Navigation Links
Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A

Chris Moran, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547


Introduction
Fast and efficient protein purification is essential to explore the structure and function of proteins in the human proteome. Successful protein purification strategies use experimental conditions where proteins of interest are stable and have chromatographic properties different from contaminating proteins. Significant time is often required to optimize chromatography parameters, such as buffer pH and gradient characteristics. Furthermore, purification strategies need to be easily scaled as the amount of protein required increases from milligram to gram quantities.

The new Maximizer buffer blending system, a component of the BioLogic DuoFlow chromatography system, significantly decreases the time and resources required for chromatography method development. With a single set of three salt solutions, the system can perform several chromatography experiments, varying the pH or gradient characteristics, or both, for each run. It is not necessary to prepare separate buffers for each chromatography experiment, and the system performs the experiments unattended. Data from the experiments are easily viewed and compared using the new Trace Compare feature of the BioLogic DuoFlow software.


Application
We were interested in purifying a recombinant protein in milligram quantities, anticipating future scale-up to preparative quantities. The 25 kD protein of interest is overexpressed as an E. coli inclusion body. Information from similar proteins suggested a stability range of pH 6.09.0. The protein was solubilized and
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
5. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
6. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
7. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
8. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
9. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
10. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
11. Rapidly Prepare Probes and Markers for Small RNA Analysis

Post Your Comments:
(Date:11/18/2009)...ational Institute of Standards and Technology (NIS...ve method for detecting and measuring elusive haza...ible spoilage in food or pesticides distributed in...re sensitive than conventional techniques for dete...ke water molecules, having distinct electrically p...
(Date:11/18/2009)... Imagine a polka-dotted postage stamp that can sni...changing colors. , As reported in the Sept. 13 i...slick and his team at the University of Illinois h...etection of toxic industrial chemicals (TICs) that...ualizing odors. This sensor array could be useful...
(Date:11/18/2009)..., Calif., Nov. 18 Sangam...ay that data from the University of Pennsylvania i...amo,s zinc finger nuclease (ZFN) based product, SB...urely disclosed on the internet. ,, Data were p...Pennsylvania School of Medicine from a single subj...
(Date:11/18/2009)...alif., Nov. 18 Masimo ...y(TM) and Measure-Through Motion and Low-Perfusion...-based Uni.H.A., one of the largest healthcare Gro...igned a multi-year purchasing agreement for "gold ... agreement offers preferred contract pricing to Un...
Breaking Biology Technology:Prototype NIST method detects and measures elusive hazards 2Opto-electronic nose sniffs out toxic gases 2Opto-electronic nose sniffs out toxic gases 3Sangamo BioSciences Provides Update on Phase 1 Safety Trial of SB-728-T for HIV/AIDS 2Uni.H.A. in France Signs Purchasing Agreement for Masimo SET(R) Pulse Oximetry Products 2Uni.H.A. in France Signs Purchasing Agreement for Masimo SET(R) Pulse Oximetry Products 3Uni.H.A. in France Signs Purchasing Agreement for Masimo SET(R) Pulse Oximetry Products 4
... many ways, Promega Corp . has given Northwester...ny. , ,Quiet to those outside the life-science rea...uburban Madison walls, it,s anything but silent, a... far, that innovation has produced about 200 paten...g 130 new product innovations since 2002. , ,That ...
...tructure has always been important to the economic...infrastructure has traditionally been viewed as tr... highways, water, and power (electricity). Today, ... network infrastructure and the ability to have br...as you would not locate your company on some back ...
...embly Speaker Mike Huebsch , R-West Salem, and St... eight legislators to serve on the Speaker,s Task ...ipartisan task force is charged with exploring a n... state government, and learning from successful pr...dition to Montgomery, who will serve as chair of t...
Other Biology Technology:Promega Corporation: Wisconsin's quiet innovator 2Promega Corporation: Wisconsin's quiet innovator 3Promega Corporation: Wisconsin's quiet innovator 4Promega Corporation: Wisconsin's quiet innovator 5States need to be more "connectivity conscious" 2States need to be more "connectivity conscious" 3States need to be more "connectivity conscious" 4Eight lawmakers named to Speaker's IT task force 2
(Date:11/20/2009)...With the introduction of a single bacterial gene i...chnology in the Netherlands achieved three improve...aste material: ,More ethanol, less acetate and eli...ek the invention was published in the scientific j..., Car fuel , Bioethanol is made by the yeast ...
(Date:11/20/2009)... This release is available in German . , In...cal Investigation , Dr. Stefanie Eyerich and Dr. K...Imperial College London and Istituto Dermopatico d...ng discovery. It represents a milestone on the way...ory skin diseases such as psoriasis and allergic r...
(Date:11/20/2009)...Chronic obstructive pulmonary disease (COPD), a re...itis or emphysema, is the fourth leading cause of ...D and while researchers have yet to find any real ...ch Institute at The University of Western Ontario ...ques to gain new insight into the disease. , ...
Breaking Biology News(10 mins):Delft breakthrough in bioethanol production from agricultural waste 2Discovery of new type of immune cells regulating inflammation in chronic diseases 2Gaining a better picture of lung disease 2Heat forms potentially harmful substance in high fructose corn syrup 10357 1As More Cyclists Hit the Road Serious Injuries Rise 59456 1As More Cyclists Hit the Road Serious Injuries Rise 59456 2Choyce Peterson Goes the Extra Mile for Visiting Nurses 59452 1Choyce Peterson Goes the Extra Mile for Visiting Nurses 59452 2
...n ocean-bottom rocks are more abundant and diverse...he planet,s oceanic crust, according to results of...nal Nature. , The findings pose intriguing quest... Earth and life. , Once considered a barren plain...cky regions appear to be teeming with microbial li...
...loor, microbial life is feeding on fresh volcanic ...ven the most optimistic scientists thought possibl...urnal Nature, scientists have found bacteria growi...usands- to ten-thousand times (three to four order...overlying waters. , Researchers from the Woods H...
...A naturally occurring molecule made by symbiotic g...inflammatory bowel disease, according to scientist...Most people tend to think of bacteria as insidious... Mazmanian, an assistant professor of biology at C...lationship between "good" bacteria and their mamma...
Other Biology News:Bacteria 'feed' on earth's ocean-bottom crust 2Scientists find bacteria thriving on a feast of seafloor rock 2Getting better with a little help from our 'micro' friends 2Getting better with a little help from our 'micro' friends 3
Hoagland's No. 2 Basal Salt Mixture from Sigma-Aldrich
Iscove's Modified Dulbecco's Medium (IMDM) from Sigma-Aldrich
...00 Q TRAP(TM) LC/MS/MS System, a high performance ...trometer for the drug discovery, drug development ...ap (LIT) Technology from the leaders in mass spect...e analysis with the proven, high sensitivity quant...
Dulbecco's Modified Eagle's Medium - high glucose (DME) from Sigma-Aldrich
Biology Products: