Navigation Links
Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

ers, one tenth of the RT reaction, and SuperTaq Polymerase were used for PCR. Each bar represents the mean standard deviation for 8 replicates. TBP=TATA Binding Protein; GAPDH=Glyceraldehyde-3-phosphate Dehydrogenase; UBC=Ubiquitin C; PKC=Protein Kinase C; Recc1=Replication Factor C; RNAPol II=RNA Polymerase II; FAS=Fatty Acid Synthase; DDPK=DNA Activated Protein Kinase.


DNA Isolation from FFPE Tissues

Researchers can now recover DNA from FFPE samples just as easily as RNA by simply increasing the digestion time to 2 days. Isolation of DNA includes an RNase treatment to fully eliminate RNA. Figure 2 shows an Agilent 2100 bioanalyzer scan of total nucleic acids, RNA, and DNA from an FFPE sample processed with the RecoverAll Kit.

Figure 2. Agilent 2100 bioanalyzer Scan Demonstrating Presence of RNA and DNA from FFPE Mouse Liver. Nucleic acids were isolated from FFPE mouse liver (20 m section, fixed and embedded using standard hospital protocol) using the RecoverAll Total Nucleic Acid Isolation Kit. Three equal amounts of sample (based on A260) were treated as follows: untreated control received no DNase or RNase treatment; RNA was isolated by DNase treatment of the nucleic acid sample; DNA was isolated by RNase treatment of the nucleic acid sample. An equal volume of each was analyzed on the Agilent 2100 bioanalyzer.


Get More Data from Your FFPE Samples

Many researchers considered their archives of FFPE tissues lost to molecular analysis. Now, with Ambion's RecoverAll Kit, you can isolate the full range of nucleic
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Recovery of DNA from LMP Agarose1
2. Efficient Recovery of Ultrapure Plasmid DNA
3. Improve Recovery and Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWXL Size Exclusion Columns
4. Yeast Protein Production System Features High Yields and One-Step Purification
5. ArrayScript RT: Maximize Yields of Full-length cDNA
6. Synthesize High Yields of Biotinylated aRNA
7. Translate High Yields of Active Protein
8. Translate High Yields of Biologically Active Proteins
9. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
10. High-Throughput Isolation of Total RNA
11. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
Post Your Comments:
(Date:1/15/2014)... DTS Language Services, Inc . is pleased to ... Science organizations who need document translations. Clients will now ... documents in advance with a selection of nearly 50 life ... a critical factor in clinical and scientific fields, and decrease ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. ... Road, Warrington, Pa. , Details: The Hepatitis B ... a cure for hepatitis B and improving the quality of ... Ball on Friday, April 11 at Warrington Country Club in ...
(Date:1/14/2014)... EquitiesIQ, a leading informational research provider, ... is an emerging biomedical company acquiring, developing, manufacturing, and ... , Free report download: http://equitiesiq.com/reports/alliqua/ , In ... team and Board, which launched the company’s new strategy ...
(Date:1/14/2014)... 2014  RXi Pharmaceuticals Corporation (OTCQX: RXII), a biotechnology ... therapies addressing major unmet medical needs using RNA-targeted ... of Allowance from the United States Patent and ... (sd-rxRNA®), for the treatment of fibrosis. The patent ...
Breaking Biology Technology:DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3
... ITGI Medical, Ltd.,(TASE: ITGI) a company engaged ... covered stents, announced that the US Food,and Drug ... to Over and Under(R) and Aneugraft(R) Pericardium Covered ... coronary arteries and,Saphenous Vein Grafts. , ...
... Md., April 28 PharmAthene, Inc. (NYSE Amex: ... against biological and chemical threats, announced today that results ... presented yesterday at the 12th Annual Conference on Vaccine ... The conference is sponsored by the National Foundation of ...
... Yesterday Bavarian Nordic Announced its Interim Results for ... in the Biodefence and Cancer Business Areas , ... of,IMVAMUNE(R) , - Preparations for the Delivery of ... - PROSTVAC(TM) Data Have Been Accepted for an Oral Presentation at ...
Cached Biology Technology:PharmAthene Presents Data for SparVax(TM), a Novel Vaccine for the Prevention and Treatment of Anthrax Infection, at the 12th Annual Conference on Vaccine Research 2PharmAthene Presents Data for SparVax(TM), a Novel Vaccine for the Prevention and Treatment of Anthrax Infection, at the 12th Annual Conference on Vaccine Research 3PharmAthene Presents Data for SparVax(TM), a Novel Vaccine for the Prevention and Treatment of Anthrax Infection, at the 12th Annual Conference on Vaccine Research 4PharmAthene Presents Data for SparVax(TM), a Novel Vaccine for the Prevention and Treatment of Anthrax Infection, at the 12th Annual Conference on Vaccine Research 5PharmAthene Presents Data for SparVax(TM), a Novel Vaccine for the Prevention and Treatment of Anthrax Infection, at the 12th Annual Conference on Vaccine Research 6PharmAthene Presents Data for SparVax(TM), a Novel Vaccine for the Prevention and Treatment of Anthrax Infection, at the 12th Annual Conference on Vaccine Research 7Bavarian Nordic Announces its Interim Report for the Period 1 January to 31 March 2009 2Bavarian Nordic Announces its Interim Report for the Period 1 January to 31 March 2009 3Bavarian Nordic Announces its Interim Report for the Period 1 January to 31 March 2009 4
(Date:4/23/2014)... and German . ... mammals, the difference between sexes depends on one single element of ... where the two sexual chromosomes are X and Y, whereas women ... all the morphological and physiological differences between males and females. , ... time ago, the X and Y were identical, until the Y ...
(Date:4/23/2014)... Wash. -- Like a hungry diner ripping open a ... into electricity must tear open a hydrogen molecule. Now ... holding onto the two halves of its hydrogen feast. ... how to make the catalyst work better for alternative ... scientists have shown precisely where the hydrogen halves end ...
(Date:4/23/2014)... and Spanish . ... released its Conservation Assessment for the Big Bend-Ro Bravo ... 29 priority conservation areas in a region straddling the ... in the states of Texas, Coahuila, and Chihuahua. This ... inhabited by rare and endangered plants and animals, and ...
Breaking Biology News(10 mins):Male or female? 2Male or female? 3Halving hydrogen 2Halving hydrogen 3Halving hydrogen 4Conservation priorities released for several protected areas along US-Mexico border 2Conservation priorities released for several protected areas along US-Mexico border 3
... Athene Donald has won the Science and Technology award ... honor to Professor Donald, Glamour magazine praised her as a ... real path for herself in the male-dominated world of physics." ... Physics at the University of Cambridge in the United Kingdom ...
... CAA researcher at Charles Drew University of Medicine and Science ... a promising, natural therapy for cancer. Dr. Mamdooh Ghoneum ... on "Cell Death Mechanism," sponsored by the American Association for ... San Diego. "The central focus of the meeting ...
... RIVERSIDE, Calif. Stem cell research at the University of ... establishment of a new Stem Cell Core Facility (SCCF) ... doing stem cell research that ordinarily would not be available ... Noel Keen Hall, had its grand opening on Friday, Jan. ...
Cached Biology News:Charles Drew cancer studies with yeast yield excellent results 2UC Riverside's new state-of-the-art technology to accelerate stem cell research 2
A component of the Acridinium protein labeling kit....
... Acridinium NHS ester can ... and nucleic acids. The covalently ... produce chemiluminescence in the presence ... ester-labeled proteins can be used ...
... again Guava is making flow cytometry accessible to ... will appreciate the latest addition to Guavas on-demand ... Mini easy to use, surprisingly affordable, very ... and 3-color detection. The Guava EasyCyte Mini has ...
... The MiniOpticon 2-color real-time PCR detection system holds 48 ... array of 48 LEDs independently excites fluorescence (470-500 nm) ... detects emitted light (523-543 nm and 540-700 nm). This ... MJ Mini thermal cycler. System dimensions are 18 x ...
Biology Products: