Navigation Links
Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye

emplate more quickly generate the amount of product necessary to be detected with SGI. The linear range extends from 108 to 102 copies of lambda DNA initially present in the reaction. A plot of log quantity vs. C(t) cycle for the 100bp amplicon is shown in Figure 1b. The regression coefficient is 0.991, indicating a strong linear correlation.

The precision of this assay was measured by calculating the variation in C(t) values across the four replicates at each template concentration. The standard deviation for C(t) values was found to be 0.3 units or less as shown in Table 3.

Figure 2 shows the melting curves for the four replicate lambda DNA standards containing 104 copies per reaction. The curve shows the typical smooth decline in fluorescence with an increase in temperature as the strands of dsDNA dissociate and bound SGI is released. The negative first derivative calculation is also shown. Here, the point of inflection for the curve, the melting point (Tm), is clearly seen as a single, common peak at approximately 82C. This peak corresponds to the predicted melting temperature of the amplicon, indicating amplification of the amplicon of interest. Anomalies due to contamination, primer-dimer, false priming, etc., are not evident in the negative first derivative plot, as indicated by the lack of additional peaks or of abnormal broadening of the single peak.

Human Genomic DNA Template: Patient Samples Quantified Using a Standard Curve
A standard curve generated from human genomic DNA is shown in Figure 3. A linear curve in the range of 7.5x104 copies/reaction to 75 copies/reaction with r2=
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
2. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
3. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
6. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
7. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
8. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
9. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
10. Real-Time PCR: General Considerations, Rev A
11. Quantification made easy
Post Your Comments:
(Date:1/22/2015)... January 22, 2015 Dr. Greg Leyer of ... annual Scripps Natural Supplements Pre-Conference seminar on probiotics in San ... an annual continuing education conference for health care professionals. This ... the topic of probiotics in health. Dr. Leyer spoke about ...
(Date:1/22/2015)... 22, 2015 Selexis SA, a serial ... Cell Banks (RCBs) used for drug discovery to commercial ... will include Next-Generation Sequencing (NGS) data packages. ... biologic manufacturing by ensuring the integrity of the gene, ...
(Date:1/22/2015)... , 22 de enero de 2015  El Dr. ... su llamada a nominaciones para 2015. Este prestigioso premio ... o tiene el potencial para hacer, contribuciones destacadas para ... aceptarán hasta el 15 de marzo de 2015 a ...
(Date:1/22/2015)...  Varian Medical Systems (NYSE: VAR ), world leader ... commitment to sustainability with inclusion on a prestigious list of ... healthcare equipment company among the Corporate Knights Global 100 Most ... at Davos, Switzerland . ...
Breaking Biology Technology:Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Varian Honored Among World's 100 Most Sustainable Corporations 2
... Larissa Tan, Bio-Rad Laboratories, Inc.,Hercules CA 94547 USA , ... General Information , The ... in molecular biology. The use of this technique has generated unprecedented ... expression analysis. , In addition, new techniques that ...
... , Larissa Tan, Marni Brisson, Robert Park, and Keith ... Hercules, CA 94547 , USA , ... , The detection of fluorescence resonance ... reaction (PCR) and melt-curve analysis is a , ...
... Bowes Rickman 1, 2, 1 Departments of , ... NC 27710 USA , Correspondence: Catherine ... Medical Center, Box 3802, Durham, NC 27710, Phone (919) , ... Introduction , ...
Cached Biology Technology:Real-Time PCR: General Considerations, Rev A 2Real-Time PCR: General Considerations, Rev A 3Real-Time PCR: General Considerations, Rev A 4Real-Time PCR: General Considerations, Rev A 5Real-Time PCR: General Considerations, Rev A 6Real-Time PCR: General Considerations, Rev A 7Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 2Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 3Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 4Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 5Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 6Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A 7Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 2Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 3Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 4Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 5Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 6Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 7Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A 8
(Date:1/22/2015)... 15, 2015  BellBrook Labs, a leader in high ... launch of a TR-FRET (time resolved Forster resonance ... Assay, a high throughput screening assay for glycosyltransferases ... allow for sensitive detection of hundreds of human ...
(Date:1/22/2015)... MUNICH , January 22, 2015 ... its tenth year  The European Patent Office to present ... Two British nominations to be featured: Christofer Toumazou ... week featuring former finalists and winners of the Award   ...
(Date:12/22/2014)... , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: NXTD ... company focused on the growing mobile commerce market, announces it ... Robbins for the 2015 International CES debut of the ... will be at the NXT-ID booth January 6th and ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4
... a pointer point, a sheep dog herd, and a retriever ... Danes? And how can a tiny Chihuahua possibly be ... in size, shape, color, coat length and behavior more than ... been unexplained. Now, scientists have developed a method to identify ...
... Smithsonian,s National Zoo detected a secondary rise in urinary ... Xiang (may-SHONG) earlier this month. The results from the ... the hormone rise indicates that it would be mid- ... a cub or comes to the end of a ...
... ARGONNE, Ill. (June 20, 2008) The future of ... scientists at U.S. Department of Energy,s Argonne National Laboratory ... sources. "The free electron laser oscillator (X-FELO) we ... million times brighter than currently operating machines," Argonne Distinguished ...
Cached Biology News:DNA study unlocks mystery to diverse traits in dogs 2A novel X-ray source could be brightest in the world 2
... The Schleicher & Schuell BioScience ... center dedicated to custom protein and ... experienced project teams, comprised of R&D, ... work closely with collaborators every step ...
... acid synthetic peptide whose sequences are derived from mouse resistin protein. ... S(21) - S - M - P - L - C ... I - D - K - K - I - K ... be used for neutralization and control experiments with the polyclonal antibody ...
... Molecular Imaging (MI) Software for use in ... and drug discovery applications. The Regulatory Edition ... Drug Administrations Code of Federal Regulations Title ... [21 CFR Part 11]. KODAK MI Software, ...
... for pathway studies, protein-protein interaction studies, ... built on the flexible and powerful ... microarrays are available as part of ... Custom Probe Content ...
Biology Products: