Navigation Links
Rapidly Synthesize Large Amounts of Capped RNA


Convenient format - premixed cap analog and nucleotides provided

Optimized for highest yield of capped RNA

80% capping efficiency

Complete with SUPERaseIn RNase inhibitor, DNase I, and positive control

Ideal for microinjection, in vitro or in vivo translation


The mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high yield transcription technology. Each reaction synthesizes 10-50 times the yield of capped RNA obtained with conventional in vitro transcription reactions.

The reaction is simple. DNA template is mixed with the reaction components and incubated at 37C for up to 2 hours. Figure 1 shows a time course experiment that plots yield of a 3.7 kb capped transcript over time. With this template, the mMESSAGE mMACHINE reaction is complete in only 30 minutes. In addition to synthesizing high yields of capped RNA transcripts (Figure 1), mMESSAGE mMACHINE reactions also produce full-length transcripts. Figure 2 shows an Agilent bioanalyzer 2100 electropherogram of the 3.7 kb transcript. The presence of a single, strong peak indicates the predominance of full length transcript. This is also evident in Figure 3, in which mMESSAGE mMACHINE reactions producing 1.7 kb, 3.7 kb and 9 kb transcripts were analyzed on a conventional denaturing agarose gel.

Figure 1. mMESSAGE mMACHINE Reaction Time Course. A 3.7 kb template was transcribed in 3 replicate mMESSAGE mMACHINE reactions as per protocol. After the indicated incubation times, each reaction was DNase treated as per protocol and a preliminary quantitatation was performed with RiboGreen (Molecular Probes). Each reaction was then purified using the MEGAclear Kit (Ambion) and quantitated by UV spectrophotometry.

Figure 2. Electropherogram of a mMESSAGE mMACHINE Reaction. A 3.7 kb template was used in a 20 l mMESSAGE mMACHINE reaction as per protocol and incubated for 30 min followed by analysis using the Agilent 2100 bioanalyzer.

Figure 3. Gel Analysis of mMESSAGE mMACHINE Reactions. Templates generating transcripts of sizes 1.7 kb, 3.7 kb and 9 kb were used in a mMESSAGE mMACHINE reaction as per protocol. One g of each reaction was denatured using NorthernMax-Gly Sample Loading Dye and analyzed on a 1% agarose gel with EtBr.


Cap analog is premixed with GTP at a ratio optimized for the best compromise between yield and proportion of capped transcripts (80%). The cap analog:GTP ratio can be adjusted with the GTP Solution provided in the kit to increase yields of long transcripts (>4 kb); in fact, RNA transcripts as long as 23 kb have been synthesized with mMESSAGE mMACHINE. The kit includes SUPERase In RNase Inhibitor . SUPERase In inhibits RNases A, B, C, 1 and T1 and offers the greatest available protection against RNases (see data). DNase I and a positive control DNA template are also included. The RNA synthesized from a mMESSAGE mMACHINE reaction is ideal for in vitro or in vivo translation studies.

Ambion also offers the mMESSAGE mMACHINE T7 Ultra Kit for the generation of RNA transcripts capped with Anti Reverse Cap Analog (ARCA). A base modification in this cap analog generates RNAs with ARCA incorporated only in the functional, translatable orientation, resulting in higher protein yields. The mMESSAGE mMACHINE T7 Ultra Kit includes reagents for both transcription and the addition of a poly(A) tail to capped transcripts.


back to top


Ordering Information
Cat# Product Name Size 1340 mMESSAGE mMACHINE SP6 Kit 25 rxns 1344 mMESSAGE mMACHINE T7 Kit 25 rxns 1345 mMESSAGE mMACHINE T7 ULTRA Kit 10 rxns 1348 mMESSAGE mMACHINE T3 Kit 25 rxns
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
2. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
3. Rapidly Prepare Probes and Markers for Small RNA Analysis
4. Synthesize High Yields of Biotinylated aRNA
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. PCR-Grade Nucleotides Larger Pack Sizes Now Available
7. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
8. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
9. Constructing Directional cDNA Libraries from Limited Amounts of RNA
10. Detecting Attomole Amounts of Small RNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Rapidly Synthesize Large Amounts Capped RNA

(Date:9/18/2014)... (PRWEB) September 18, 2014 Bipartisan passage ... a bill designed to stimulate development and commercialization of ... being praised by leaders of SPIE, the international ... Revitalizing American Manufacturing and Innovation (RAMI) Act, now moves ... authors have said they are optimistic that it will ...
(Date:9/18/2014)... September 18, 2014 A novel robotic ... MRI scanner is currently being tested as part of ... Hospital in Boston with the aim of determining if ... make prostate cancer biopsies faster, more accurate, less costly, ... also has the potential to deliver prostate cancer therapies ...
(Date:9/18/2014)... On Tuesday, Secretary of Energy Ernest Moniz joined ... Lab Day on the Hill. The event highlighted several ... Durbin and Risch also formally launched the Senate National ... reach of the national labs as leaders in developing ... our nation's most pressing challenges. , "The national labs ...
(Date:9/18/2014)... Research and Markets has announced the addition ... In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to ... The global companion diagnostics market is expected to grow ... Factors such as rising need for personalized medicine in ... pharmaceutical industry, support from regulatory authorities, and emerging technologies ...
Breaking Biology Technology:House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 2Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 4Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 5Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 6Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 2Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 3Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 2Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 3
... SAN DIEGO, Oct. 28 Neurocrine Biosciences,(Nasdaq: NBIX ) announces the ... 2008 Financial Results, When: October 28, 2008 @ ... http://www.videonewswire.com/event.asp?id=52783 , How: Live over the Internet ... above., Contact: Claudia ...
... U.S. Phase II Study,of Nitazoxanide in Treatment-Naive Patients ... Oct. 28 Romark Laboratories, a privately,held biopharmaceutical ... at the upcoming 59th Annual Meeting of the ... also known as The,Liver Meeting(R), and the 50th ...
... Ziplex automated gene-expression system at the ... ... Molecular, a pioneer in,the development of cost-effective, easy-to-use gene-expression analysis,systems, ... L. Wittliff, a professor in the Department of,Biochemistry and Molecular ...
Cached Biology Technology:Romark Laboratories Announces Presentation of New Nitazoxanide Data at 59th Annual AASLD Meeting and 50th Anniversary IASL Meeting in San Francisco 2James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30 2James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30 3
(Date:9/18/2014)... in DNA, tiny strands of nucleic acid that contain ... this genetic data, our DNA is copied into RNA ... perform tasks in our cells. , Several years ... Unlike all other known RNAs, this molecule is circular, ... abundant, little has been known about how they are ...
(Date:9/18/2014)... ALEXANDRIA, Va.Sept. 18, 2014Many Americans across racial and ... the greatest impact on their day-to-day life, more ... memory, hearing and speech (57% of African-Americans, 49% ... of Hispanics). When asked which disease or ailment ... blindness ranked first among African-Americans followed by AIDS/HIV. ...
(Date:9/18/2014)... have devastated algal forests in the eastern Mediterranean Sea ... if their distribution continues to expand as the climate ... international team of researchers led by Dr Adriana Vergs ... Mediterranean Institute for Advanced Studies in Spain, is published ... the team surveyed more than 1000 kilometres of coastline ...
Breaking Biology News(10 mins):Scientists find how mysterious 'circular RNA' is formed, claim muscular dystrophy link 2Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3Tropical fish a threat to Mediterranean Sea ecosystems 2
... of life -- kids get sick and have fevers. ... but in some small children they spark convulsions called ... very upsetting to parents," said Robert L. Macdonald, M.D., ... something of an enigma, he said. The epilepsy research ...
... development of drug-resistant HIV when patients take as few as ... only when patients take most of their pills. These differences ... "fitness" of the drug-resistant HIV, say AIDS researchers in a ... MPH, an AIDS specialist at the University of California, San ...
... Vaccine and Infectious Disease Organization (VIDO) have developed a vaccine ... against a disease for which no vaccines are yet available. ... this vaccination technique may be effective against HCV. The study ... , The team, funded by the Canadian Institutes of Health ...
Cached Biology News:Molecular mechanism may explain how fevers spark seizures 2Molecular mechanism may explain how fevers spark seizures 3Viral 'fitness' explains different resistance patterns to aids drugs 2U of S researchers develop new vaccine candidate against hepatitis C 2
... EVO96R and Freedom EVO384R are fast multi-channel ... in three different sizes, with a multi-channel ... deliver significant time saving, with true parallel ... setup of your assays with the Freedom ...
... are fast multi-channel pipetting platforms consisting of ... with a multi-channel head and a robotic ... with true parallel processing of sample plates ... with the Freedom EVOware program. ...
Prepared by multi-temperature ethanol fractionation with extensive charcoal treatment and dialysis.• Assay: ≥ 95 % (HPCE)• pH ~5.2 (10 mg/mL in 0.15 M NaCl)...
... Linkage: This CLS number is a ... match Cornings product number. If showing ... the old Sigma-Aldrich number (Z71,405-4) or ... Dim: diam. 60 mm volume ...
Biology Products: