Navigation Links
Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

oducible. The kit contains a detailed fixing protocol, fixing reagents, and a shipping container that holds up to 50 samples; the price includes analysis for 50 samples performed at Litron using their patented FCM-based method.

After sending blood samples for analysis, a minimum of 10,000 RETs per blood sample are examined for MN, compared to 1,000 to 2,000 using microscopic-scoring techniques. Concurrently, micronuclei are scored in the total RBC population, representing an index of subchronic/chronic treatments. The frequency of RETs is determined as an indication of stem cell toxicity. Results are returned in the form of a GLP report within seven days from receipt of the samples.

Conclusions

The MN endpoint continues to grow in popularity as a genotoxic screen to identify chromosome-damaging agents. Compared to conventional MN methods, the icroFlow Micronucleus Analysis Kit offers more MN data in less time and at a lower cost and with higher precision. When used in conjunction with Stratagenes Big Blue Transgenic Rodent Mutagenesis Assay, a test compound or treatment can be assessed for both mutagenic and clastogenic/aneugenic potential within the same animal.

REFERENCES
  1. Matter, B. and Schmid, W. (1971) Mutation Res. 12: 417-425.

  2. Heddle, J. (1973) Mutation Res. 18: 187-190.

  3. MacGregor, J., et al. (1983) In A.W. Hayes, R.C. Schnell and T.S. Miya (Eds.), Developments in the Science and Practice of Toxicology, pp. 555-558. Elsevier, Amsterdam.

  4. Maier, P. and Schmid, W. (1976) Mutation Res. 40: 325-338.

  5. Hayashi, M., Sofuni, T., and Ishidate, M. (1984) Mutation Res.141: 165-169.

  6. Process for Analyzing Clastogenic Agents. Pat
    '"/>



Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
2. Efficient and Reliable Linear Amplification of cRNA
3. Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool
4. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
5. Fast, Accurate Assessment of siRNA-induced Gene Silencing
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
8. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. Sciclone ALH Bulk Dispense Precision Testing
11. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
Post Your Comments:
(Date:7/10/2014)... 2014 Women in the West ... an additional family planning option. Sayana® Press has ... all levels of the health system and in ... widely used contraceptive—Pfizer’s Depo-Provera® (depot medroxyprogesterone acetate)—with the ... the first of four African countries expected to ...
(Date:7/10/2014)... UK (PRWEB) July 10, 2014 ... 21st Century Medicine Forum on ‘Commercialising Longevity Research’ ... and entrepreneurs to the London Bioscience Innovation Center ... Research Foundation and Aging Analytics Ltd. The meeting ... investment in translational research for age-related disease, as ...
(Date:7/10/2014)... 2014 Terascala, the industry leader in ... Swahn, a former vice president of product management for ... president of marketing. Terascala’s software when combined with leading ... the highest performance and most reliable solutions for processing ... Terascala’s channel expansion and broaden its product portfolio. His ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Robert Harman, DVM, ... Medicine company, is proud to announce the relaunch of his ... with a new series called “ What are Stem Cells ... an honest and straightforward foundation in the basics of stem ... decision on the right type of treatment when considering regenerative ...
Breaking Biology Technology:Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Terascala Expands Executive Team 2Terascala Expands Executive Team 3Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3
... Terrorist organizations sometimes have an advantage in the media. ... University of Haifa, published in the journal of Israel,s ... the advantage in this arena too. , ... against terrorist organizations, sometimes more so than conventional weapons. ...
... to strengthen its European business OFFENBACH MAIN, Germany, May ... Ltd., a leading multinational full service Contract Research Organization ... as President Strategic Operations Europe. Mr. Spector will be ... Mediation . Mr. Spector brings to Omega Mediation more ...
... Enfant(TM) Pediatric VEP Vision Testing System, Providing Early Detection ... If your child had vision problems, you,d be able ... Goldman, M.D., pediatrician at Children,s Health Center in Gurnee."Children ... deficits, even those they,ve had since birth," says Dr. ...
Cached Biology Technology:Information warfare in the 21st century: Ideas are sometimes stronger than bombs 2SIRO Group Appoints Scott L. Spector as President, Strategic Operations, for its European Business 2Chicago-Area Pediatricians Offering Unique Test to Detect Vision Problems in Children as Young as Six Months of Age 2
(Date:7/11/2014)... of kidney injury related to the use of hydroxyethyl ... HES molecules, according to a report in Anesthesia ... Research Society (IARS). , The "total mass of HES ... human renal proximal tubule cells (PTCs), concludes the laboratory ... Hospital Wrzburg, Austria. Other factorssuch as differences in ...
(Date:7/11/2014)... to the hippocampus, the marginal division of the ... What is the impact degree of substance P ... memory function? Yan Yu and his team, College ... using immunofluorescence staining, that substance P receptor, neurokinin ... striatal marginal division of normal rats. Unilateral or ...
(Date:7/11/2014)... Evidence exists that cannabinoid receptor type 1 ... Ca2+ influx, and reduce neurotransmitter release. However, ... 1 can increase extracellular Ca2+ influx and ... his team, Tongji Hospital Affiliated to Tongji ... Technology, China used whole cell voltage-clamp and ...
Breaking Biology News(10 mins):In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... 2009 The Center for Stem Cell & Regenerative Medicine ... Clinic (CC), University Hospitals (UH), and Athersys, Inc. has ... the Research Commercialization Program. The funding will help support ... four emerging and three pilot projects. This funding will ...
... scientists from Europe and China have gathered in Barcelona this ... research projects using data from ESA and Chinese Earth observation ... and the Ministry of Science and Technology (MOST) of China ... monitor and understand environmental phenomena in China. ,The symposium, held ...
... Although every cell in the body carries the genes ... small proportion of stem cells mature into those important immune-system ... Health and his colleagues have identified two "molecular motors" that ... cells. They published their findings June 19 in the online ...
Cached Biology News:CWRU receives $5 million from Ohio Third Frontier Commission 2CWRU receives $5 million from Ohio Third Frontier Commission 3Europe and China watching Earth together 2Pushmi-pullyu of B-cell development discovered 2
...
ANTI DIPHTH TOX A SU...
... shaker flask clips, purpose-designed to firmly retain ... clips are equipped with a top retainer ... a bottom retainer spring, and silicon tubing ... glassware. Clips include the required mounting ...
... An immuncapture enzyme-activity assay based on ... captured on monoclonal antibody coated microtiter plate. ... standards, samples or controls, 2nd ... The assay is intended for the determination ...
Biology Products: