

Product size Type of primers Primer anneal. temp. Sensitivity Specifi-
city Fidelity in PCR Tolerance
of non-
specific RNA Tolerance
of serum components Carry-over prevention dUTP/UNG^c Product to select mRNA Viral RNA/RNA rich in secondary structure up to 1kb specific 55-70C + + + - + + + Tth DNA Polymerase up to 3kb specific 55-70C ++ ++ ++ + +++ ++ + C. therm. Polymerase One-step RT-PCR System^h up to 6kb specific 45-60C +++ + + ++ ++ + not suitable Titan One Tube RT-PCR System/Kit a For more information on preparing total RNA, mRNA, and viral RNA, please visit our Nucleic Acid Isolation and Purification Special In
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