Navigation Links
Reduce Errors and Save Time When Setting Up PCRs

Polymerase chain reaction (PCR) setup typically requires thawing of the various reagents, setting up a master mix, then distributing the mix to the individual reaction tubes.

The various handling steps
  • can cause pipetting mistakes and experimental error
  • bear the risk of contaminating the amplification reaction and
  • are time-consuming.
Facilitate PCR Setup and Obtain Reliable Results with the PCR and High Fidelity PCR Masters

The PCR Master is a 2x concentrated ready-to-use master mix that contains Taq DNA Polymerase, PCR-Grade Deoxynucleotides, and PCR Reaction Buffer with a final concentration of 1.5 mM MgCl2. This mix is designed for the routine amplification of any kind of DNA up to 3 kb.
The High Fidelity PCR Master is a 2x concentrated ready-to-use master mix that contains the Expand High Fidelity Enzyme blend, PCR-Grade Deoxynucleotides, and PCR Reaction Buffer with a final concentration of 1.5 mM MgCl2. The Expand High Fidelity Enzyme blend is a blend of Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This powerful enzyme blend is designed to amplify any kind of DNA up to 5 kb (Figure 1) with higher yield and three times higher fidelity than Taq DNA Polymerase alone.

Advantages of these Master Mixes

  • Save time: Blends are stored at 4C and can be immediately used, no freezing or thawing is necessary.
  • Improve results by reducing pipeting errors: Master mixes contain all the reagents required to perform PCR. Simply add the template DNA, primers, and the required amount of PCR-Grade Water (provided with the products)
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
2. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
3. Setting up the laboratory to avoid contamination
4. Setting up a kinase profiler with IMAP
5. Setting up Successful siRNA Library Screens
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Reduce Errors and Save Time When Setting PCRs

(Date:9/17/2014)... FORT LAUDERDALE, Fla. , Sept. 17, 2014 ... signed an agreement to form a Special Purpose Vehicle in ... based Jatropha centric agro energy company. The SPV shall ... U.S. registered entity with AFAI as majority shareholder. The SPV ... Africa , particularly Ethiopia . ...
(Date:9/17/2014)... RXi Pharmaceuticals Corporation (NASDAQ: ... developing and commercializing innovative therapies addressing major unmet ... has been granted a patent by the United ... delivery system for delivering its "original" RNAi compounds ... treatment of diseases with an inflammatory component. Potential ...
(Date:9/17/2014)... CA (PRWEB) September 17, 2014 Escape Alert, ... for pets which will alert owners if their pet has ... GPS from an implanted microchip in the pet’s body. This ... microchips for pets have been around for many years, they ... are not able to track a pet’s location or alert the ...
(Date:9/17/2014)... (PRWEB) September 17, 2014 The ... committed to lowering the barriers to innovation in ... the appointment of Carmen Nitsche as Executive Director ... to expand its global reach. , Carmen joins ... Vice President, Corporate Development. At Accelrys, she managed ...
Breaking Biology Technology:AFAI Signs Joint Venture Agreement With Jatro Greentech Inc. 2AFAI Signs Joint Venture Agreement With Jatro Greentech Inc. 3RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 2RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 3RXi Pharmaceuticals Strengthens Intellectual Property Portfolio 4Implantable Microchip for Pets with GPS Tracking - Patent Filed by Escape Alert, LLC. 2Implantable Microchip for Pets with GPS Tracking - Patent Filed by Escape Alert, LLC. 3Pistoia Alliance Expands Business Development with Appointment of Carmen Nitsche 2
... photonics and renewable energy will be the focus of ... to 14, 2009 at McMaster University in Hamilton, Ontario, ... in conjunction with the event. , The 4th Nano ... the 14th Canadian Semiconductor Technology Conference (CSTC2009) will bring ...
... UC Riverside have made an accidental discovery in ... information in computers can be transported or stored. ... electrons possess that makes them behave like tiny ... of spin-based semiconductor technology such as ultrahigh-speed computers. ...
... programs give sponsors easy-to-use tools to store, mine and ... transfer knowledge base to ensure enrollment success, NEWTON, ... technology leader, announced the release of a new,product, "Center ... in-house recruitment capabilities for clinical,trial sponsors. The goal is ...
Cached Biology Technology:Joint nanotechnology forum to focus on electronics, photonics, renewable energy 2Discovery by UC Riverside physicists could enable development of faster computers 2Discovery by UC Riverside physicists could enable development of faster computers 3TCN e-Systems Offers Trial Sponsors Solutions for Creating In-House Center of Excellence in Patient Recruitment 2TCN e-Systems Offers Trial Sponsors Solutions for Creating In-House Center of Excellence in Patient Recruitment 3TCN e-Systems Offers Trial Sponsors Solutions for Creating In-House Center of Excellence in Patient Recruitment 4
(Date:9/17/2014)... learned a great deal about complex social behavior by ... something to teach too. , With their unusually large ... parrots live in a complex social environment---not merely in ... or ants, but in a dynamic setting of alliances ... intelligent mammals: dolphins, whales, primates, and social carnivores, like ...
(Date:9/16/2014)... Environmental Health at the Mailman School of Public Health ... asthma and prenatal exposure to two phthalates used in ... in the journal Environmental Health Perspectives . ... higher levels of the chemicals, butylbenzyl phthalate (BBzP) and ... percent increase in risk of developing asthma between age ...
(Date:9/16/2014)... CLEVELAND, Ohio (September 17, 2014)The North American Menopause ... for the comprehensive care of midlife womenon everything ... feature, "The North American Menopause Society Recommendations for ... today in the Society,s journal Menopause . ... recommendations for the care of midlife women freely ...
Breaking Biology News(10 mins):Being social: Learning from the behavior of birds 2Phthalates heighten risk for childhood asthma 2Phthalates heighten risk for childhood asthma 3
... A team of scientists from the University Hospitals of ... bowel diseases have low bone mass and poor bone architecture ... at increased risk of fracture. Inflammatory bowel disease ... inflammation of the bowel. Two of the most common disorders ...
... new study presented today at the World Congress on ... a commonly used injectible contraceptive, is associated with higher ... in combination with estrogens. Depot medroxyprogesterone acetate ... birth control drug which is injected every 3 months. ...
... muscle tissue experiences trauma or disease, such as ... as "satellite cells" respond to repair and regenerate ... neuromuscular diseases, such as muscular dystrophy, which affect ... researchers have used time-lapse photography to document satellite ...
Cached Biology News:Caught on tape: Muscle stem cells captured on video by MU researcher 2
Anti-ADAM-12 (Meltrin Alpha); amino terminal region; rabbit host...
Mouse monoclonal [3G8] to MDMA ( Abpromise for all tested applications)....
Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 25 tests...
Rabbit Anti-melatonin (different linkage) Polyclonal Antibody...
Biology Products: