Navigation Links
Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

lor real-time protocol on the DNA Engine Opticon 2 system. The protocol uses two hydrolysis probes labeled with two different fluorophores, FAM and VIC, to distinguish between the wild-type G nucleotide and the variant A nucleotide at position 1934 of the CYP2D6 gene. After completion of the assay, each sample was automatically assigned to one of three groups, homozygous GG, homozygous AA, or heterozygous GA, by an analysis feature of the Opticon Monitor software.

This assay is a valuable tool for pharmacogenomics, in which genotype information can be used to design the most effective drug therapy for an individual patient. The short time (total of 3 hours) required for the qPCR run and data analysis, and the ability to perform reactions in a 96-well format should allow this assay to be used for routine genotyping in a clinical setting. In our patient population, the nine patients who carried two copies of the variant allele were classified as poor metabolizers. For these patients, a poorly metabolized drug could be avoided, or the dose and course of drug administration could be reduced, to avoid any adverse effects that might occur with a standard course of drug therapy. The ability of the Opticon 2 system to unequivocally distinguish between the three SNP genotypes in both the control and the patient DNA samples, and the availability of TaqMan assays for SNP analysis of other alleles in the CYP2D6 gene and other genes, demonstrate that the Opticon 2 system is an effective platform for a wide range of SNP genotype assays.6


References
1. McElroy, S., Sachse, C., Brockmoller, J., Richmond, J., Lira, M., Friedman, D., Roots, I., Silber, B. M. and Milos, P. M. CYP2D6 geno
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
11. Rapidly Prepare Probes and Markers for Small RNA Analysis
Post Your Comments:
(Date:9/2/2014)... (PRWEB) September 02, 2014 The Little ... southwestern Chicagoland, constructed a new 290,000 square-foot West Pavilion ... Tufftec® Lockers from Scranton Products for the nurse’s ... 1930, Little Company of Mary Hospital’s $180 ... Summer 2014 with the connection of the West Pavilion ...
(Date:9/2/2014)... a billionth of a meter in size, are around ... benefit human health, as in some innovative early cancer ... viruses, air pollution, traffic emissions, cosmetics, sunscreen and electronics. ... St. Louis, led by Lan Yang, PhD, the Das ... Engineering, and their collaborators at Tsinghua University in China ...
(Date:9/2/2014)... YORK , September 2, 2014 ... Study on Membrane Technology in Pharmaceutical, Biopharma and Life ... Highest Growth by 2019" the global membrane technology market for pharmaceutical, ... million in 2014 and is expected to grow at ... reach an estimated value of USD 10,886.0 million in ...
(Date:9/2/2014)... 2014 Opertech Bio, Inc., a ... cost effective approach to sensory evaluation, today announced ... the U.S. Patent and Trademark Office, encompassing high-throughput ... , “Opertech’s taste evaluation technology called Microtiter Operant ... throughput system for rapid characterization of taste sensory ...
Breaking Biology Technology:Tufftec® Lockers from Scranton Products Selected by Little Company of Mary Hospital Near Chicago to Create an Uplifting Environment 2Tufftec® Lockers from Scranton Products Selected by Little Company of Mary Hospital Near Chicago to Create an Uplifting Environment 3Engineers develop new sensor to detect tiny individual nanoparticles 2Engineers develop new sensor to detect tiny individual nanoparticles 3Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 2Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 3Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 4Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 5Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2
... , QUEBEC CITY , Jan. 22 ... TSX: AEZ) (the "Company"), a late-stage drug development company specialized ... 21, 2010 , the Company received a letter from the ... the minimum closing bid price of its common shares had ...
... , ... Bioamber,( http://www.bio-amber.com/ ) , a joint venture between US-based DNP,( ... )(Agro-industrie Recherches et Développements),announces the successful start-up and commissioning of ... , the plant has been,producing renewable succinic acid from wheat ...
... ... contouring, will be presented at the upcoming ICAD & 3rd ECAA Asia conference. Dr. ... Y. Yu will present his latest clinical data showing successful reduction of acne using TriPollar. ... (PRWEB) January 20, ...
Cached Biology Technology:Æterna Zentaris Receives NASDAQ Notification Related to Minimum Bid Price 2Æterna Zentaris Receives NASDAQ Notification Related to Minimum Bid Price 3Bioamber Commissions World's First Renewable Succinic Acid Plant 2Bioamber Commissions World's First Renewable Succinic Acid Plant 3TriPollar Technology Clinically Proven to Significantly Reduce Stretch Marks and Acne 2TriPollar Technology Clinically Proven to Significantly Reduce Stretch Marks and Acne 3
(Date:9/2/2014)... new Journal of Evolutionary Biology study ... can influence language diversification. , Investigators argue that ... biodiversity in the Galpagos Islands is also responsible ... the natural oceanic barriers that impede interaction between ... from one another due to a reduction of ...
(Date:9/2/2014)... that professional baseball pitchers with poor core stability are more ... season because of injury than are pitchers who have good ... In the study, 347 pitchers were assessed for lumbopelvic control ... as they raised a leg to step up were up ... days cumulative, not consecutive during the season than ...
(Date:9/2/2014)... is considered the most ideal source of nutrition ... critical role in the evolution and civilizations of ... contains a large number of bacterial species, including ... as no surprise to scientists and physicians. , ... to be the result of co-evolutionary and co-adaptive ...
Breaking Biology News(10 mins):In pro baseball pitchers, weak core linked to more missed days 2In pro baseball pitchers, weak core linked to more missed days 3Are human breast milk microbiome 'neutral'? 2Are human breast milk microbiome 'neutral'? 3
... of 1000, the leading scientific evaluation service, has highlighted research ... Birds use plumage colour to recognize and select potential mates. ... a striking colour change, as demonstrated by two closely related ... in the American Naturalist -- selected and reviewed ...
... the possibility that flies and other insects that encounter nanomaterial ... could pick up and transport nanoparticles on their bodies, transferring ... The study on carbon nanoparticles barely 1/5,000th the width ... issue of ACS, Environmental Science & Technology , a ...
... an advance that could help ease the antibiotic drought, ... test that enlists pinhead-sized worms in efforts to discover ... ACS Chemical Biology , a monthly journal. ... that existing methods for identifying germ-fighting drugs involve adding ...
Cached Biology News: