Navigation Links
Recombinant HIV Reverse Transcriptase


  • More thermostable than AMV RT and M-MLV RT
  • Excellent target to evaluate antiviral agents
  • Highly sensitive enzyme for RT-PCR applications
  • Ideal for reverse transcribing transcripts with high G/C content

Like M-MLV reverse transcriptase (RT) and AMV RT, Ambion's HIV RT synthesizes cDNA from single-stranded RNA template in the presence of primer, Mg2+, and deoxyribonucleotides. HIV-RT is a recombinant enzyme purified to homogeneity from E. coli. HIV RT is a heterodimeric protein which is comprised of a 66 kDa and a 51 kDa subunit.


HIV RT is More Thermostable than M-MLV RT and AMV RT
HIV RT performs as well as M-MLV RT and AMV RT at 37C and 42C. However, the enzyme retains a significantly higher fraction of its activity at 50C than the other RTs. As shown in Figure 1, HIV RT converts a much larger proportion of a synthetic RNA transcript to cDNA than M-MLV RT and AMV RT even after the reaction has progressed for 1 hour. Thus, when working with RNA transcripts that are refractory to efficient cDNA synthesis or when the transcript being studied has a high GC content, HIV RT is a superior alternative to M-MLV RT and AMV RT.

Ambion's HIV RT is a high quality enzyme that has been tested for contaminating endonuclease and exonuclease activity. The enzyme is available in a convenient 500 U size. A 10X Reaction Buffer is suppli ed with the enzyme.

Figure 1. HIV RT Outperforms MMLV and AMV RT Enzymes in cDNA Synthesis at 50C. A synthetic RNA transcript for Xef-1 (1 g) was reverse transcribed with oligo(dT) primers in a 20 l reaction using 5 g/l M-MLV RT (Ambion), 0.5 U/l AMV RT (Seikagaku), or 0.5 g/l HIV RT (Ambion) for the indicated times. Reactions were performed using the manufacturer-supplied reaction buffer in the presence of *32-P dUTP and 0.5 U/l ribonuclease inhibitor. The percentage cDNA conversion was determined by measuring the amount of cDNA made via scintillation counting.


back to top


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
2. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
3. Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column
4. Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C
5. Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)
6. Properties of Reverse Transcriptases
7. Reverse transcriptases suitable for one-step RT-PCRb
8. Reverse transcriptases suitable for two-step RT-PCRd
9. C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR
10. Transcriptor Reverse Transcriptase
11. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/19/2017)... ... 19, 2017 , ... Molecular Devices, LLC, a leader in ... launch the CloneSelect™ Single-Cell Printer™ in North America. This novel system utilizes ... visual documentation of monoclonality for use in cell line development. , Clonal ...
(Date:9/18/2017)... ... September 18, 2017 , ... Transportable biomass conversion facilities ... torrefied wood is the topic of a September 27 webinar hosted ... viability of transportable biomass conversion facilities for producing biochar, briquettes, and torrefied wood, ...
(Date:9/18/2017)... ... September 18, 2017 , ... ... optimization firm for the life sciences and healthcare industries, announces Bryan Coddington ... , What: Digital Transformation in Medical Device – The Journey to FDA Compliant ...
(Date:9/14/2017)... ... September 14, 2017 , ... ... formally introduces its flexible scientist program (FSP)-- a flexible business approach similar ... FSP, which combines SSCI’s extensive project-based analytical and solid-state chemistry services and ...
Breaking Biology Technology:
(Date:3/27/2017)... , March 27, 2017  Catholic Health ... and Management Systems Society (HIMSS) Analytics for achieving ... Adoption Model sm . In addition, CHS previously ... U.S. hospitals using an electronic medical record (EMR). ... its high level of EMR usage in an ...
(Date:3/24/2017)... , Mar 24, 2017 Research and Markets ... System Market Analysis & Trends - Industry Forecast to 2025" ... ... grow at a CAGR of around 15.1% over the next decade ... industry report analyzes the market estimates and forecasts for all the ...
(Date:3/23/2017)... Research and Markets has announced the addition ... - Industry Forecast to 2025" report to their offering. ... The Global Vehicle ... around 8.8% over the next decade to reach approximately $14.21 billion ... estimates and forecasts for all the given segments on global as ...
Breaking Biology News(10 mins):