Navigation Links
Rapidly Synthesize Large Amounts of Capped RNA


Convenient format - premixed cap analog and nucleotides provided

Optimized for highest yield of capped RNA

80% capping efficiency

Complete with SUPERaseIn RNase inhibitor, DNase I, and positive control

Ideal for microinjection, in vitro or in vivo translation


The mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high yield transcription technology. Each reaction synthesizes 10-50 times the yield of capped RNA obtained with conventional in vitro transcription reactions.

The reaction is simple. DNA template is mixed with the reaction components and incubated at 37C for up to 2 hours. Figure 1 shows a time course experiment that plots yield of a 3.7 kb capped transcript over time. With this template, the mMESSAGE mMACHINE reaction is complete in only 30 minutes. In addition to synthesizing high yields of capped RNA transcripts (Figure 1), mMESSAGE mMACHINE reactions also produce full-length transcripts. Figure 2 shows an Agilent bioanalyzer 2100 electropherogram of the 3.7 kb transcript. The presence of a single, strong peak indicates the predominance of full length transcript. This is also evident in Figure 3, in which mMESSAGE mMACHINE reactions producing 1.7 kb, 3.7 kb and 9 kb transcripts were analyzed on a conventional denaturing agarose gel.

Figure 1. mMESSAGE mMACHINE Reaction Time Course. A 3.7 kb template was transcribed in 3 replicate mMESSAGE mMACHINE reactions as per protocol. After the indicated incubation times, each reaction was DNase treated as per protocol and a preliminary quantitatation was performed with RiboGreen (Molecular Probes). Each reaction was then purified using the MEGAclear Kit (Ambion) and quantitated by UV spectrophotometry.

Figure 2. Electropherogram of a mMESSAGE mMACHINE Reaction. A 3.7 kb template was used in a 20 l mMESSAGE mMACHINE reaction as per protocol and incubated for 30 min followed by analysis using the Agilent 2100 bioanalyzer.

Figure 3. Gel Analysis of mMESSAGE mMACHINE Reactions. Templates generating transcripts of sizes 1.7 kb, 3.7 kb and 9 kb were used in a mMESSAGE mMACHINE reaction as per protocol. One g of each reaction was denatured using NorthernMax-Gly Sample Loading Dye and analyzed on a 1% agarose gel with EtBr.


Cap analog is premixed with GTP at a ratio optimized for the best compromise between yield and proportion of capped transcripts (80%). The cap analog:GTP ratio can be adjusted with the GTP Solution provided in the kit to increase yields of long transcripts (>4 kb); in fact, RNA transcripts as long as 23 kb have been synthesized with mMESSAGE mMACHINE. The kit includes SUPERase In RNase Inhibitor . SUPERase In inhibits RNases A, B, C, 1 and T1 and offers the greatest available protection against RNases (see data). DNase I and a positive control DNA template are also included. The RNA synthesized from a mMESSAGE mMACHINE reaction is ideal for in vitro or in vivo translation studies.

Ambion also offers the mMESSAGE mMACHINE T7 Ultra Kit for the generation of RNA transcripts capped with Anti Reverse Cap Analog (ARCA). A base modification in this cap analog generates RNAs with ARCA incorporated only in the functional, translatable orientation, resulting in higher protein yields. The mMESSAGE mMACHINE T7 Ultra Kit includes reagents for both transcription and the addition of a poly(A) tail to capped transcripts.


back to top


Ordering Information
Cat# Product Name Size 1340 mMESSAGE mMACHINE SP6 Kit 25 rxns 1344 mMESSAGE mMACHINE T7 Kit 25 rxns 1345 mMESSAGE mMACHINE T7 ULTRA Kit 10 rxns 1348 mMESSAGE mMACHINE T3 Kit 25 rxns
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
2. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
3. Rapidly Prepare Probes and Markers for Small RNA Analysis
4. Synthesize High Yields of Biotinylated aRNA
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. PCR-Grade Nucleotides Larger Pack Sizes Now Available
7. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
8. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
9. Constructing Directional cDNA Libraries from Limited Amounts of RNA
10. Detecting Attomole Amounts of Small RNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Rapidly Synthesize Large Amounts Capped RNA

(Date:10/30/2014)... 30, 2014 Whitehouse Laboratories has formally ... Stability Testing and Package Test Capacity. Responding to growing ... a dramatic increase in environmental chamber storage space that ... and aging samples to be securely held on site ... capital expenditure has already taken place with the recent ...
(Date:10/30/2014)... , Oct. 30, 2014 Isis Pharmaceuticals, Inc. ... today announced that management will present a company overview at ... 8:30 a.m. ET in Boston, MA. ... available on the "Investors & Media" section of the Company,s ... the Isis website within 48 hours and will be archived ...
(Date:10/30/2014)... of the key challenges in the development of quantum ... , In a paper published today (28 October) in ... show how to make a new type of flexibly ... such as high-precision sensors and specialised superfast computers, often ... the methods for trapping these tiny particles are hugely ...
(Date:10/27/2014)... The new research report, “Gas ... Medium), Sub-type (Primary & Secondary), and End-User (Transmission ... & Power Generation) - Trends and Forecasts to ... analysis and forecasting of the market size. , ... 52 Figures spread through 146 Pages and in-depth ...
Breaking Biology Technology:Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 2Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 3Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 2Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 3Gas Insulated Switchgear Market worth $21,951.9 Million by 2019 - New Report by MarketsandMarkets 4
... AMSTERDAM, October 13 Amsterdam Molecular,Therapeutics ... gene therapy,announced today that the European Medicines Agency has ... for the treatment of,Duchenne muscular dystrophy. , ... (DMD) entitles,AMT to ten year market exclusivity in Europe ...
... HAIKOU CITY, China, Oct. 12 ... CPHI ), which develops,manufactures, and markets specialty pharmaceutical ... the Roth China Conference, to be held,October 12-14, 2009, ... participate in one-on-one meetings with conference attendees throughout,the three-day ...
... 12 Dr. Michael Merzenich , PhD ... brain plasticity expert and tech entrepreneur - was ... Washington, D.C. Also a member of the National Academy of ... scientists and doctors in the U.S. who have been selected ...
Cached Biology Technology:AMT Receives EMEA Orphan Drug Designation for Duchenne Muscular Dystrophy 2AMT Receives EMEA Orphan Drug Designation for Duchenne Muscular Dystrophy 3Dr. Michael Merzenich Joins Venerable Short List Honored for Cutting-Edge Neuroscientific Research 2Dr. Michael Merzenich Joins Venerable Short List Honored for Cutting-Edge Neuroscientific Research 3
(Date:10/30/2014)... UT Arlington bioengineering professor has received a $1.04 ... to regenerate cartilage tissue and reduce osteoarthritis using ... injection of microscaffolding made of biodegradable polymers., Liping ... the bioengineering department, said the research is primarily ... on the battlefield., "We,ve found that if we ...
(Date:10/30/2014)... Philadelphia, PA, October 30, 2014 – Bacteria in ... are critical for digestion. Yet, these same bacteria ... system if they penetrate the gut and enter ... natural response to protect the body, chronic or ... diseases. Prior research has established the involvement of ...
(Date:10/29/2014)... by unconventional oil and gas production are well over ... the open access journal Environmental Health . High ... The study is the first to be based on ... and could be used to supplement official air-quality monitoring ... that do not readily flow to the surface. This ...
Breaking Biology News(10 mins):UTA researcher uses microscaffolding injections to mend cartilage, prevent osteoarthritis 2Breakdown in gut barriers to bacteria may promote inflammation and craving in alcoholics 2Air quality and unconventional oil and gas sites 2Air quality and unconventional oil and gas sites 3
... Chicago, IllinoisJune 22, 2010"Seasick: Ocean Change and the ... in the US by the University of Chicago Press, ... in Environmental Reporting. Awarded by the Metcalf Institute for ... of the environment and recognizes reporting that has the ...
... puzzled over why a baby,s brain is particularly flexible ... have to learn a lot? A group of researchers ... Institute for Dynamics and Self-Organization in Gttingen, the Schiller ... put forward a new explanation: Maybe it is because ...
... (June 20, 2010) By emulating nature,s design principles, ... Engineering, Harvard Medical School and Dana-Farber Cancer Institute has ... be programmed to move and change shape on demand. ... highly suitable for medical applications because DNA is both ...
Cached Biology News:Growing brain is particularly flexible 2Researchers create self-assembling nanodevices that move and change shape on demand 2
... Cell Lines ,High Quality, Functionally-Validated, Ion Channel ... known for having a critical role in ... a key function in pain, CNS and ... have been investigated in therapeutic areas, such ...
Mouse polyclonal antibody raised against a partial recombinant CD320. NCBI Entrez Gene ID = 51293...
Mouse monoclonal antibody raised against a partial recombinant CHD8. NCBI Entrez Gene ID = CHD8...
Mouse monoclonal antibody raised against a partial recombinant DENR. NCBI Entrez Gene ID = DENR...
Biology Products: