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Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS

ectly into LC/MS system without derivatization, evaporation, and reconstitute steps. The 96-well format Focus can be used for automation and high-throughput screening.

Only two product ions were used for this analysis because amphetamine and methamphetamine only give two intense product ions (Figure 4, Table 2) while ephedrine, MDA, MDMA, and MDEA produce multiple intense product ions (Table 2). Run-to-run retention time is very reproducible with a <1.4% RSD. Two product ions with a retention time match can be strong evidence for positive identification of amphetamine drugs (Figure 2). Both the urine double blank and the blank with IS show no interference of the analysis at low quantitation level (LQL). For the standard calibration curve, the LQL is 5 ng/mL and upper quantitation level (UQL) is 1000 ng/mL. This LC/MS/MS method is very sensitive and can be possibly adapted to other body fluid analysis for amphetamines, such as sweat and oral fluid which have confirmatory drug cutoff levels of 25 ng/mL and 50 ng/mL, respectively.

Conclusion

The LC/MS/MS method described in this application note is simple and sensitive. This method can quantitatively analyze amphetamine drugs at 50 times below the drug cutoff levels in urine. The Varian SPE and 1200L LC/MS/MS system demonstrated excellent performance for the urinalysis of amphetamines. The system can be a useful tool for forensics, clinical, and doping laboratories.

Reference

1. http://workplace.samhsa.gov/ResourceCenter/DT/FA/ GuidelinesDraft4.htm


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