Navigation Links
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

re analyzed by electrophoresis on a Criterion Tris-HCl 415% gradient polyacrylamide gel.


Results

The elution profile during each run was monitored at 280 nm, and a series of 1 ml fractions was collected using a BioFrac fraction collector.

The resulting chromatograms obtained with the 75, 100, 250, 500, and 750 mM imidazole elution buffers were superimposed using the Trace Compare feature provided within the BioLogic DuoFlow software; these are illustrated in Figure 3.

The data in Figures 2 and 3 indicate that optimal results were obtained with an elution buffer consisting of 250 mM imidazole, 50 mM potassium phosphate, 300 mM NaCl, pH 8.0. This elution buffer provided the most effective separation and removal of the impurities (Figure 2, lanes 5 and 6, respectively).


Conclusions
The development of rapid and efficient methods for the chromatographic isolation and characterization of biomolecules requires a chromatography system that is capable of advanced automated operation with minimal user intervention. This study demonstrates that the sophisticated valve configuration, high degree of flexibility, and versatile software of the BioLogic DuoFlow system make it ideal for meeting the demands of a modern method development laboratory.


Coomassie is a trademark of BASF Aktiengesellschaft.

Information in this tech note was current as of the date of writing (2004) and not neccessarily the date this version (Rev A, 2005) was published.


back to top
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
11. Rapidly Prepare Probes and Markers for Small RNA Analysis
Post Your Comments:
(Date:10/18/2014)... According to a new market research report ... Global Trends & Forecasts up till 2017”, hydraulic fracturing ... This value is expected to increase from $40 billion ... CAGR during the same period. North America is expected ... terms of hydraulic horse power supplied by the year ...
(Date:10/18/2014)... The “Global Diagnostic Electrocardiograph (ECG) ... End-user (Hospitals, Home/Ambulatory, Research Center, Physician Office, Nursing ... Forecast to 2020” provides a detailed overview of ... trends, and strategies impacting the global diagnostic ECG ... the revenue and share analysis. , The global ...
(Date:10/17/2014)... looking-glass to Wonderland, mirrors in the real world can ... new class of mirror that works like no other. ... (OSA) new high-impact journal Optica , ... type of mirror that forgoes a familiar shiny metallic ... unusual magnetic property of a non-metallic metamaterial. , ...
(Date:10/17/2014)... (PRWEB) October 17, 2014 For ... top employers, as published in today’s print issue ... Careers’ annual Top Employers Survey polled employees in ... the 20 best employers. Rankings were determined ... asked to rate their companies on 23 characteristics, ...
Breaking Biology Technology:Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 2Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 3Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 4The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 2The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 3The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 4The Electrocardiograph (ECG) Market Is Expected to Reach $5,435 Million By 2020 - New Research Report by MarketsandMarkets 5Magnetic mirrors enable new technologies by reflecting light in uncanny ways 2Magnetic mirrors enable new technologies by reflecting light in uncanny ways 3Magnetic mirrors enable new technologies by reflecting light in uncanny ways 4DuPont Named Top Employer by Science Magazine 2DuPont Named Top Employer by Science Magazine 3
... Advanced Cell Technology, Inc. ("ACT"; OTCBB: ACTC), a ... that it has filed a clinical trial application (CTA) ... (MHRA) seeking clearance to initiate its Phase 1/2 clinical ... human embryonic stem cells (hESCs) to treat patients with ...
... Calif., April 20, 2011 Clontech Laboratories, Inc., ... announced the release of the iDimerize inducible ... ARGENT™ technology developed by ARIAD Pharmaceuticals, ... systems enable precise, real-time control of protein-protein interactions ...
... Practice Fusion, the leading EHR and healthcare community ... Paul Willard as Vice President of Marketing and Liz ... of the expanded executive team follows a $23 million ... Health Record system , led by Founders Fund earlier ...
Cached Biology Technology:ACT Files European Clinical Trial Application for Phase 1/2 Study Using Embryonic Stem Cells to Treat Macular Degeneration 2ACT Files European Clinical Trial Application for Phase 1/2 Study Using Embryonic Stem Cells to Treat Macular Degeneration 3ACT Files European Clinical Trial Application for Phase 1/2 Study Using Embryonic Stem Cells to Treat Macular Degeneration 4Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems 2Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems 3Announcement - Clontech Laboratories, Inc. Releases the iDimerize™ Cell Signaling and Expression Systems 4Practice Fusion Expands Executive Team with New VP of Marketing and Senior Business Development Director 2Practice Fusion Expands Executive Team with New VP of Marketing and Senior Business Development Director 3Practice Fusion Expands Executive Team with New VP of Marketing and Senior Business Development Director 4
(Date:10/16/2014)... wages war on the human body. Battles are ... In pancreatic cancer, this stalemate—known as tumor dormancy—can ... malignant, a phenomena that is poorly understood. ... laboratory of Salvatore Torquato, a Professor of Chemistry ... surrounding tumor dormancy and the switch to a ...
(Date:10/16/2014)... a new synthesis of recent research findings to inform ... the two states. , The Ecology and Management ... A Synthesis of the Relevant Biophysical Science and Implications ... by the U.S. Forest Service,s Pacific Northwest Research Station, ... for a synthesis of the large body of scientific ...
(Date:10/16/2014)... monocultures can be cultivated efficiently, they are anything ... caused by monoculture cultivation is becoming increasingly evident. ... crop form and are regarded as the sole ... – quite wrongfully, finds Bernhard Schmid, an ecology ... a novel form of agriculture and forestry. After ...
Breaking Biology News(10 mins):Modeling tumor dormancy 2New report synthesizes best available science on management of moist mixed-conifer forests 2Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3
... government builds on its $1 billion investment to clean up ... developed innovative new maps of both environmental threats and benefits ... world,s largest fresh water resource. Writing this week (Dec. ... of Science ( PNAS ), a group led by ...
... with pollutants from Asia, transpacific dust plumes deliver vast ... manuscript published online ahead of print in the journal ... detected thousands of unique microbial species, many of which ... David J. Smith, a graduate student at the University ...
... common reed, grows rapidly into dense stands of tall plants ... Early treatment is the key to controlling Phragmites . ... they take over their environment if we don,t know where ... from Michigan Technological University,s Michigan Tech Research Institute (MTRI), the ...
Cached Biology News:Mapping effort charts restoration tack for Great Lakes 2Mapping effort charts restoration tack for Great Lakes 3Dust-plumes power intercontinental microbial migrations 2Following Phragmites home 2
...
D.R. Harper and C. Grose (1994) • Includes: virus structure & replication, fundamental virology, immunology and pathogenesis, vaccines and antiviral drugs, novel diagnostic techniques, cloning a...
IHC Polymer Detection Kit (Rabbit DAB)150 slides...
...
Biology Products: