Navigation Links
Rapidly Synthesize Large Amounts of Capped RNA

Convenient format - premixed cap analog and nucleotides provided

Optimized for highest yield of capped RNA

80% capping efficiency

Complete with SUPERaseIn RNase inhibitor, DNase I, and positive control

Ideal for microinjection, in vitro or in vivo translation

The mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high yield transcription technology. Each reaction synthesizes 10-50 times the yield of capped RNA obtained with conventional in vitro transcription reactions.

The reaction is simple. DNA template is mixed with the reaction components and incubated at 37C for up to 2 hours. Figure 1 shows a time course experiment that plots yield of a 3.7 kb capped transcript over time. With this template, the mMESSAGE mMACHINE reaction is complete in only 30 minutes. In addition to synthesizing high yields of capped RNA transcripts (Figure 1), mMESSAGE mMACHINE reactions also produce full-length transcripts. Figure 2 shows an Agilent bioanalyzer 2100 electropherogram of the 3.7 kb transcript. The presence of a single, strong peak indicates the predominance of full length transcript. This is also evident in Figure 3, in which mMESSAGE mMACHINE reactions producing 1.7 kb, 3.7 kb and 9 kb transcripts were analyzed on a conventional denaturing agarose gel.

Figure 1. mMESSAGE mMACHINE Reaction Time Course. A 3.7 kb template was transcribed in 3 replicate mMESSAGE mMACHINE reactions as per protocol. After the indicated incubation times, each reaction was DNase treated as per protocol and a preliminary quantitatation was performed with RiboGreen (Molecular Probes). Each reaction was then purified using the MEGAclear Kit (Ambion) and quantitated by UV spectrophotometry.

Figure 2. Electropherogram of a mMESSAGE mMACHINE Reaction. A 3.7 kb template was used in a 20 l mMESSAGE mMACHINE reaction as per protocol and incubated for 30 min followed by analysis using the Agilent 2100 bioanalyzer.

Figure 3. Gel Analysis of mMESSAGE mMACHINE Reactions. Templates generating transcripts of sizes 1.7 kb, 3.7 kb and 9 kb were used in a mMESSAGE mMACHINE reaction as per protocol. One g of each reaction was denatured using NorthernMax-Gly Sample Loading Dye and analyzed on a 1% agarose gel with EtBr.

Cap analog is premixed with GTP at a ratio optimized for the best compromise between yield and proportion of capped transcripts (80%). The cap analog:GTP ratio can be adjusted with the GTP Solution provided in the kit to increase yields of long transcripts (>4 kb); in fact, RNA transcripts as long as 23 kb have been synthesized with mMESSAGE mMACHINE. The kit includes SUPERase In RNase Inhibitor . SUPERase In inhibits RNases A, B, C, 1 and T1 and offers the greatest available protection against RNases (see data). DNase I and a positive control DNA template are also included. The RNA synthesized from a mMESSAGE mMACHINE reaction is ideal for in vitro or in vivo translation studies.

Ambion also offers the mMESSAGE mMACHINE T7 Ultra Kit for the generation of RNA transcripts capped with Anti Reverse Cap Analog (ARCA). A base modification in this cap analog generates RNAs with ARCA incorporated only in the functional, translatable orientation, resulting in higher protein yields. The mMESSAGE mMACHINE T7 Ultra Kit includes reagents for both transcription and the addition of a poly(A) tail to capped transcripts.

back to top

Ordering Information
Cat# Product Name Size 1340 mMESSAGE mMACHINE SP6 Kit 25 rxns 1344 mMESSAGE mMACHINE T7 Kit 25 rxns 1345 mMESSAGE mMACHINE T7 ULTRA Kit 10 rxns 1348 mMESSAGE mMACHINE T3 Kit 25 rxns


Page: All 1 2 3 4

Related biology technology :

1. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
2. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
3. Rapidly Prepare Probes and Markers for Small RNA Analysis
4. Synthesize High Yields of Biotinylated aRNA
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. PCR-Grade Nucleotides Larger Pack Sizes Now Available
7. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
8. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
9. Constructing Directional cDNA Libraries from Limited Amounts of RNA
10. Detecting Attomole Amounts of Small RNA
Post Your Comments:

(Date:11/30/2015)... 30, 2015 /PRNewswire/ - Zenith Epigenetics Corp. ("Zenith" or the ... C.W. Wong to its Board of Directors to replace ... with a wealth of experience as co-founder of Resverlogix, with ... --> --> Dr. Wong remarked, ... of directors. Zenith,s long standing expertise in epigenetics and the ...
(Date:11/30/2015)... -- TapImmune, Inc. (TPIV), a clinical-stage ... and gene-based immunotherapeutics and vaccines for the treatment of ... presenting at the 8 th Annual LD Micro ... PT. Dr. John N. Bonfiglio a TapImmune ... presentation and will join TapImmune management in meeting with ...
(Date:11/30/2015)... 2015  Northwest Biotherapeutics (NASDAQ: NWBO ) ("NW ... therapies for solid tumor cancers, announced today that the ... and the Company welcomes Neil Woodford,s call ... anonymous internet report on NW Bio.  The Company,s Board ... Powers stated, "We agree with Mr. Woodford that, ...
(Date:11/27/2015)... , ... November 27, 2015 , ... ... that includes over 2,000 technical presentations offered in symposia, oral sessions, workshops, ... applied spectroscopy, covers a wide range of applications such as, but not limited ...
Breaking Biology Technology:
(Date:11/4/2015)... York , November 4, 2015 ... a new market report published by Transparency Market Research "Home ... Growth, Trends and Forecast 2015 - 2022", the global home ... US$ 30.3 bn by 2022. The market is estimated ... forecast period from 2015 to 2022. Rising security needs ...
(Date:10/29/2015)... RESTON, Va. , Oct. 29, 2015 ... announced today that it has released a new version ... Daon customers in North America ... gains. IdentityX v4.0 also includes a FIDO UAF ... customers are already preparing to activate FIDO features. These ...
(Date:10/29/2015)... , Oct. 29, 2015 Today, ... a partnership with 2XU, a global leader in ... a smart hat with advanced bio-sensing technology. The ... athletes to monitor key biometrics to improve overall ... partnership, the two companies will bring together the most ...
Breaking Biology News(10 mins):