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Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells

http://www.ambion.com/techlib/tn/116/8.html">MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers) to screen miRNA expression levels in six common cell types (Figure 2) and chose three that had very low levels of miR-1b, let-7a, and miR-196. We then transfected the selected cells with the three Pre-miR miRNAs listed above and measured the expression of the target genes in miRNA- and negative control-transfected cells. Consistent with published reports, we found that cells transfected with the appropriate Pre-miR miRNAs exhibited reduced activity or expression of the target genes by as much as 80% (Figure 3).

Figure 2. Relative miRNA Expression Among Cell Types. The miRNA expression profiles of seven common cell types were assessed using the miRNA array procedure (see Novel MicroRNA Array Technology for Sensitive miRNA Profiling). miRNAs that generate very high signal on the microarray are denoted by ++++, high-signal miRNAs are denoted by +++, medium-signal miRNAs are denoted by ++, and low-signal miRNAs are denoted by +. A "0" indicates an miRNA with signal that does not exceed that of negative control elements on the microarray. Here a subset of miRNAs expressed in these cells is shown. [Full Table]

Figure 3. Reduction in Endogenous Gene Expression by Synthetic Pre-miR miRNA. HeLa, HepG2, and A549 were transfected with Pre-miR miRNAs for hsa-mir-1b (miR-1b), hsa-let-7a (let-7a), and hsa-mir-196 (miR-196), respectively, u
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