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Rapidly Prepare Probes and Markers for Small RNA Analysis

mirVana Probe & Marker Kit


Ideal for analyzing miRNA, siRNA, and other small RNAs
Prepare high specific activity RNA or DNA probes
Includes purification system optimized for recovery of small RNA probes
Contains reagents to prepare Decade Markers and single-base ribonucleotide ladder, as well as DNA and RNA positive controls


The new mirVana Probe & Marker Kit provides a complete solution to quickly prepare and purify high specific activity radiolabeled RNA probes and small RNA markers such as Decade Markers (Figure 1). These tools are ideal for investigating a variety of small RNA molecules such as transfer RNA (tRNA), small nucleolar RNA (snoRNA), small nuclear RNA (snRNA), small interfering RNA (siRNA), and micro RNA (miRNA).

The purification system included in the kit allows fast and efficient clean up of 5' end labeled probes. The Purification Cartridges can also be used to clean up small probes prepared by in vitro transcription (e.g. with the mirVana miRNA Probe Construction Kit, see Figure 1). Unlike RNA probes prepared by in vitro transcription, 5' labeled probes generally do not require gel purification prior to use in a sensitive assay such as solution hybridization.
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