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Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers

uHTS: a prototypic 1536-well fluorescence anisotropy-based uHTS screen and application of well-level quality control procedures. J. Biomol. Screen. 6:275-290.

8. Sportsman, J.R., Lee S.K., Dilley, H., et al.: Fluorescence Polarization. In Devlin, J.P. (ed): High Throughput Screening: The Discovery of Bioactive Substances. New York: Marcel Dekker, 1997:389-399.

9. Banks, P., Gosselin, M., Prystay, L. (2000). Impact of a red-shifted dye label for high throughput fluorescence polarization assays of G protein-coupled receptors. J. Biomol. Screen. 5:329-334.

10. Prystay, L., Gosselin, M., Banks, P. (2001). Determination of equilibrium dissociation constants in fluorescence polarization. J. Biomol. Screen. 6:141-150.

11. Owicki J.C. (2000) Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer. J. Biomol. Screen . 5:297-306.

12. Zhang, J.-H., Chung, T.D.Y., Oldenburg, K.R. (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4:67-73.


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