Navigation Links
Recombinant HIV Reverse Transcriptase

  • More thermostable than AMV RT and M-MLV RT
  • Excellent target to evaluate antiviral agents
  • Highly sensitive enzyme for RT-PCR applications
  • Ideal for reverse transcribing transcripts with high G/C content

Like M-MLV reverse transcriptase (RT) and AMV RT, Ambion's HIV RT synthesizes cDNA from single-stranded RNA template in the presence of primer, Mg2+, and deoxyribonucleotides. HIV-RT is a recombinant enzyme purified to homogeneity from E. coli. HIV RT is a heterodimeric protein which is comprised of a 66 kDa and a 51 kDa subunit.

HIV RT is More Thermostable than M-MLV RT and AMV RT
HIV RT performs as well as M-MLV RT and AMV RT at 37C and 42C. However, the enzyme retains a significantly higher fraction of its activity at 50C than the other RTs. As shown in Figure 1, HIV RT converts a much larger proportion of a synthetic RNA transcript to cDNA than M-MLV RT and AMV RT even after the reaction has progressed for 1 hour. Thus, when working with RNA transcripts that are refractory to efficient cDNA synthesis or when the transcript being studied has a high GC content, HIV RT is a superior alternative to M-MLV RT and AMV RT.

Ambion's HIV RT is a high quality enzyme that has been tested for contaminating endonuclease and exonuclease activity. The enzyme is available in a convenient 500 U size. A 10X Reaction Buffer is suppli ed with the enzyme.

Figure 1. HIV RT Outperforms MMLV and AMV RT Enzymes in cDNA Synthesis at 50C. A synthetic RNA transcript for Xef-1 (1 g) was reverse transcribed with oligo(dT) primers in a 20 l reaction using 5 g/l M-MLV RT (Ambion), 0.5 U/l AMV RT (Seikagaku), or 0.5 g/l HIV RT (Ambion) for the indicated times. Reactions were performed using the manufacturer-supplied reaction buffer in the presence of *32-P dUTP and 0.5 U/l ribonuclease inhibitor. The percentage cDNA conversion was determined by measuring the amount of cDNA made via scintillation counting.

back to top



Page: All 1 2

Related biology technology :

1. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
2. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
3. Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column
4. Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C
5. Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)
6. Properties of Reverse Transcriptases
7. Reverse transcriptases suitable for one-step RT-PCRb
8. Reverse transcriptases suitable for two-step RT-PCRd
9. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
10. Transcriptor Reverse Transcriptase
11. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
Post Your Comments:

(Date:6/24/2016)... DIEGO , June 24, 2016 ... more sensitively detects cancers susceptible to PARP inhibitors ... circulating tumor cells (CTCs). The new test has ... HRD-targeted therapeutics in multiple cancer types. ... targeting DNA damage response pathways, including PARP, ATM, ...
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
(Date:6/23/2016)... MONICA, Calif. , June 23, 2016  The Prostate Cancer Foundation ... pioneer increasingly precise treatments and faster cures for prostate cancer. Members of the ... institutions across 15 countries. Read More About the Class ... ... ...
(Date:6/23/2016)... ... June 23, 2016 , ... In a new case report published today in ... patient who developed lymphedema after being treated for breast cancer benefitted from an injection ... for dealing with this debilitating, frequent side effect of cancer treatment. , ...
Breaking Biology Technology:
(Date:6/21/2016)... Columbia , June 21, 2016 ... to the new role of principal product architect ... named the director of customer development. Both will ... chief technical officer. The moves reflect NuData,s strategic ... in response to high customer demand and customer ...
(Date:6/16/2016)... SAN FRANCISCO , June 16, 2016 /PRNewswire/ ... Market size is expected to reach USD ... report by Grand View Research, Inc. Technological proliferation ... and banking applications are expected to drive the ... ) , The development of ...
(Date:6/9/2016)... attendance control systems is proud to announce the introduction of fingerprint attendance control software, ... employees are actually signing in, and to even control the opening of doors. ... ... ... Photo - ...
Breaking Biology News(10 mins):