Navigation Links
Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye

0.993 was obtained. Two samples of human genomic DNA were also tested. By interpolation of the standard curve, the first patient sample was determined to have an initial concentration of 254 copies/l, the second had 357 copies/l.

Specificity of the PCR was measured in two ways. First, melting curve analysis was performed on each sample. For example, the melting curve analysis for patient sample 1 revealed a single peak at 88.5C, which indicates exclusive amplification of the -actin amplicon as seen in Figure 4. Second, gel electrophoresis was performed to analyze the PCR products. Figure 5 reveals single bands for each standard and sample, indicating specific amplification of the desired product with only minor amplification of a primer-dimer.


Discussion
Broad Dynamic Range for Lambda and Human DNA
DyNAzyme II DNA polymerase can be used successfully with SYBR Green I dye to quantify DNA from small genomes as well as the more complex human genome. Experiments revealed a linear relationship between log starting copy number and C(t) value over at least 6 orders of magnitude for the lambda genomic DNA template, and over at least 3 orders of magnitude for the human genomic DNA template. The broad dynamic range of starting template that can be detected makes this assay particularly valuable when sample DNA concentrations are unknown or vary widely.

In this study, the lower limit of the standard range was 100 copies of lambda DNA, or 75 copies of human genomic DNA per reaction. Other studies have shown linear results below this level. Though not necessary in this application, detection down to several copies of template could b
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
2. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
3. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
6. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
7. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
8. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
9. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
10. Real-Time PCR: General Considerations, Rev A
11. Quantification made easy
Post Your Comments:
(Date:4/24/2015)... Texas (PRWEB) April 24, 2015 ... for use in applications such as animal waste ... and oil reduction in wastewater treatment plants and ... an exhibitor at the National Restaurant Association’s NRA ... , Representatives of the company will be ...
(Date:4/24/2015)... 2015 PAREXEL International Corporation (NASDAQ: ... announced that Partnerships in Clinical Trials named PAREXEL,s ... Clinical Innovator of the Year.  The honor recognizes Mr. ... Company,s Perceptive MyTrials ® Data-Driven Monitoring solution. ... the 2015 Partnership Awards in Boston ...
(Date:4/23/2015)... 23, 2015 /CNW/ - A new and innovative service ... North America,s largest packaging ... Silver in the "Sustainability, service" category of the ... "Competitor Comparison" is a service that scores clients, ... Global peers. This benchmarking process shows clients how ...
(Date:4/23/2015)... April 23, 2015 Follow ... to a series of genetic tests that determine ... Lou Gehrig's disease, Huntington's disease, and Alzheimer's disease. ... gene composition and interaction in diseased tissue/cells to ... well as the response to treatment, such as ...
Breaking Biology Technology:DryLet to Showcase Bio-React at the National Restaurant Association’s NRA Show 2015 May 16-19, 2015 in Chicago, Illinois 2PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 2PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 3PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 4PAREXEL Honored With 2015 Clinical Innovator Of The Year Award From Partnerships In Clinical Trials 5Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 2Increasing Disease Prevalence & Focus on Preventive Healthcare Drive the Predictive Diagnostics Market, According to a New Report by Global Industry Analysts, Inc. 3
... , ... • LEADseeker • DeepBlueC• cell-based assays , ... Competitive pressure is forcing organizations to continually increase screening ... screens such as luminescence cell-based assays. LEADseeker™ Multimodality Imaging System was ...
... , Key ... amplification (WGA),• degenerate oligonucleotideprimed PCR (DOP-PCR),• Cy3/Cy5-dCTP probe labeling ... array • array CGH ... Human genomic DNA isolated from formalin-fixed ...
... Amounts of Data into Gems of Knowledge Using discoveryHub™ ... Abstract , This paper discusses key problems and ... (volume) and complexity of life science data available today, ... integration that enables rapid ‘harvesting’ and analysis of critical ...
Cached Biology Technology:Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System 2Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System 3GenomiPhi amplification of FFPE tissue DNA for array CGH 2GenomiPhi amplification of FFPE tissue DNA for array CGH 3GenomiPhi amplification of FFPE tissue DNA for array CGH 4GenomiPhi amplification of FFPE tissue DNA for array CGH 5GenomiPhi amplification of FFPE tissue DNA for array CGH 6GenomiPhi amplification of FFPE tissue DNA for array CGH 7GenomiPhi amplification of FFPE tissue DNA for array CGH 8GenomiPhi amplification of FFPE tissue DNA for array CGH 9GenomiPhi amplification of FFPE tissue DNA for array CGH 10Exploiting the Life Science Data Explosion to Speed New Drug Discovery 2Exploiting the Life Science Data Explosion to Speed New Drug Discovery 3Exploiting the Life Science Data Explosion to Speed New Drug Discovery 4Exploiting the Life Science Data Explosion to Speed New Drug Discovery 5Exploiting the Life Science Data Explosion to Speed New Drug Discovery 6Exploiting the Life Science Data Explosion to Speed New Drug Discovery 7Exploiting the Life Science Data Explosion to Speed New Drug Discovery 8Exploiting the Life Science Data Explosion to Speed New Drug Discovery 9Exploiting the Life Science Data Explosion to Speed New Drug Discovery 10Exploiting the Life Science Data Explosion to Speed New Drug Discovery 11Exploiting the Life Science Data Explosion to Speed New Drug Discovery 12Exploiting the Life Science Data Explosion to Speed New Drug Discovery 13Exploiting the Life Science Data Explosion to Speed New Drug Discovery 14Exploiting the Life Science Data Explosion to Speed New Drug Discovery 15Exploiting the Life Science Data Explosion to Speed New Drug Discovery 16Exploiting the Life Science Data Explosion to Speed New Drug Discovery 17
(Date:3/24/2015)... BEACH GARDENS, Fla. , March 24, 2015 ... biometric identity management solutions, today announced that its ... premium clothing accessory retailer Goorin Bros . ... The Crossmatch biometrics-based solution secures access to the ... from the increased risk of data breaches. ...
(Date:3/23/2015)... OXFORD, Conn. , Mar. 23, 2015 NXT-ID, Inc. ... authentication company focused on the growing mobile commerce market, announces ... a new advertising campaign on CNBC television starting March 30 ... ads will commence airing in New York ... Executive Officer said: "We are excited about our new ad ...
(Date:3/20/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/vkzvcs/iris_biometrics ) ... Global Strategic Business Report" report to their offering. ... in US$ Thousands. The report provides separate comprehensive analytics for ... , Europe , Asia-Pacific ... Latin America . Annual estimates and ...
Breaking Biology News(10 mins):Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5
... February 1, 2011. Fact: The sex of some species ... warm nests produce females, cooler nests, males. And although ... years, scientists now report that almost half of the turtle ... global warming may affect turtle reproduction. To bring attention to ...
... species present a serious environmental, economic and social problem ... biodiversity and ecosystem functions, such as nutrient cycling. Despite ... can dominate new habitats over native plants that technically ... tested assumption is that these plants are more abundant ...
... key to preventing deaths from measles. Despite the availability ... children worldwide, particularly in less-developed regions where vaccination coverage ... at the Johns Hopkins Bloomberg School of Public Health ... a dry powder, live-attenuated measles vaccine that can be ...
Cached Biology News:Turtle populations affected by climate, habitat loss and overexploitation 2Home and away: How do invasive plant species dominate native species? 2Researchers test inhalable measles vaccine 2
... Spike-In Kit contains in vitro synthesized, polyadenylated ... control probes on Agilents microarrays, with minimal ... a positive microarray control in the hybridization ... is a required component of the Agilent ...
... ProtoArray high-density functional protein microarray is the ... of proteinprotein interactions on a proteome scale. ... open reading frames (ORFs) expressed as 5'-GST ... a 1 inch x 3 inch nitrocellulose-coated ...
... of cells • Blade design minimizes cell ... growth surface • Designed for use in ... • Sterilized by gamma radiation and certified ... new product number, created to easily match ...
... Designed for use with dishes and cell ... a new product number, created to easily ... availability yet, please order under the old ... for assistance. Comp Dim: blade L 1.9 ...
Biology Products: