Navigation Links
Rapid Screening of Amphetamine Drugs in Urine by Positive Ion Electrospray LC/MS/MS

ectly into LC/MS system without derivatization, evaporation, and reconstitute steps. The 96-well format Focus can be used for automation and high-throughput screening.

Only two product ions were used for this analysis because amphetamine and methamphetamine only give two intense product ions (Figure 4, Table 2) while ephedrine, MDA, MDMA, and MDEA produce multiple intense product ions (Table 2). Run-to-run retention time is very reproducible with a <1.4% RSD. Two product ions with a retention time match can be strong evidence for positive identification of amphetamine drugs (Figure 2). Both the urine double blank and the blank with IS show no interference of the analysis at low quantitation level (LQL). For the standard calibration curve, the LQL is 5 ng/mL and upper quantitation level (UQL) is 1000 ng/mL. This LC/MS/MS method is very sensitive and can be possibly adapted to other body fluid analysis for amphetamines, such as sweat and oral fluid which have confirmatory drug cutoff levels of 25 ng/mL and 50 ng/mL, respectively.

Conclusion

The LC/MS/MS method described in this application note is simple and sensitive. This method can quantitatively analyze amphetamine drugs at 50 times below the drug cutoff levels in urine. The Varian SPE and 1200L LC/MS/MS system demonstrated excellent performance for the urinalysis of amphetamines. The system can be a useful tool for forensics, clinical, and doping laboratories.

Reference

1. http://workplace.samhsa.gov/ResourceCenter/DT/FA/ GuidelinesDraft4.htm


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
11. Rapidly Prepare Probes and Markers for Small RNA Analysis
Post Your Comments:
(Date:1/15/2014)... January 15, 2014 Cynvenio, a cancer ... through the genomic analysis of tumor cells in the ... a web-based cancer decision support application powered by CollabRx, ... Cynvenio’s ClearID™ genomic test, Therapy Finder will provide breast ...
(Date:1/15/2014)... A study has been launched to test whether technology ... help to tackle the problem of obesity.  This unique healthcare ... based in Stowmarket) and academics at University Campus Suffolk is ... which is inspired by equipment used to collect data about ...
(Date:1/15/2014)... NC (PRWEB) January 15, 2014 DTS ... to its Online Web Portal for Life Science organizations who ... to specify the subject matter of their documents in advance ... will help reduce time-to-delivery of translations, often a critical factor ...
(Date:1/15/2014)... , Jan. 15, 2014 TaiGen Biotechnology Company, ... exclusive agreement with R-Pharm, a leading Russian pharmaceutical company, ... the Russian Federation , ... Independent States (CIS). Nemonoxacin is a novel antibiotic for the ...
Breaking Biology Technology:Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4
... Hi-Bred Biotechnology Leadership ... at Beijing Ag Summit, ... public and private sectors will help increase,farm productivity in the country ... vice president -- DuPont Crop,Genetics Research and Development, told attendees at ...
... 25 IDEXX,Laboratories, Inc. (Nasdaq: IDXX ), today ... 18% to $249.1 million from $211.2 million,for the first ... the,quarter ended March 31, 2008 were $0.43, compared to ... adjusted diluted EPS for the first,quarter grew 21% to ...
... (Nasdaq: KOSN ) will announce its first quarter ... close., The announcement of Kosan,s first quarter 2008 ... live webcast on May 1, 2008 at 1:30,p.m. Pacific ... live,call by dialing 888.680.0878 (US) or 617.213.4855 (international), access,code ...
Cached Biology Technology:DuPont Partnering with China to Increase Farm Productivity 2DuPont Partnering with China to Increase Farm Productivity 3IDEXX Laboratories Announces First Quarter Results 2IDEXX Laboratories Announces First Quarter Results 3IDEXX Laboratories Announces First Quarter Results 4IDEXX Laboratories Announces First Quarter Results 5IDEXX Laboratories Announces First Quarter Results 6IDEXX Laboratories Announces First Quarter Results 7IDEXX Laboratories Announces First Quarter Results 8IDEXX Laboratories Announces First Quarter Results 9IDEXX Laboratories Announces First Quarter Results 10IDEXX Laboratories Announces First Quarter Results 11IDEXX Laboratories Announces First Quarter Results 12IDEXX Laboratories Announces First Quarter Results 13IDEXX Laboratories Announces First Quarter Results 14IDEXX Laboratories Announces First Quarter Results 15IDEXX Laboratories Announces First Quarter Results 16IDEXX Laboratories Announces First Quarter Results 17Kosan Announces May 1, 2008 Conference Call and Webcast of First Quarter 2008 Financial Results 2
(Date:4/17/2014)... and Professor Luca Razzari of the nergie Matriaux ... grants from the John R. Evans Leaders Fund ... the acquisition of state-of-the-art biotech and nanophotonics equipment. ... from the Ministre de l,Enseignement suprieur, de la ... (MESRST). These new laboratories will help us develop ...
(Date:4/17/2014)... engineer has developed a patented technique that improves ... The same technique could help police during drug ... Kay L. Theede chair in engineering and department ... research team have created a template-based system that ... The distance detection method called stand-off bomb ...
(Date:4/17/2014)... is available in German . ... the protection money demanded of him, he can expect his ... however, as fear of the consequences is enough to make ... parasitic birds, which lay their eggs in other birds, nests. ... brood parasites take their revenge by destroying the entire nest. ...
Breaking Biology News(10 mins):New state-of-the-art biotech and nanotech equipment for INRS 2Patented research remotely detects nitrogen-rich explosives 2Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3
... the Keck School of Medicine of the University of ... estrogen, the female sex hormone, activates genes in breast-cancer ... to eventually lead to new treatments for the disease. ... of the Keck School,s Department of Biochemistry and Molecular ...
... for rare and neglected diseases that include potential treatments ... virus that affects the central nervous system of newborns, ... and a rare lung disease. The six new projects ... for Rare and Neglected Diseases (TRND) program. "These ...
... Nov. 15, 2011 The Los Angeles World Airports (LAWA), ... has awarded a contract modification to Unisys (NYSE: ... system, used to identify the 45,000 airport employees, contractors, police ... The work is an early step in LAWA,s plan ...
Cached Biology News:USC researchers discover key aspect of process that activates breast cancer genes 2NIH TRND program announces next round of drug development projects 2NIH TRND program announces next round of drug development projects 3NIH TRND program announces next round of drug development projects 4NIH TRND program announces next round of drug development projects 5Los Angeles World Airports Selects Unisys to Upgrade ID Card Reader System and Network 2Los Angeles World Airports Selects Unisys to Upgrade ID Card Reader System and Network 3
... boxes are ideal for use in refrigerators, freezers, or ... Four vibrant colors allow for color coding to project, ... nitrogen drainage or promote cool air circulation. Supplied with ... 81 tubes. Box dimensions: 13 x 13 ...
RABBIT ANTI BACTERIAL HISTIDASE...
2,4-Dinitrophenyl hapten is conjugated to BSA (Bovine Serum Albumin) protein through lysine....
See product name for description....
Biology Products: