Navigation Links
Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal,,,Performance


Reliable restriction enzymes satisfy all your research needs

Ronda Allen Kim Kuplent
Stratagene

Restriction endonucleases are used in many molecular biology protocols, making the functional purity of critical importance. Each restriction enzyme preparation must be free of contaminants that can potentially interfere with either DNA cleavage or subsequent reactions that use digested DNA (i.e., ligation or sequencing reactions). Such contaminants might include other site-specific endonucleases that produce unwanted DNA fragments, exonucleases that destroy the integrity of the cut site by cleaving nucleotides from the resultant 5 or 3 termini of the fragments, and phosphatases that inhibit ligation reactions by removing the 5 phosphate from DNA ends. To ensure that Stratagenes enzymes consistently meet the needs of the research community, we impose strict quality control procedures in analyzing every enzyme preparation. In most instances, the testing conditions are designed to be more rigorous than typical laboratory applications. In this article, we describe the assays used to establish the functional purity of restriction endonuclease preparations.

Unit Activity Determination

Prior to assaying samples for contaminants, the unit activity of each enzyme sample is accurately determined. One unit of activity is defined as the amount of enzyme required to completely digest 1 g of substrate DNA in 1 hour at the appropriate temperature under the optimal assay conditions. This information is provided on the Certificate of Analysis provided with each restriction endonuclease. Restriction enzyme activity is substrate-dependent; therefore, when digesting a new substrate, we recommend titrating the amount of enzyme added. The unit activity of a repr
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
3. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
4. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
5. Sciclone ALH Bulk Dispense Precision Testing
6. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
7. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
8. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
9. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
10. Testing Compressed Air Lines for Microbiological Contamination
11. Testing Compressed Air Lines for Microbiological Contamination
Post Your Comments:
(Date:10/25/2014)... October 25, 2014 Berkshire Corporation, ... pleased to announce the release of the ValuSeal® ... level of contamination containment in critical processing applications. ... and higher cleanroom environments, ValuSeal® IonX™ is a ... laundered wiper that continues Berkshire’s innovative focus on ...
(Date:10/25/2014)...  RegeneRx Biopharmaceuticals, Inc. (OTCBB: RGRX) ("the Company" or ... International Symposium on Thymosins in Health and Disease in ... "When thymosin beta 4 (TB4) linked with rhodamine (a ... in a rodent model of stroke, it was visualized ... (the functional tissue of the brain).   The leakage of ...
(Date:10/25/2014)... DUBLIN , October 24, 2014 /PRNewswire/ ... and Markets ( http://www.researchandmarkets.com/research/t5t5pd/the_global_market ) has announced the ... Carbon Nanotubes, Graphene And Other 2-D Nanomaterials ...      (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , ,CNTs and graphene ... fibres known to man, with a performance-per-weight ...
(Date:10/22/2014)... , Oct. 22, 2014  Actavis plc (NYSE: ... company, and Rhythm, a biopharmaceutical company, today announced that ... Rhythm,s wholly owned subsidiary, Rhythm Health, Inc., which is ... treatment of diabetic gastroparesis and other GI functional disorders. ... 2 trial of relamorelin for the treatment of diabetic ...
Breaking Biology Technology:Ions Are Bygones, Berkshire Announces the Release of New Innovative Cleanroom Wipers 2Researchers Report Thymosin Beta 4 Crosses Blood-Brain Barrier In Animal Stroke Model 2The Global Market for Carbon Nanotubes, Graphene and Other 2-D Nanomaterials 2The Global Market for Carbon Nanotubes, Graphene and Other 2-D Nanomaterials 3The Global Market for Carbon Nanotubes, Graphene and Other 2-D Nanomaterials 4Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 2Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 3Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 4Actavis Signs Option to Acquire Rhythm Subsidiary and its GI Development Product, Relamorelin 5
... May 24, 2010 Simbionix USA Corporation,the world,s leading provider of ... is launching,two new hands-on training systems- the Lap Nepherctomy simulation module and,the ... AUA annual meeting in San Francisco next week. , ... The Lap ...
... BEIJING , May 24 /PRNewswire-Asia/ -- Sinovac Biotech Ltd. (Nasdaq: ... Weidong Yin ,Chairman, President & CEO, will present at the 2010 UBS Global ... London,England . Sinovac,s presentation is scheduled for Wednesday, June 2, 2010 ... , , ...
... ... ... plans to add to its natural color capabilities by investing $16 million at its Color ... facility dedicated wholly to natural colors production. The plant will be the largest and most ...
Cached Biology Technology:Simbionix to Reveal Two new Products at the Upcoming American Urology Association (AUA) Annual Meeting 2Simbionix to Reveal Two new Products at the Upcoming American Urology Association (AUA) Annual Meeting 3Sinovac to Present at 2010 UBS Global Specialty Pharmaceuticals Conference 2Sensient Technologies Corporation Announces Major Investment in Natural Colors 2
(Date:10/25/2014)... 2014 Research and Markets  has announced ... 2014-2018" report to their offering. ... on the basis of their physical or behavioral patterns. ... traits such as fingerprints, face recognition, DNA, palm print, ... as keystroke patterns and speech pattern/voice recognition. ...
(Date:10/22/2014)... The Nano-Bio Manufacturing Consortium (NBMC), an industry-academia ... (AFRL), has chosen a project proposed by the Arizona ... Arizona College of Medicine – Tucson ... is to assess different sweat collection methods and devices ... under a variety of human-body conditions, the results of ...
(Date:10/22/2014)... 21, 2014 Aware, Inc. (NASDAQ: AWRE ... reported financial results for its third quarter ended September 30, ... was $6.0 million, an increase of 40% compared to $4.3 ... the third quarter of 2014 was $4.1 million compared to ... income in the current three month period was primarily due ...
Breaking Biology News(10 mins):Biometrics Market in Europe 2014-2018: Key Vendors are 3M Cogent, Cognitec, NEC and Safran 2Biometrics Market in Europe 2014-2018: Key Vendors are 3M Cogent, Cognitec, NEC and Safran 3Nano-Bio Manufacturing Consortium Selects Project Proposed by Arizona Center for Integrative Medicine to Optimize Human Performance Monitoring Techniques 2Nano-Bio Manufacturing Consortium Selects Project Proposed by Arizona Center for Integrative Medicine to Optimize Human Performance Monitoring Techniques 3Aware, Inc. Reports Third Quarter 2014 Financial Results 2Aware, Inc. Reports Third Quarter 2014 Financial Results 3Aware, Inc. Reports Third Quarter 2014 Financial Results 4Aware, Inc. Reports Third Quarter 2014 Financial Results 5Aware, Inc. Reports Third Quarter 2014 Financial Results 6Aware, Inc. Reports Third Quarter 2014 Financial Results 7
... hold great promise for benefiting degenerative diseases, and do ... grown in a manner compatible with clinical use (i.e., ... for immunosuppression. These were a few of a number ... by Evan Y. Snyder, M.D., Ph.D., and spearheaded by ...
... from another may depend on the number of receptors ... of receptors is controlled by levels of a ubiquitous ... found. , Everything from the ability to concentrate, perceive ... sclerosis, muscular dystrophy, post-traumatic stress syndrome and schizophrenia is ...
... launch of the first large-scale study to evaluate a ... today by study collaborators in the United States and ... participants at five sites throughout South Africa and is ... are part of the HIV Vaccine Trials Network (HVTN), ...
Cached Biology News:Stem cells act through multiple mechanisms to benefit mice with neurodegenerative disease 2Stem cells act through multiple mechanisms to benefit mice with neurodegenerative disease 3Stem cells act through multiple mechanisms to benefit mice with neurodegenerative disease 4Stem cells act through multiple mechanisms to benefit mice with neurodegenerative disease 5Bisexual fruit flies show new role for neurochemical 2Africa's first large-scale HIV vaccine study launches 2Africa's first large-scale HIV vaccine study launches 3Africa's first large-scale HIV vaccine study launches 4
HybriWell™ hybridization sealing system, 20 mm diameter chamber, 0.15 mm deep *set of 100*...
Recombinant Feline IL-5...
... Checker Kits provide two highly efficient methods ... time-consuming plasmid preparation. By using the large-scale ... need to do is to transfer partial ... the transformation plates into the solutions provided ...
Recombinant Equine IL-4, CF...
Biology Products: