( high- (90%) and equal-efficie...)Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

high- (>90%) and equal-efficiency PCR amplifications of both genes. Amplification efficiencies of the GAPDH and α-tubulin plasmids were determined prior to multiplexing and found to be nearly equal and nearly 100% (data not shown).

Gene expression studies of multiple gene targets, as well as other applications, require both precise and accurate quantitation of the same samples at the same time. The optimization of PCR efficiency is essential in performing this accurate quantitation. We have demonstrated that the iCycler iQ system is a powerful tool to achieve this optimization and to perform multiplex real-time PCR with plasmid and genomic targets.

Reference
Heid CA et al., Real time quantitative PCR, Genome Res 6, 986994 (1996)

Cy is a trademark of Amersham Pharmacia International PLC. Texas Red is a trademark of Molecular Probes, Inc.

* Practice of the patented polymerase chain reaction (PCR) process requires a license. The iCycler iQ system includes a licensed thermal cycler and may be used with PCR licenses available from Applera Corporation. Its use with authorized reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. Some applications may require licenses from other parties.

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