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Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

RecoverAll Total Nucleic Acid Isolation Kit
For decades, FFPE tissue has been the staple of histologists and pathologists for creating tissue slides and making patient diagnoses. More than 400 million FFPE tissue samples exist for cancer studies alone, and many of these samples can be tied to clinical data. Using one simple kit, the RecoverAll Total Nucleic Acid Isolation Kit (patent pending), researchers can now isolate total nucleic acids (DNA and RNA, including miRNA) from the same FFPE sample. Efficient protease digestion conditions allow RNA isolation in less than one day. The nucleic acids recovered are ideal for various downstream applications such as microarray analysis, qRT-PCR, and mutation screening.


Nucleic Acid Isolation from FFPE Tissue

Ambion has developed a new method for isolating nucleic acids from human and mouse FFPE tissue. Up to four 20 m sections or unsectioned core samples <35 mg can be processed per reaction. Following deparaffinization and a rigorous protease digestion, the sample can be split into separate aliquots for isolation of total RNA (including miRNA) and genomic DNA using an optimized glass-fiber filter purification protocol.


Total RNA Isolation from FFPE Samples

Although the RecoverAll Kit cannot reverse RNA fragmentation that may have already occurred
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