Navigation Links
Reduce Errors and Save Time When Setting Up PCRs

Polymerase chain reaction (PCR) setup typically requires thawing of the various reagents, setting up a master mix, then distributing the mix to the individual reaction tubes.

The various handling steps
  • can cause pipetting mistakes and experimental error
  • bear the risk of contaminating the amplification reaction and
  • are time-consuming.
Facilitate PCR Setup and Obtain Reliable Results with the PCR and High Fidelity PCR Masters

The PCR Master is a 2x concentrated ready-to-use master mix that contains Taq DNA Polymerase, PCR-Grade Deoxynucleotides, and PCR Reaction Buffer with a final concentration of 1.5 mM MgCl2. This mix is designed for the routine amplification of any kind of DNA up to 3 kb.
The High Fidelity PCR Master is a 2x concentrated ready-to-use master mix that contains the Expand High Fidelity Enzyme blend, PCR-Grade Deoxynucleotides, and PCR Reaction Buffer with a final concentration of 1.5 mM MgCl2. The Expand High Fidelity Enzyme blend is a blend of Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This powerful enzyme blend is designed to amplify any kind of DNA up to 5 kb (Figure 1) with higher yield and three times higher fidelity than Taq DNA Polymerase alone.

Advantages of these Master Mixes

  • Save time: Blends are stored at 4C and can be immediately used, no freezing or thawing is necessary.
  • Improve results by reducing pipeting errors: Master mixes contain all the reagents required to perform PCR. Simply add the template DNA, primers, and the required amount of PCR-Grade Water (provided with the products) .
  • Minimize contamination risks: Only two pipetting steps are necessary; both master mixes are provided as 2x concentrated ready-to-use mixes in aliquots of 10 reactions.
  • Enjoy guaranteed performance: Both master mixes are function-tested for maximum thermal stability, processivity, and specificity.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
2. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
3. Setting up the laboratory to avoid contamination
4. Setting up a kinase profiler with IMAP
5. Setting up Successful siRNA Library Screens
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/6/2016)... ... December 06, 2016 , ... RoviSys, a leading ... in Aurora, Ohio, announced the opening of their new office building today. Located ... facility is home to 200 employees focused on providing sales, engineering, and support ...
(Date:12/5/2016)... Research and Markets has announced the ... and Companies" to their offering. ... , , This ... -omics technologies such as proteomics and metabolomics. Molecular diagnostics technologies are ... based on biomarker. Currently the most important applications of ...
(Date:12/5/2016)... , December 5, 2016 ... Expansion Market, by Products (Consumable, Instruments, Automated Cell ... Medicine and Stem Cell Research, Cancer, and Cell-Based ... Cell Banks) - Global Revenue, Trends, Growth, Share, ... Market Research, the global cell expansion market is ...
(Date:12/5/2016)... , Dec. 5, 2016 NxGen MDx announced today that ... the test in house, we,ve been able to improve customer service through ... patients," says Alan Mack , CEO of NxGen MDx. ... , , A ... volume has led to more job opportunities at the Grand Rapid headquarters. ...
Breaking Biology Technology:
(Date:11/29/2016)... Nov. 29, 2016   Neurotechnology , ... object recognition technologies, today released FingerCell 3.0, ... recognition solutions that run on low-power, low-memory ... using less than 128KB of memory, enabling ... that have limited on-board resources, such as: ...
(Date:11/22/2016)... According to the new market research report "Biometric System Market by Authentication ... (Hardware and Software), Function (Contact and Non-contact), Application, and Region - Global ... from USD 10.74 Billion in 2015 to reach USD 32.73 Billion by ... Continue Reading ... ...
(Date:11/17/2016)... Global Market Watch: Primarily supported by ownership types; ... Academics) market is to witness a value of US$37.1 billion ... Compounded Annual Growth Rate (CAGR) of 10.75% is foreseen from ... 2014-2020. North America is not way ... Europe at 9.56% respectively. Report Focus: ...
Breaking Biology News(10 mins):