Navigation Links
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA


Introduction
Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification of recombinant histidine-tagged (His-tagged) proteins from a variety of expression systems.

The synthesis of IMAC resins begins with the derivatization of an appropriate wide-pore base resin with metal chelating groups, such as iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA). Specific transition metal ions usually Cu2+, Ni2+, Co2+, Ca2+, or Zn2+ are then immobilized to form a charged support. IMAC resins exhibit high affinity and metal-dependent selectivity for His-tagged proteins, as well as naturally occurring proteins that are rich in histidine, cysteine, aspartic acid, or glutamic acid residues.

To obtain highly purified proteins from an IMAC system, a series of method development experiments is typically required. These experiments involve optimization of the mobile phase pH, buffer system, flow rate, concentration of the competitive eluting agents, and a number of other potential parameters that may also be examined, depending on purity and recovery requirements. Usually, these would require a series of tedious and repetitive operations by the researcher, including the loading and recharging of the column with fresh metal ions after every run in order to ensure reproducible results.

Bio-Rad has developed an extremely rapid and efficient method using the BioLogic DuoFlow high-resolution chromatography system to purify a 75 kD His-tagged protein from crude E. coli lysate on a Profinity IMAC Ni2+-charged resin. In the present study, the concentration of a competitive eluting agent, imidazole, was optimized using the BioLogic DuoFlow system by executing multiple repetitive chromatography runs with a nine-buffer system. The plumbing setup of the system included an SV5-4 select valve (5-port, 4-position) and an AVR9-8 select valve (9-port, 8-position), which were both designed for flexible automated operation. This multiple inlet port configuration, coupled with the buffer scouting feature of the BioLogic DuoFlow software, allowed us to complete this experiment with a single linked scouting run and virtually no user intervention.


Methods
Setup
The column, UV detector, conductivity monitor, BioFrac fraction collector, SV5-4 select valve, AVR7-3 automated sample injection valve, and AVR9-8 select valve were connected to the workstation and controller as illustrated in Figure 1. All nine buffers, with the following compositions, were filtered and connected to the indicated SV5-4 and AVR9-8 valve ports:

A1: loading or equilibration buffer (50 mM potassium phosphate, 50 mM EDTA, 300 mM NaCl, pH 7.5)
A2: cleaning buffer (50 mM potassium acetate, 300 mM NaCl, pH 4.0)
A3: charging buffer (100 mM NiSO4, 300 mM NaCl, pH 4.3)
A4: loading buffer (50 mM potassium phosphate, 300 mM NaCl, pH 8.0)
B1: elution buffer 1 (loading buffer + 75 mM imidazole)
B2: elution buffer 2 (loading buffer + 100 mM imidazole)
B3: elution buffer 3 (loading buffer + 250 mM imidazole)
B6: elution buffer 4 (loading buffer + 50 0 mM imidazole)
B7: elution buffer 5 (loading buffer + 750 mM imidazole)

Experimental
A 1 ml Econo-Column chromatography column (0.5 cm ID x 5 cm) was packed manually with Profinity IMAC Ni2+-charged resin (60 m). Five separate runs were conducted, with either 75, 100, 250, 500, or 750 mM imidazole in the elution buffer. Following elution and a subsequent wash step, the column was regenerated using the loading and charging buffers listed under Setup. These five successive runs were linked through the buffer scouting function of the BioLogic DuoFlow software. For each run, the column was preequilibrated with 10 column volumes (CV) of loading buffer at a flow rate of 1.5 ml/min. Crude E. coli lysate (250 l) containing a 75 kD His-tagged protein was loaded into a 3 ml injection loop and injected into the column through an AVR7-3 valve. Host protein contaminants, which were not retained by the Profinity IMAC resin (Figure 2), were removed by washing the column with 10 CV of the loading buffer. The 75 kD His-tagged protein was then eluted with 5 CV of imidazole elution buffer, followed by an additional wash with 10 CV of the loading buffer to eliminate residual proteins from the column. The column was then regenerated as detailed in the following steps using 10 CV of each buffer: metal removal with the discharging buffer; washing with the cleaning buffer; recharging of the metal ion with the charging buffer; removal of excess metal ions with the cleaning buffer; and finally, reequilibration with the loading buffer.

In order to confirm the separation of the target protein from other E. coli proteins in the crude sample, the peak fractions we re analyzed by electrophoresis on a Criterion Tris-HCl 415% gradient polyacrylamide gel.


Results

The elution profile during each run was monitored at 280 nm, and a series of 1 ml fractions was collected using a BioFrac fraction collector.

The resulting chromatograms obtained with the 75, 100, 250, 500, and 750 mM imidazole elution buffers were superimposed using the Trace Compare feature provided within the BioLogic DuoFlow software; these are illustrated in Figure 3.

The data in Figures 2 and 3 indicate that optimal results were obtained with an elution buffer consisting of 250 mM imidazole, 50 mM potassium phosphate, 300 mM NaCl, pH 8.0. This elution buffer provided the most effective separation and removal of the impurities (Figure 2, lanes 5 and 6, respectively).


Conclusions
The development of rapid and efficient methods for the chromatographic isolation and characterization of biomolecules requires a chromatography system that is capable of advanced automated operation with minimal user intervention. This study demonstrates that the sophisticated valve configuration, high degree of flexibility, and versatile software of the BioLogic DuoFlow system make it ideal for meeting the demands of a modern method development laboratory.


Coomassie is a trademark of BASF Aktiengesellschaft.

Information in this tech note was current as of the date of writing (2004) and not neccessarily the date this version (Rev A, 2005) was published.


back to top
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
11. Rapidly Prepare Probes and Markers for Small RNA Analysis
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/27/2016)... ... ... Doctors in Italy, Japan, the UK and the US have reached some surprising ... its link to malignant mesothelioma. Surviving Mesothelioma has just posted the details of their ... , The studies analyzed for the new report included more than 3,447 cancer patients. ...
(Date:5/26/2016)... NEW YORK , May 26, 2016 ... announced today that it will be a featured presenter at ... 2016 in New York City at ... Denis Corin , Q BioMed Inc. CEO, is scheduled ... presentation will cover the company,s business strategy, recent developments and ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lajollacooks4u has become a rising hotspot ... rated one of its top attractions. Fortune 500 companies, such as Illumina, Hewlett-Packard, ... unique and intimate team-building experience. , Each event kicks off with an olive oil ...
(Date:5/25/2016)... , ... May 25, 2016 , ... Scientists at the ... options being tried for mesothelioma may be hampering the research that could lead to ... Click here to read it now. , The team evaluated 98 ...
Breaking Biology Technology:
(Date:3/14/2016)... , Allemagne, March 14, 2016 ... ) - --> - Renvoi : image ... --> --> ... biométriques, fournit de nouveaux lecteurs d,empreintes digitales pour ... de DERMALOG sera utilisé pour produire des cartes ...
(Date:3/10/2016)... 10, 2016   Unisys Corporation (NYSE: UIS ... (CBP) is testing its biometric identity solution at the ... to help identify certain non-U.S. citizens leaving the country. ... designed to help determine the efficiency and accuracy of using ... and will run until May 2016. --> ...
(Date:3/8/2016)... March 8, 2016   Valencell , the ... announced it has secured $11M in Series D ... a new venture fund being launched by UAE-based ... from existing investors TDF Ventures and WSJ Joshua ... continue its triple-digit growth and accelerate its pioneering ...
Breaking Biology News(10 mins):