Navigation Links
Rapid hit confirmation and early hERG liability testing using the IonWorks HT system

IONWORKS HT APPLICATION NOTE #4

By James Costantin, Ph.D., Yen-Wen Chen, Ph.D., Jinfang Liao, Ph.D., Jeff Quast, Andrew Wittel, Shawn Handran, Ph.D. and Naibo Yang, Ph.D. Molecular Devices Corporation, 3280 Whipple Road, Union City, CA 94587.


INTRODUCTION
Voltage-dependent ion channels represent a largely untapped portion of the potential drug target market.1 Until recently, voltage-dependent ion channels have been studied during the drug discovery process either using indirect methods of measuring the presence or activity of ion channels or by the gold standard patch clamp technique. Some of the higher throughput, lower content techniques include binding and flux assays, and the use of fluorescent dyes.2 While these assays have proven useful, they lack the ability to maintain a population of ion channels in a particular conformational state due to the lack of voltage control. The traditional patch clamp technique is labor intensive but offers direct control of the membrane potential and a direct measurement of ionic current. Thus, a population of channels can be driven to a particular conformational state, which may affect drug binding. The low throughput conventional patch clamp technique creates a severe bottleneck in following up on the hits from upstream indirect ion channel assays.3

This application note illustrates how the IonWorks HT system can be used to rapidly confirm hits from higher-throughput assays as well as perform earl
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Rapidly Synthesize Large Amounts of Capped RNA
Post Your Comments:
(Date:8/21/2014)... OTC Markets Group Inc. ... company, on its approval to list on The ... the best marketplace for established U.S. and global ... Logo - http://photos.prnewswire.com/prnh/20110118/MM31963LOGO "We ... its growth strategy and achievement of this important ...
(Date:8/21/2014)... August 21, 2014 Pursuit Solutions ... optimization technology and services for Life Sciences sales ... Pharma’s Use of New Commercial Sales Models” by ... Analytics. , The article examines some innovative models ... are being tested in the pharmaceutical market. Robinson ...
(Date:8/21/2014)... Boston, MA (PRWEB) August 21, 2014 ... collaboration company, has been recognized as one of ... 500 | 5000 magazine for the 2nd consecutive ... on its 33rd annual Inc. 500|5000, an exclusive ... list represents the most comprehensive look at the ...
(Date:8/20/2014)... inventions licensed to biotechnology firms has revealed early ... these roadblocks, the researchers suggest that better communication ... could lead to faster commercialization down the road. ... made in university laboratories and licensed to biotechnology ... which have a high failure rate. But a ...
Breaking Biology Technology:OTC Markets Group Congratulates Immune Pharmaceuticals on NASDAQ Listing 2Pursuit's Peter Robinson Publishes "Grading Pharma's Use of New Commercial Sales Models" 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3Early bottlenecks in developing biopharmaceutical products delay commercialization 2Early bottlenecks in developing biopharmaceutical products delay commercialization 3
... , NINGBO, China , ... Equipment Co., Ltd. (TAIJI),s FGD (Flue-gas desulfurization),technology was officially ... For the project, TAIJI chose to use steel slag ... used in desulfurization. In,comparison to the use of lime ...
... Mass. , May 18 , ... What: Virtify, ... solutions, will co-host the webinar, "Managing Paper at Its Roots: Extending ... how emerging ECC solutions are accelerating traditional document preparation by compressing ...
... HALMSTAD, Sweden , May 18, 2010 , ... - With Photo , ... The Redsense safety device is now cleared by FDA for Home/Self ... is designed to detect venous needle dislodgement,during hemodialysis and its use has already improved in-clinic ...
Cached Biology Technology:TAIJI Announces Official Operation of Its Sintering FGD Project for Tangshan 2Webinar Alert: 'Managing Paper at Its Roots: Extending Beyond Document Management to Enterprise Content Compliance' 2Redsense Medical Receives FDA Clearance for the Redsense Safety Device for Home/Self use During Home Hemodialysis 2
(Date:8/21/2014)... the journal Carcinogenesis by researchers at the ... protein adenomatous polyposis coli (APC) in suppressing colorectal cancer ... U.S. , Lead author Kristi Neufeld, associate professor in ... Cancer Biology program at the KU Cancer Center, has ... understand the various activities of APC, a protein whose ...
(Date:8/21/2014)... successfully replicated one of the crucial steps in photosynthesis, ... which could manufacture hydrogen as a fuel. , "Water ... exciting prospect to use them to create hydrogen, and ... from the ARC Centre of Excellence for Translational Photosynthesis ... offers potential as a zero-carbon replacement for petroleum products, ...
(Date:8/20/2014)... 2014 George E. Fox, a John and Rebecca Moores ... (UH), was named a fellow in the International Society for ... Fox is one of four members two from the ... as fellows in 2014. Fellows are elected every three years, ... 500 members from more than 20 countries, the ISSOL includes ...
Breaking Biology News(10 mins):Research offers insight into cellular biology of colorectal cancer 2Research offers insight into cellular biology of colorectal cancer 3Water and sunlight the formula for sustainable fuel 2UH professor named fellow by International Astrobiology Society 2
... America. Their geographical distribution extends from the Orinoco River ... the Paran (Argentina) to the South. Over this whole ... have recorded 28 carnivorous species of these fish (2). ... of fish, the mechanisms that generated the species richness ...
... appearing in the Dec. 2007 Journal of Lipid Research ... Affect Diet-Associated Cholesterol Metabolism, Crohns Disease Increases Progression of ... , Genetic Variants Affect ... variants in two metabolic genes alter how the body ...
... WHAT: The 2007 American College of Neuropsychopharmacology Annual ... and behavior from the worlds leading scientists. Presentations ... post-traumatic stress disorder, autism and addiction. ... Link between Cancer and Schizophrenia A series of studies ...
Cached Biology News:Present-day species of piranha result from a marine incursion into the Amazon Basin 2Present-day species of piranha result from a marine incursion into the Amazon Basin 3Story ideas from the Journal of Lipid Research 2Story ideas from the Journal of Lipid Research 3New studies on schizophrenia, depression, trauma and autism highlight annual meeting 2New studies on schizophrenia, depression, trauma and autism highlight annual meeting 3
... variety of environmental and pathophysiological stressful conditions ... proteins known as heat shock proteins (HSPs), ... (SRPs). The Hsp90 family of proteins ... and beta, Grp94, and Trap-1 (Hsp75). It ...
... (AR) Competitor Assay Kits provide a sensitive ... potential AR ligands using fluorescence polarization (FP). ... domain protein tagged with His and GST ... androgen ligand (Fluormone AL Green in the ...
Custom LNA Oligonucleotide synthesis ,High quality Custom LNA Oligonucleotides are available for a variety of different specialty applications and innovative products. ,Please inquire for further...
... - with a modular approach to instrument ... ChemStation Plus NDS family provides modular software ... data management, giving you the flexibility to ... needs now and that can grow as ...
Biology Products: