Navigation Links
Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

fferent regions of the same target transcript. The three GSP pairs generate 128 bp, 100 bp, and 99 bp products. Duplicate reactions using each primer pair with each reaction mix (A or iQ supermix) were run for each template. The performance of the iQ supermix reactions was quite consistent for each primer pair (Figure 4A), whereas the performance of the mix A-based reactions varied for each GSP (Figure 4B).

In the iQ supermix reactions, the average CT for all six traces representing the amplification of the macula sample with three different primer pairs was 19.9 0.3 cycles. The average CT for the periphery-derived sample was 21.4 0.2 cycles, resulting in an average of a 1.5 cycle difference between the two regions of human neural retina (Figure 4A). This differential expression profile for the macula-enriched gene transcript was the same as that obtained in the two previous experiments with iQ supermix reactions (Figures 2 and 3).

The traces representing the accumulation of PCR products in the mix A-based reactions varied with each GSP (Figure 4B), in contrast to the traces for the iQ supermix reactions (Figure 4A). In the mix A-based reactions, the 128 bp fragment was not amplified at all, whereas the products generated by the other two primer pairs were amplified at different rates. The amplification curves for the 100 bp fragment crossed threshold fluorescence at 25.4 cycles in the macula reactions and 25.8 in the periphery, while the curves for the 99 bp fragment from closer to the 3' end of the target mRNA crossed the threshold at 21.5 cycles in the macula reactions and 23.1 in the periphery. The average CT values for the two GSP pairs that resulted in the expecte
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
2. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
5. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
6. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
7. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
8. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
9. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
10. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
11. Real-Time PCR: General Considerations, Rev A
Post Your Comments:
(Date:9/2/2014)... September 02, 2014 Opertech Bio, ... highly efficient, cost effective approach to sensory evaluation, ... 8,820,265 by the U.S. Patent and Trademark Office, ... , “Opertech’s taste evaluation technology called ... automated high throughput system for rapid characterization of ...
(Date:9/2/2014)... Sept. 2, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or ... The orders consist of three large odour control systems for ... "These orders are consistent with the market resurgence ... Bruijns , President and CEO. "After a difficult period of ... now beginning to see sales growth. Our lower cost structure, ...
(Date:9/1/2014)... , Sept. 1, 2014 Reportlinker.com announces ... in its catalogue: India Solid Waste ... http://www.reportlinker.com/p02350445/India-Solid-Waste-Management-Vehicles-Market-Forecast-and-Opportunities-2019.html In ... the main governing bodies responsible for complete handling ... On an average, around 135,000 MT solid waste ...
(Date:8/31/2014)... 31, 2014 2014 Market ... a professional and in-depth research report on ... Polyacrylamide information, including Polyacrylamide definition, classification, application, ... industry overview. This research covers the international ... as global industry analysis covering macroeconomic environment ...
Breaking Biology Technology:Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 2India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 3India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 4India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 5India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 6India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 7India Solid Waste Management Vehicles Market Forecast and Opportunities, 2019 8International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 2International Polyacrylamide Industry & Copper Iodide Market Data Analysis Now Available at DeepResearchReports.com 3
... with Third FDA Market Clearance Since ... ... Corp.,(Nasdaq: ESMC ) today announced that it has received 510(k) ... VascuView(TM),Visual Ultrasound System for use with assisted vascular access. Its,subsidiary, Escalon ...
... with the, Nikon Ti Inverted Microscope System to Provide Advanced ... ... Performance, MELVILLE, N.Y., Jan. 29 Nikon Instruments, Inc., ... scanning systems, which seamlessly integrates with the new Ti-E,research inverted ...
... Pre-Clinical Studies to Commence for Neurological Diseases ... Cryo-Cell International,Inc. (OTC Bulletin Board: CCEL) ("Cryo-Cell" ... most established family cord blood banks, today ... development agreement with Saneron CCEL,Therapeutics, Inc. ("Saneron") ...
Cached Biology Technology:Escalon(R) Announces FDA 510(K) Clearance for VascuView(TM) Visual Ultrasound System For Assisted Vascular Access 2Escalon(R) Announces FDA 510(K) Clearance for VascuView(TM) Visual Ultrasound System For Assisted Vascular Access 3Escalon(R) Announces FDA 510(K) Clearance for VascuView(TM) Visual Ultrasound System For Assisted Vascular Access 4Nikon Instruments Launches Fully-Automated A1 Confocal Laser Microscope Series 2Nikon Instruments Launches Fully-Automated A1 Confocal Laser Microscope Series 3Nikon Instruments Launches Fully-Automated A1 Confocal Laser Microscope Series 4Cryo-Cell Announces Research Collaboration Agreement with Saneron CCEL Therapeutics, Inc. 2Cryo-Cell Announces Research Collaboration Agreement with Saneron CCEL Therapeutics, Inc. 3Cryo-Cell Announces Research Collaboration Agreement with Saneron CCEL Therapeutics, Inc. 4
(Date:9/1/2014)... of research shows an association between TV viewing and ... a new Cornell University study points out that not ... people to eat twice as much as other programs! ... while snacking your mouth will see more action too!" ... article just published in the Journal of the ...
(Date:9/1/2014)... that corals whose calcium-carbonate skeletons form the ... that rely entirely on ocean currents to deliver ... now scientists at MIT and the Weizmann Institute ... they are far from passive, engineering their environment ... enhance their ability to exchange nutrients and dissolved ...
(Date:9/1/2014)... the alteration of a single nucleotidethe basic building block of DNAcould ... disability. The study appears in The Journal of Cell ... by a defect in a gene on the X chromosome called ... 230 women and 1 in 360 men carry a so-called premutation, ... the FMR1 gene is slightly longer than normal. These ...
Breaking Biology News(10 mins):Can action movies make you fat? 2Nature's tiny engineers 2Nature's tiny engineers 3A nucleotide change could initiate fragile X syndrome 2
... becomes, common ailments like fungal infections can outrun the ... immune systems (like premature babies, AIDS victims or those ... contracting a fungal infection can be deadly. Now ... sea to collect unique chemicals drugs of the ...
... The National Space Biomedical Research Institute (NSBRI) ... Medical Center as one of four young investigators ... Program. Trani is a postdoctoral fellow in ... Department of Biochemistry and Molecular & Cellular Biology. ...
... genotyping technologies offer new opportunities to explore how ... the challenge of analyzing huge amounts of genetic ... at the Lausanne Branch of the international Ludwig ... of Bioinformatics (SIB) and the University Hospital of ...
Cached Biology News:An underwater drugstore? 2GUMC young scientist selected postdoc Fellow at National Space Biomedical Research Institute 2New tool for genome-wide association studies 2
... Vector is a derivative of the pAcHLT-A Vector ... fluorescent protein (BFP), followed by a 6xHis tag ... expressed as BFP-6xHis fusion proteins when cloned into ... Stu I, Sac I, Not I, Pst I, ...
Mouse monoclonal antibody to ALAS2 - aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia)...
Mouse polyclonal antibody to GRID2 - glutamate receptor, ionotropic, delta 2...
Mouse polyclonal antibody raised against a partial recombinant WRB. NCBI Entrez Gene ID = 7485...
Biology Products: