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Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

ytometric methodology, where they are able to count MN in both total red blood cell (RBC) and immature reticulocyte (RET) populations.6,7,8 Their patented method** is based on the observation that RETs express the transferrin receptor (i.e., the CD71-defined antigen), and fluorescent antibodies directed against these surface markers can differentially label immature erythrocytes.9,10 When fixed blood cells are treated with ribonuclease and antitransferrin receptor antibody (FITC-conjugate), and then stained with propidium iodide, normochromatic erythrocytes (NCEs) and RETswith and without MNare clearly resolved (Figure 1).

Figure 1

For researchers who do not have access to flow cytometric equipment or expertise, Stratagene and Litron now provide the icroFlow Micronucleus Analysis Kit to obtain genotoxicity data. The kit assembles all the quality-controlled supplies and reagents, allowing researchers worldwide the ability to collect peripheral blood samples and send then to Litron for FCM micronucleus analysis. Fixing the cells is technically simple (Figure 2) and requires no special equipment. Once the fixed cells are received at Litron, MN frequencies are quickly measured with ease, precision, and sensitivity unmatched by conventional microscopic-scoring techniques.

Figure 2

High-Level Micronuclei Resolution

Figure 3 demonstrates the rel
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