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Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

ers, one tenth of the RT reaction, and SuperTaq Polymerase were used for PCR. Each bar represents the mean standard deviation for 8 replicates. TBP=TATA Binding Protein; GAPDH=Glyceraldehyde-3-phosphate Dehydrogenase; UBC=Ubiquitin C; PKC=Protein Kinase C; Recc1=Replication Factor C; RNAPol II=RNA Polymerase II; FAS=Fatty Acid Synthase; DDPK=DNA Activated Protein Kinase.


DNA Isolation from FFPE Tissues

Researchers can now recover DNA from FFPE samples just as easily as RNA by simply increasing the digestion time to 2 days. Isolation of DNA includes an RNase treatment to fully eliminate RNA. Figure 2 shows an Agilent 2100 bioanalyzer scan of total nucleic acids, RNA, and DNA from an FFPE sample processed with the RecoverAll Kit.

Figure 2. Agilent 2100 bioanalyzer Scan Demonstrating Presence of RNA and DNA from FFPE Mouse Liver. Nucleic acids were isolated from FFPE mouse liver (20 m section, fixed and embedded using standard hospital protocol) using the RecoverAll Total Nucleic Acid Isolation Kit. Three equal amounts of sample (based on A260) were treated as follows: untreated control received no DNase or RNase treatment; RNA was isolated by DNase treatment of the nucleic acid sample; DNA was isolated by RNase treatment of the nucleic acid sample. An equal volume of each was analyzed on the Agilent 2100 bioanalyzer.


Get More Data from Your FFPE Samples

Many researchers considered their archives of FFPE tissues lost to molecular analysis. Now, with Ambion's RecoverAll Kit, you can isolate the full range of nucleic
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