Navigation Links
Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

oducible. The kit contains a detailed fixing protocol, fixing reagents, and a shipping container that holds up to 50 samples; the price includes analysis for 50 samples performed at Litron using their patented FCM-based method.

After sending blood samples for analysis, a minimum of 10,000 RETs per blood sample are examined for MN, compared to 1,000 to 2,000 using microscopic-scoring techniques. Concurrently, micronuclei are scored in the total RBC population, representing an index of subchronic/chronic treatments. The frequency of RETs is determined as an indication of stem cell toxicity. Results are returned in the form of a GLP report within seven days from receipt of the samples.

Conclusions

The MN endpoint continues to grow in popularity as a genotoxic screen to identify chromosome-damaging agents. Compared to conventional MN methods, the icroFlow Micronucleus Analysis Kit offers more MN data in less time and at a lower cost and with higher precision. When used in conjunction with Stratagenes Big Blue Transgenic Rodent Mutagenesis Assay, a test compound or treatment can be assessed for both mutagenic and clastogenic/aneugenic potential within the same animal.

REFERENCES
  1. Matter, B. and Schmid, W. (1971) Mutation Res. 12: 417-425.

  2. Heddle, J. (1973) Mutation Res. 18: 187-190.

  3. MacGregor, J., et al. (1983) In A.W. Hayes, R.C. Schnell and T.S. Miya (Eds.), Developments in the Science and Practice of Toxicology, pp. 555-558. Elsevier, Amsterdam.

  4. Maier, P. and Schmid, W. (1976) Mutation Res. 40: 325-338.

  5. Hayashi, M., Sofuni, T., and Ishidate, M. (1984) Mutation Res.141: 165-169.

  6. Process for Analyzing Clastogenic Agents. Pat
    '"/>



Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
2. Efficient and Reliable Linear Amplification of cRNA
3. Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool
4. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
5. Fast, Accurate Assessment of siRNA-induced Gene Silencing
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
8. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Sciclone ALH Bulk Dispense Precision Testing
11. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
Post Your Comments:
(Date:9/23/2014)... The Minnesota High Tech Association ... cellular modems , platforms and application-specific remote monitoring ... for the 2014 Tekne Awards. The awards ... Convention Center on Thursday, November 13th. The Tekne ... shown superior technology innovation and leadership. , ...
(Date:9/22/2014)... Pearl's Premium Ultra Low Maintenance Lawn Seed ... seed this week with the release of their Pearl's ... the fact that it is time to plant now ... , As of the date 6/4/13, Pearl's Premium had ... experts and the media including: The National Gardeners ...
(Date:9/22/2014)... Thanks to new LEGO-like components developed by researchers ... is now possible to build a 3-D microfluidic ... small modules by hand. , Microfluidic systems ... and biotechnology to precisely manipulate small volumes of ... or DNA analysis, pathogen detection, clinical diagnostic testing, ...
(Date:9/22/2014)... a new star-shaped molecule made up of interlocking rings, which ... , Known as a ,Star of David, molecule, scientists ... of a century and the team,s findings are published in ... two molecular triangles, entwined about each other three times into ... triangle is 114 atoms in length around the perimeter. The ...
Breaking Biology Technology:NimbeLink Named Finalist for 15th Annual Tekne Awards 2NimbeLink Named Finalist for 15th Annual Tekne Awards 3Pearl's Premium Ultra Low Maintenance Lawn Seed Announces A Major Breakthrough In Drought Tolerant Grass Seed 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 3Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 4Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 5
... Finger Nuclease Platform enables Precise, Permanent, and ... Heritable Modification of the Genome, ... CompoZr(TM) platform of zinc finger nuclease (ZFN),reagents. The CompoZr ZFN ... to target and precisely manipulate the genome,of living cells, resulting ...
... plant-based extract, is better than metformin, the leading Type 2 diabetes ... ... Winter Haven, FL (PRWEB) September 16, 2008 -- A ... 2 diabetics better than a leading prescription drug, according to the ...
... Development of Gene Expression Signatures Derived by COXEN ... Molecular Test of Responsiveness to Current Treatments, ... Transgenomic (OTC Bulletin Board: TBIO) today announced a ... test which will guide,clinicians in their choice of ...
Cached Biology Technology:Sigma-Aldrich Launches Breakthrough Genome-Editing Tools 2Sigma-Aldrich Launches Breakthrough Genome-Editing Tools 3Human Trial Results Show Plant Extract Tops Drug at Regulating Blood Sugar Levels; Huge Market Seen for Newly Developed Food Additive Emulin™ 2Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options 2Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options 3Transgenomic Announces Partnership With Key Genomics for Ovarian Cancer Assay to Guide Therapeutic Options 4
(Date:9/21/2014)... (black-eyed peas) is a fascinating example of how science ... the story of the scientist behind the research. ... the 21st Century to serve as an accessible summary ... recently published by the Crop Science Society of America, ... , Singh has devoted his life,s work to solving ...
(Date:9/21/2014)... MA -- In recent years, new strains of bacteria ... Each year, these superbugs, including drug-resistant forms of tuberculosis ... and kill at least 23,000. Despite the urgent need ... classes of antibiotics in the past decade. , MIT ... these superbugs. Using a gene-editing system that can disable ...
(Date:9/21/2014)... a protein therapy that disrupts the process that causes ... travel through the blood stream and start aggressive new ... as metastasis, can cause cancer to spread with deadly ... to cancer fall prey to metastatic forms of the ... who describes a new therapeutic approach in Nature ...
Breaking Biology News(10 mins):Battling superbugs 2Battling superbugs 3Battling superbugs 4Stanford researchers create 'evolved' protein that may stop cancer from spreading 2Stanford researchers create 'evolved' protein that may stop cancer from spreading 3Stanford researchers create 'evolved' protein that may stop cancer from spreading 4
... long puzzled scientists, and several scenarios have been proposed ... group appeared virtually simultaneously on Asia, Europe and North ... new research using the latest evidence suggests a completely ... that sudden, rapid global warming drove the dispersal. ...
... Researchers at the Burnham Institute for Medical Research have ... free radicals and accumulation of misfolded proteins that lead ... such as Alzheimer's and Parkinson's Disease. , ... protein that is necessary for proper protein folding in ...
... The study of 18 HIV-positive subjects shows that HIV in ... HIV that lives in the blood and peripheral tissues. , ... with the presence of a particular mutation in the HIV ... 2006 issue of Brain. It was led by Satish K. ...
Cached Biology News:Ancient global warming drove early primates' dispersal 2Key stress protein linked to toxicities responsible for Parkinson's, Alzheimer's 2Viral genetic differences are possible key to HIV dementia 2Viral genetic differences are possible key to HIV dementia 3
... is proud to work in collaboration with ... 25mm syringe filters in a variety of ... licensed for use on the Caliper TPWII, ... and Pricing for our Automation Certified Syringe ...
... 3 Dodeca cell with multi-casting chamber is used ... 12 mini format handcast (8.3 x 7.3 cm) ... cell requires 3.4-4.4 L of buffer and includes ... attaches to an external refrigerated circulator (not included), ...
...
... This product has been developed to ... peroxidase labeled proteins. Stability is increased ... to 2-8 centigrade. This product is ... no mercury or azide. This formulation ...
Biology Products: