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Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

typing as an alternative to phenotyping for determination of metabolic status in a clinical trial setting. AAPS Pharmsci 2(4):1-11 (2000).

2. Marez, D., Legrand, M., Sabbagh, N., Lo Guidice, J.M., Spire, C., Lafitte, J.J., Meyer, U.A. and Broly, F. Polymorphism of the cytochrome P450 CYP2D6 gene in a European population: characterization of 48 mutations and 53 alleles, their frequencies and evolution. Pharmacogenomics 7:193-202 (1997).

3. Daly et al. Nomenclature for human CYP2D6 alleles. Pharmacogenetics 6:193-201 (1996)

4. Chen, Z.R., Somogyi, A.A. and Bochner, F. Simultaneous determination of dextromethorphan and three metabolites in plasma and urine using high-performance liquid chromatography with application to their disposition in man. Ther Drug Monit 12:97-104 (1990).

5. Hersberger, M, Marti-Jaun, J, Rentsch, K and Hanseler, E. Rapid detection of the CYP2D6*3, CYP2D6*4, CYP2D6*6 alleles by tetra-primer PCR and of the CYP2D6*5 allele by multiplex long PCR. Clinical Chemistry 46(8):1072-1077 (2000).

6. De La Vega, F.M., Dailey, D., Ziegle, J., Williams, J., Madden, D. and Gilbert, D.A. New generation pharmacogenomic tools: a SNP linkage disequilibrium Map, validated SNP assay resource, and high-throughput instrumentation system for large-scale genetic studies. BioTechniques 32:548-554 (2002).


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