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Research Tools for Analysis of ZAP-70

cells were run and compared to a standard curve. As can be seen (Figure 1), some of the patients had very high levels (>1000 MFI, 400-800 units), others had intermediate levels (several hundred, 150-400 units), and others had less than 50 MFI (0-70 units). Our results correlated with Dr. Kipps results using other methods (western blots, flow cytometry) to determine which donor cells expressed ZAP-70.

Please visit the following websites for additional product information:

bdbiosciences/flexset.com
for BD CBA Flex Set product details

bdbiosciences/bdimag.com
for BD IMag cell separation reagent product details

References

1. Palacios, E.H. and Weiss, A. (2004) Function of the Src-family kinases, Lck and Fyn, in T-cell development and activation. Oncogene 23:7990-8000.

2. Abraham, R.T. and Weiss, A. (2004) Jurkat T cells and development of the T-cell receptor signalling paradigm. Nature Reviews Immunol. 4:301-308.

3. DalPorto, J.M. et al. (2004) B cell antigen receptor signaling 101. Mol. Immunol. 41:599-613.

4. Wiestner, A. et al. (2003) ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood 101:4944-4951.

5. Rassenti, L.Z. et al. (2004) ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. New Engl. J. Med. 351-893-901.

6. Chen, L. et al (2005) ZAP-70 directly enhances IgM signaling in chronic lymphocytic leukemia, Blood 105:2036-2041.


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