Navigation Links
Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System

ood samples. A total of 211 patient DNA samples were genotyped in duplicate, along with the GG and AA homozygous control DNA, GA heterozygous control DNA, and a no-template control. The data were then analyzed by plotting the C(t) value in channel 1 (FAM) against the C(t) value in channel 2 (VIC) for each sample, using the Opticon Monitor software. The data for a 96-well plate are shown in Figure 2. Four tightly clustered groups were generated. Group 1 (blue) contained samples homozygous for the G allele, group 2 (red) contained samples homozygous for the A allele, group 3 (green) contained samples that were GA heterozygotes and group 4 (orange) were no-template control (NTC) reactions.

The results of genotyping the patient samples are summarized in Table 3. Of the 211 samples, 153 patients (72.5%) were homozygous for the wild-type allele G, while 9 (4.3%) were homozygous for the variant allele A. The remaining 49 samples (23.2%) were heterozygotes. The patients with a heterozygous genotype containing one copy of the wild-type allele would be extensive metabolizers, while those with the homozygous AA genotype would be poor metabolizers. The genotypes were present at frequencies consistent with previous studies.5

The results of the real-time qPCR assay were confirmed by restriction enzyme digestion of the PCR product at a BstN1 site that spans the SNP site. For each of the 211 patient samples, the CYP2D6*4 genotype determined by the real-time qPCR assay was identical to that determined by restriction digestion (data not shown).


Discussion
In this study, we accurately genotyped 211 patient samples for the CYP2D6*4 allele using a 2-co
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
11. Rapidly Prepare Probes and Markers for Small RNA Analysis
Post Your Comments:
(Date:1/22/2015)... Rootstown, OH (PRWEB) January 22, 2015 Crystal ... announced today that it has received AOAC-PTM Certifications for the ... O111, O121, and O145; collectively referred to as STEC or ... at 1 colony forming unit (cfu) per 325 g of ...
(Date:1/22/2015)... MA (PRWEB) January 22, 2015 zFlo ... is pleased to announce an exciting product from a new ... motion analysis solutions over the past 15 years, with offerings ... Most recently, their 3D IMU system (inertial measurement unit), iSen, ...
(Date:1/22/2015)... DE (PRWEB) January 22, 2015 Controlled ... Pistoia Alliance, which helps companies check the legal requirements ... to expand to China. , As their international ... are globally adopting software solutions built as a result ...
(Date:1/22/2015)... Denville, NJ (PRWEB) January 22, 2015 ... shearing technologies such as the Bioruptor® and complete ... semi-automation system for chromatin immunoprecipitation, alleviating the need ... reagents and controls needed for ChIP of histones ...
Breaking Biology Technology:Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 23D Motion Capture Just Got Easy 2Global Compliance Service for Controlled Substances to Expand to China 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... 21, 2012 International scientific and government experts ... potential use of Oxitec,s genetically modified Aedes aegypti ... The independent forum was co-organised by the Smithsonian ... Studies and the University of Panama. This brought together national ...
... ROCKVILLE, Md., May 21, 2012  Neuralstem, Inc. (NYSE ... President, will present at the World Stem Cells ... ) on Tuesday, May 22nd at 12:30 PM. ... Disorders," will review Neuralstem,s cellular therapy trial in ...
... 18, 2012 Champions Oncology Inc. (OTC: CSBR) announced ... Amendment (CLIA) certification from the Maryland Department of Health ... for the company to conduct medical laboratory tests. ... "Obtaining CLIA approval is a significant milestone for our ...
Cached Biology Technology:Expert Meeting Discusses Testing of Oxitec Transgenic Mosquitoes in Panama 2Expert Meeting Discusses Testing of Oxitec Transgenic Mosquitoes in Panama 3Neuralstem CEO to Present at the World Stem Cells and Regenerative Medicine Congress in London 2Champions Oncology Inc. Receives Approval from U.S. Regulatory Authorities 2Champions Oncology Inc. Receives Approval from U.S. Regulatory Authorities 3
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ... "Micro Market Monitor: North America Perimeter Security Systems Market" ... The North American perimeter security market is ... to 2019. Although the U.S. market holds a larger share ...
(Date:12/17/2014)... Markets ( http://www.researchandmarkets.com/research/s6qzt5/samsung_galaxy_s5 ) has announced the ... Button Synaptics Fingerprint Sensor Technology Analysis" report to ... Based on a totally different sensing technology ... time a fingerprint sensor in its product. ...
(Date:12/17/2014)... 2014 Valencell, a leader in performance biometric data ... industry leaders such as Intel, Jabra, Atlas, and Scosche ... products. These products will be showcased at CES 2015, ... . "Our partners choose PerformTek ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2
... survey of employers in the landscape industry revealed ... with technical knowledge, practical application, and problem-solving skills. ... landscape management decisions is crucial to their career ... over the past two decades, tools are now ...
... WCC Smart Search & Match,the leading provider of ... Lion has joined the company as Product Marketing ... than 15 years of product and marketing management,experience ... efforts,for many high-tech products and solutions worldwide. He ...
... atomic structure of a powerful "molecular motor" that packages ... their assembly, an essential step in their ability to ... from Purdue University and The Catholic University of America, ... works. Parts of the motor move in sequence like ...
Cached Biology News:Web-based case studies help students develop career skills 2Biologists learn structure, mechanism of powerful 'molecular motor' in virus 2Biologists learn structure, mechanism of powerful 'molecular motor' in virus 3
... DTX Series Multimode Detectors provide unmatched ... of application and automation needs. Capable ... measurements on the same instrument, the ... instruments or can be integrated seamlessly ...
BioGenex IHC Kits for Rodent Tissues are designed for immunohistochemistry on rodent and rabbit tissues that are closely related to the source of the primary antibody. This kit comes with rat seconda...
... Solution is available in a ready-to-use ... reagent for dewaxing paraffin-embedded tissue sections ... hybridization, or other analytical methods. The ... of dipping the slides in a ...
... The BioGenex Super Sensitive ... is a novel product ... non-biotin polymeric technology. This ... signal to noise ratio ...
Biology Products: