( Superior ...)Rapid PCR* Using Active Tube Temperature Control On Thermo's PCR Machines

Superior accuracy of PCR protocols run under Active Tube Control allows reduction of dwell times.

Due to the results obtained in the standard block controlled protocol (figure 3), we can confidently assume that it would be possible to successfully amplify these samples with a much shorter dwell time by using the more accurate Active Tube Control (figure 5).

As can be seen in figure 5, the Active Tube Control successfully allowed the reduction in dwell times and, hence, total run time, without a comprise on yield quality for both the Px2 and MBS instruments.

Conclusions

The accuracy and yield of a PCR is dependant on the accuracy of hitting and maintaining a precise target temperature. In Block Control, the only method available to ensure that the sample has reached the target temperature is to lengthen dwell times. In contrast, Active Tube Control measures the actual sample temperature allowing the use of shortened dwell times. Using these short dwell times on the Px2 and MBS allowed completion of a 3 temperature PCR protocol in less than 1 hour.

*The Polymerase Chain Reaction (PCR) is covered by U.S. patents 4683195, 4683202, and 4965188, which are owned by Hoffman-La Roche Ltd.

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