Navigation Links
Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal,,,Performance

esentative sample from each packaging of a restriction endonuclease is confirmed prior to shipment to demonstrate that no loss of enzymatic activity occurs upon packaging.

Overdigestion of Substrates

When enzymes are tested under standard digestion conditions (1 U of enzyme/g substrate DNA), trace levels of contaminating DNase activities might not be detected. To more stringently assess the functional purity of each enzyme preparation, Stratagene incubates a 4- to 100-fold excess of enzyme with substrate DNA. After a 16-hour incubation at the appropriate temperature, the banding pattern from these overdigested samples is compared to the signature-banding pattern produced by the enzyme in 1 hour. If the restriction endonuclease preparation is free from contaminating endonuclease and exonuclease activities, the overdigested reactions will exhibit the identical banding pattern characteristic of the 1-hour reactions. The maximum number of units that clearly exhibit this pattern in the overdigestion reactions is reported on the Certificate of Analysis provided with each enzyme. This overdigestion assay is also employed on a representative sample following aliquoting and packaging to confirm that contaminating DNase activity is not introduced during packaging.

Detecting Exonuclease Contaminants

The integrity of a restriction enzyme cut site can be destroyed by the presence of exonucleases that can digest single-or double-stranded ends. Stratagene specifically assays for exonuclease activity by incubating an excess of restriction endonuclease for 4 hours with a mixture of single- and double-stranded, [3H]-thymidine-labeled genomic E. coli DNA. Contaminating exonuclease is indicated if over 0.6% of the 3H-labeled DNA added to the reactions is detected as trichloroacetic acid-soluble counts.

Ligation Assay

Figure 1'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
3. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
4. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
5. Sciclone ALH Bulk Dispense Precision Testing
6. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
7. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
8. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
9. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
10. Testing Compressed Air Lines for Microbiological Contamination
11. Testing Compressed Air Lines for Microbiological Contamination
Post Your Comments:
(Date:11/26/2014)... November 26, 2014 On January, 14, ... Baltimore and Washington D.C. science community to the UMBC ... event, sponsored by the Protein Society, will take place ... Center, Room 312. Through discussion, presentations and workshops, ... importance of proteins, their role in the industry and ...
(Date:11/26/2014)... NJ (PRWEB) November 25, 2014 ... has officially joined the Morris County (New Jersey) ... which is a leading business organization in the ... opportunity to further engage some key clients in ... operations in the geographic area. Membership enables Whitehouse ...
(Date:11/26/2014)... Continuing its award-winning streak, Nerium International, the leader ... for outstanding creative achievement by its marketing and communication ... organization’s top honor, in the Branding category for its ... of this year. The event showcased top speakers and ... awarded a Gold MarCom Award in the Special Events ...
(Date:11/26/2014)... November 25, 2014 Miles Holder, formerly ... the Graphel Carbon Products team as Sales/Marketing Manager. Mr. ... and customer service for the graphite industry. , “We ... our team,” stated Dave Trinkley, VP of Market and ... range of experience in the graphite industry, along with ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2Miles Holder Joins Graphel Corporation as Sales/Marketing Manager 2
... required regulatory approvals for the $2.3 billion acquisition of ... divest of two divisions of the company they are ... manufacturer plans to integrate Instrumentarium it into its ... CEO, Dow Wilson.. , ,The final required ...
... to open a new state-of-art healthcare facility in October, ... latest in digital imaging technology from technology vendor Emageon. ... and distribution of digital medical images across its integrated ... in 1984 is a nationally recognized leader in efforts ...
... Internet connection? , ,MADISON, WI - McDonalds ... connections in five Milwaukee locations as part of a ... restaurants in the San Francisco Bay Area, Metropolitan New ... McDonald's Wireless Internet service. , ,Customers can purchase an ...
Cached Biology Technology:Aurora Selects New Imaging Technology 2Wisconsin McDonalds Go Wireless 2
(Date:11/18/2014)... 17, 2014 The Parenteral Drug Association (PDA) today ... will speak and at least seven more will participate in ... Omni Shoreham Hotel in Washington D.C. , ... have significant support from the regulatory agencies in ... in our effort to help advance the use of ...
(Date:11/10/2014)... detergent pods began appearing on U.S. store shelves in ... numbers ever since. The small packets can be tossed ... out a liquid or powder. The convenience, though, has ... study from researchers at Nationwide Children,s Hospital found that ... reports of 17,230 children younger than 6 years of ...
(Date:11/6/2014)... deaths occur because of metastasis, yet progress in ... slow. , "It,s been particularly challenging to design ... research fellow in systems biology at Harvard Medical ... until after they,ve already metastasized." , Gujral and ... should help researchers better understand how metastasis begins. ...
Breaking Biology News(10 mins):FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Study finds laundry detergent pods, serious poisoning risk for children 2Migration negation 2Migration negation 3
... medical woes ranging from cancer to bird flu will ... The newly-patented device will offer small and medium-sized researchers ... It will also allow them a chance, at last, ... analyze drugs and view the characteristics of protein. ...
... a gene known to increase the risk of Alzheimer,s ... Oregon Health & Science University study has found. ... that 7- to 10-year-olds with a member of a ... and neuroprotection display reduced spatial learning and memory, associated ...
... include: continental deformation and the San Andreas fault; ... between Britain and America; environmental stress and the ... Canada,s Mackenzie River basin as a CO2 source ... the absence of active volcanism; and geologic evolution ...
Cached Biology News:Council to honor NJIT SmartPin 2Children with gene show reduced cognitive function 2November GEOLOGY and GSA TODAY Media Highlights 2November GEOLOGY and GSA TODAY Media Highlights 3November GEOLOGY and GSA TODAY Media Highlights 4November GEOLOGY and GSA TODAY Media Highlights 5November GEOLOGY and GSA TODAY Media Highlights 6November GEOLOGY and GSA TODAY Media Highlights 7November GEOLOGY and GSA TODAY Media Highlights 8November GEOLOGY and GSA TODAY Media Highlights 9November GEOLOGY and GSA TODAY Media Highlights 10