Navigation Links
Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

f iQ supermix rescued the assay, resulting in accumulation of the macula-derived 128 bp products crossing the threshold of 100 RFU after 19.7 cycles (Figure 2) almost 2 cycles earlier than in the mix A-based reactions with macula cDNAs for template (Figure 1). In the iQ supermix reactions containing periphery-derived cDNAs, the 128 bp product crossed this threshold value at 21.2 cycles (Figure 2), 1.5 cycles later than the macula reactions with the iQ supermix and almost 2 cycles earlier than with the mix A periphery reactions in experiment 1 (Figure 1). Equivalent mix A reactions run at the same time failed to amplify (Figure 2). As shown in the following experiment, the reproducibility of these results as well as the stability of the iQ supermix reagents held up over time.

Experiment 3: Performance of iQ Supermix Reactions Over Time
Real-time RT-PCR was performed as described for experiment 2, except that the iQ supermix (2x) stock used in these reactions had been stored for 4 months at 20C. The 128 bp segment of the macula-enriched transcript was again successfully amplified. The amplification curve for the macula sample crossed the threshold of 100 RFU after 20.1 cycles, while the periphery sample crossed the threshold 1.7 cycles later at 21.8 (Figure 3). The iQ supermix is therefore more stable over time than mix A.

Experiment 4: Amplification of a Specific Transcript Using Three Different Pairs of GSPs With iQ Supermix vs. Mix A
In order to test whether primer design can affect the reproducibility of amplification curves obtained for a specific transcript in a specific tissue, real-time RT-PCR was performed using three pairs of primers designed to amplify di
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
2. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
5. Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
6. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
7. Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
8. Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A
9. Real-Time Immuno-PCR on the iCycler iQ System, Rev A
10. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
11. Real-Time PCR: General Considerations, Rev A
Post Your Comments:
(Date:12/24/2014)... December 23, 2014 “Preparative & ... HPLC, Flash), Consumables (Columns, Solvents, Buffers, Valves, Guages, ... & Agriculture) - Forecasts to 2019” provides a ... opportunities, current market trends, and strategies impacting the ... estimates and forecasts of the revenue and share ...
(Date:12/24/2014)... York (PRWEB) December 23, 2014 According to ... Long Island Affiliate of ITRA Global, the national office market ... steady recovery. This is evidenced by the third quarter’s ... stock market. Low energy costs have held inflation down ... only negative is the housing market remaining soft. , This ...
(Date:12/24/2014)... BEIJING , Dec. 23, 2014 ... or the "Company"), a leading fully integrated plasma-based ... today announced that its majority-owned subsidiary, Shandong Taibang ... Practices ("GMP") certification from the China Food and ... factor production facility. As previously disclosed in the ...
(Date:12/24/2014)... NEW YORK , Dec. 23, 2014  NeuroLifeSciences ... of mazindol in children with attention deficit/hyperactivity disorder published ... 2014 . The paper, titled "Pilot ... deficit/hyperactivity disorder" ( Konofal et al, Drug ... 2014 ) shows that mazindol might be an ...
Breaking Biology Technology:Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 2Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 3
... Milwaukee-based provider of financial technology solutions selected Opsware ... operations Tuesday. Metavante plans to use Opsware to automate ... center information technology environment. , ,We selected Opsware ... complexity and cost in the data center, said Brian ...
... and be more responsive to the needs of their ... they provide as a result of two new products ... company. API Software announced Tuesday the availability of the ... scheduling application, which recently acquired two new contracts. ...
... Third Wave Technologies Inc. , a Madison-based provider of ... 2003 last Tuesday. The year was transitional for Third ... financial resources away from the genomic tools market and ... revenue of $36.3 million and a net loss of ...
Cached Biology Technology:API Releases New Software, Acquires Additional Contracts 2
(Date:12/22/2014)... Dec. 22, 2014 Research and Markets ... the addition of the "The Global ... their offering. ... an overview of the global digital media ... control and monitor piracy by securely and ...
(Date:12/19/2014)... Research and Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ... "iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & ... offering. http://photos.prnewswire.com/prnh/20130307/600769 ... July 2012, Apple introduced the fingerprint reading feature ... only device of Apple incorporating such a feature, ...
(Date:12/19/2014)... Dec. 18, 2014  23andMe, Inc., the leading personal ... that pinpoints fine-scale differences in genetic ancestry of individuals ... Since immigrants first arrived more than four ... has served as a meeting place for peoples ... and the ongoing mixing of peoples with African, European, ...
Breaking Biology News(10 mins):The Global Watermarking and Fingerprinting Markets 2iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 223andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4
... Ann Liebert, Inc. is pleased to announce significant growth ... Impact Factors published by Thomson Reuters. More than half ... the majority achieving an increase of 10% and higher. ... editorial standards for exceptional quality, supported by outstanding review ...
... Smithsonian Conservation Biology Institute and George Mason University ... complex that embodies the concept of the living ... Studies Program has trained future conservationists with an ... Smithsonian National Zoological Park,s 3,200-acre facility in Front ...
... YORK, N.Y. (June 30, 2011) Autism Speaks Weatherstone Pre-Doctoral ... a 2010 fellow, recently published new findings in the journal ... autism. Their research is based on a mouse model that ... to be associated with autism spectrum disorder (ASD), known as ...
Cached Biology News:Smithsonian Conservation Biology Institute and George Mason University expand partnership 2Weatherstone Fellows publish research as Autism Speaks announces 2011 Weatherstone Fellows 2Weatherstone Fellows publish research as Autism Speaks announces 2011 Weatherstone Fellows 3
... Neogens enzyme-linked immunosorbent assay (ELISA) test ... screening devices for the detection of drugs ... kits are intended for forensic use only. ... be confirmed by a quantitative method such ...
Cag-A (IgG) -Ab EIA Sample Size: 10 l...
... pending) is a protein based inhibitor of ... the most common and troublesome RNases including ... SUPERaseIn can be used in any application ... is ideal for in vitro transcription and ...
... Inhibitor is a recombinant human protein produced in ... neutral pancreatic RNase A type enzymes. The ... binds RNases in a 1:1 ratio. The enzyme ... many tissues and cell types. Addition of the ...
Biology Products: