Navigation Links
Real-Time PCR: General Considerations, Rev A

Faye Boeckman, Marni Brisson, and Larissa Tan, Bio-Rad Laboratories, Inc., Hercules CA 94547 USA


General Information
The polymerase chain reaction (PCR) has proven to be a versatile tool in molecular biology. The use of this technique has generated unprecedented advances in gene discovery, diagnostics, and gene expression analysis. In addition, new techniques that build on PCR have further expanded its range of scientific applications.

Real-time PCR is a powerful advancement of the basic PCR technique. Through the use of appropriate fluorescent detection strategies in conjunction with proper instrumentation, the starting amount of nucleic acid in the reaction can be quantitated. Quantitation is achieved by measuring the increase in fluorescence during the exponential phase of PCR. Applications of real-time PCR include measurements of viral load, gene expression studies, clinical diagnostics, and pathogen detection.

Although the performance of PCR in more routine molecular biology applications can be relatively straightforward to optimize, several parameters must be evaluated and optimized independently to achieve the maximum potential of real-time PCR. The factors that affect real-time PCR fall into 3 categories. These are general laboratory practices, template and primer design, and reaction components and conditions. When determining which conditions to optimize, the ultimate assay goal (i.e., qualitative analysis vs. quantitation) must be considered.

To develop sensitive real-time PCR assays cost effectively, you should design and optimize the primer sets prior to developing the probe. This technical note will guide you in the development of an opti
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
2. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
3. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
4. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
5. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
6. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
7. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
8. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
9. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
10. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
11. General Notes on Primer Design in PCR*
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Real Time PCR General Considerations Rev

(Date:7/22/2014)... July 22, 2014 Nuclear reactors are ... produced by controlled fission chain reaction in nuclear reactors ... is used to drive a power- generating turbine. Currently ... the world’s total electricity production. Nuclear reactor market is ... construction, planned and proposed nuclear reactor projects. , Early ...
(Date:7/22/2014)... Rice Lake, Wisconsin (PRWEB) July 22, 2014 ... will be in attendance at the 2014 NCSL ... Florida. Booth #423 will feature CONDEC pressure ... balance style weights, and the new, customized sets, ... years ago to promote cooperative efforts in solving ...
(Date:7/22/2014)... Parsippany, NJ (PRWEB) July 22, 2014 ... analytical, precision, portable, and mechanical balances and scales, ... precision balances has won Laboratory Equipment magazine’s 2014 ... is the second consecutive year the Explorer was ... readers. The annual Readers’ Choice Awards celebrates “excellence ...
(Date:7/22/2014)... SHENZHEN , Cina, 22 luglio 2014 ... una filiale di BGI, la maggiore organizzazione di genomica ... nuovo servizio di sequenziamento dell,intero esoma umano con base ... Complete Genomics, molto apprezzata nel settore per ... con il 99.999% di precisione e una rilevazione altamente ...
Breaking Biology Technology:Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3Rice Lake Weighing Systems Showcases Metrology Solutions for the 2014 NCSL International Workshop & Symposium 2OHAUS Explorer Voted “Best Balance” in Laboratory Equipment’s 2014 Readers’ Choice Awards 2BGI Tech lancia un servizio di sequenziamento dell'intero esoma umano sulla piattaforma avanzata di Complete Genomics 2
... 10 Endologix, Inc. (Nasdaq: ELGX ), developer of ... Lemaitre, former President and CEO of CoreValve, has joined the ... now has four independent directors and three non-independent directors. , ... pleased to welcome Dan Lemaitre to the Endologix Board of ...
... , ... signs of growth, Sidoti & Company, Wall Street,s largest, fastest-growing small cap ... unique investment opportunities within the micro cap market at the firm,s ... Grand Hyatt Hotel in New York, NY. , "Micro cap ...
... 10 /PRNewswire-FirstCall/ - YM BioSciences Inc. (NYSE Amex: YMI ... identifies and advances a diverse portfolio of promising cancer-related products ... the proposed transaction which would result in the merger of ... October 5, 2009. The board of directors of Cytopia has ...
Cached Biology Technology:Endologix Appoints Dan Lemaitre to its Board of Directors 2Technology, Biotech, Energy and Transportation Among the Industries to be Featured at Sidoti & Company's First Annual 2010 Micro Cap Conference 2YM BIOSCIENCES OFFER FOR CYTOPIA APPROVED TO PROCEED TO SHAREHOLDER VOTE BY AUSTRALIAN COURT 2YM BIOSCIENCES OFFER FOR CYTOPIA APPROVED TO PROCEED TO SHAREHOLDER VOTE BY AUSTRALIAN COURT 3YM BIOSCIENCES OFFER FOR CYTOPIA APPROVED TO PROCEED TO SHAREHOLDER VOTE BY AUSTRALIAN COURT 4
(Date:7/22/2014)... allergic to dust mites (and chances are you are), ... the University of Iowa have developed a vaccine that ... immune response. In animal tests, the nano-sized vaccine package ... to the allergens, according to the paper, published in ... Journal . One big reason why it works, ...
(Date:7/22/2014)... An international collaboration known as the ,1000 Bull Genomes ... beef and dairy cattle while also improving animal health ... based on sequencing the whole genomes of 234 individual ... millions -- are reported in the journal Nature ... could use this information to reduce or eliminate hereditary ...
(Date:7/22/2014)... that split light with uncanny precision are key ... computers that manipulate light instead of electricity. But ... etching them with a precise beam of electrons, ... researchers at Princeton and Columbia universities have proposed ... customize and grow these specialized materials, known as ...
Breaking Biology News(10 mins):Researchers create vaccine for dust-mite allergies 2Whole-genome sequencing of bulls in key beef and dairy breeds 2Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3
... -- The Damon Runyon Cancer Research Foundation, a ... researchers and innovative cancer research, named 11 new ... Award Committee review. The recipients of this ... basic and translational cancer research in the laboratories ...
... just how big a carbon footprint is created by travel to ... kind in the world to look at this issue. It also ... as: Setting up audio/video facilities at airports ... to airports to see off friends and family., Situating luggage-drop facilities ...
... NARRAGANSETT, R.I. January 21, 2010 The bubbles ... and tickle your nose are exhibiting a behavior quite similar to ... physicist Helen Czerski. But while the champagne bubbles ... cause clouds to form and affect the climate. "Bubbles are ...
Cached Biology News:Damon Runyon Cancer Research Foundation awards prestigious fellowships to 11 top young scientists 2Damon Runyon Cancer Research Foundation awards prestigious fellowships to 11 top young scientists 3Saying goodbye at airports the green way 2Bubble physicist counts bubbles in the ocean to answer questions about climate, sound, light 2Bubble physicist counts bubbles in the ocean to answer questions about climate, sound, light 3
SHP-2 Antibody...
Anti human PPAR gamma2 mouse monoclonal antibody...
... The antiserum has been tested on tissue ... staining pattern obtained with AB1773 corresponds to ... with probes to GLYT2 mRNA and to ... Preabsorption of the antiserum with the immunogen ...
Mouse monoclonal [RIZ1723] to PRDM2/RIZ...
Biology Products: