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dure that is optimized for high throughput analyses -- both for manual processing and on robotic platforms.


Compatible with TaqMan Gene Expression Assays
Applied Biosystems pre-designed primer and probe sets for real-time PCR are one of the most commonly used methods for measuring changes in gene expression by qRT-PCR. The Cells-to-Signal technology is compatible with TaqMan Gene Expression Assays (formerly known as Assays-on-Demand). Figures 1 and 2 show data generated in one-step real-time RT-PCR using TaqMan Gene Expression Assays for human GSK-3 and VEG-F. In addition, the data (Figure 2) also demonstrate that the Cells-to-Signal procedure is sensitive enough to detect target in lysate equivalent to 3 cells.

Figure 1. qRT-PCR Detection of GSK-3 Using TaqMan Gene Expression Assays and Cells-to-Signal. HeLa cells were lysed at concentrations of 1000, 200, 40, 8, and 1.6 cells/l according to the Cells-to-Signal lysis procedure. 6 l of cell lysate was used in a 20 l one-step RT-PCR using a TaqMan Gene Expression Assay primer and probe set specific for GSK-3.

Figure 2. qRT-PCR Detection of VEG-F Using TaqMan Gene Expression Assays and Cells-to-Signal. HeLa cells were lysed at concentrations of 1000, 100, 10 and 1 cells/l according to the Cells-to-Signal lysis procedure. 3 l of cell lysate was used in a 10 l one step RT-PCR using a TaqMan Gene Expression Assay primer and probe set specific for VEG-F.


Measuring siRNA Induced Knockdown
siRNA induced knockdown of gene expression is now one of the most widely used
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