Navigation Links
Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys

lly to a BioFlo 6000 Mobile Pilot Plant fermentor equipped with an ML-6100 touch-screen controller and 100 L working volurne vessel, (all equipment manufactured by New Brunswick Scientific, Edison, NJ). NBS' proprietary AFS-BioCommand software was used to control a multi-loop cascade strategy, wherein each of four process parameters were altered in sequence so as to maintain the desired D.O. level. Capable of managing up to eight fermentors, bioreactors, or ancillary instruments, this software automates the tedious and error-prone jobs of logging data, computing real and derived process values, and altering setpoints on the basis of time or metabolic events. In this study, AFS-BioCommand was programmed to maintain a D.O. level of 30% of air saturation by automatically increasing agitation rates from 200 to 450 rpm, then vessel pressure from 0.35 to 0.75 bar, aeration rate from 0.5 to 1 VVM and simultaneously shifting temperature from 28C to 25C, when D.O. fell below its setpoint. Based on optimization studies conducted on the bench level, pH was maintained between 6.2 and 6.8. A deadband was easily configured by using the local controller for the low setpoint and the supervisory control software for the high setpoint.


Discussion
Utilizing the multi-loop cascade strategy provided several benefits. First and foremost, D.O. was able to be maintained at optimal rates for production of rapamycin, a feat unable to be accomplished using a single loop control strategy ( see figure ). Agitation rates could also be significantly reduced, to minimize shear forces and enable higher yields of both cells and secondary metabolites.

Temperature was also lowered from that optimal for culture growth to the optimal range for rapamycin production. The lower temperature had the side benefit of slowing cell growth, thereby decreasing oxygen demands, which h
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
3. Optimized Dengue RT-PCR
4. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
5. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
6. An Easy-to-Use Research Fermentor/Bioreactor
7. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:7/10/2014)... PETERSBURG, Russia , July 10, 2014 ... Russian Federation Dmitry Medvedev , presented ... biopharmaceutical company BIOCAD . The Company is developing ... scope of MabNext project BIOCAD develops a number of ... cancer and autoimmune diseases. The ceremony took place at ...
(Date:7/10/2014)... PROVO, Utah , July 10, 2014 ... genome annotation and interpretation, today announced an ... sequencing (NGS) analytics for Lineagen,s NextStep Dx ... evaluation of neurodevelopmental and neurological disorders, currently ... chromosomal microarray (CMA) testing service used by ...
(Date:7/10/2014)... 2014   Ceres, Inc . (Nasdaq: ... today financial results for the three months ended May ... Ceres reported that the company and its ... growing season in Brazil, which concluded in June, despite ... evaluation areas for part of the growing season. Yields ...
(Date:7/10/2014)... may help in the fight against terrorism with the ... of explosives with the use of light and special ... and Actuators B: Chemical , the researchers describe a ... concentrations as low as 6.3 ppm (parts per million). ... minutes. , "Traditionally explosives detection has involved looking for ...
Breaking Biology Technology:Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Detecting trace amounts of explosives with light 2
... Diagnostics, a leading developer of biofluid-based molecular diagnostic ... the appointment of David Okrongly, Ph.D., as chief ... medical officer, and Sally Bowden, Ph.D., as director ... of cardiothoracic surgery Harvard University and Michael Manyak, ...
... Glioblastoma is one of the most aggressive forms ... tumor, glioblastoma will often infiltrate the surrounding brain tissue, ... chemotherapy or radiation. Likewise, several mouse models of glioblastoma ... a new study, a team led by scientists at ...
... announced today the launch of Curve™ Image Guided Surgery. ... surgery, is unveiling at the Congress of Neurological Surgeons ... of enhancements based on years of feedback from our ... for Brainlab and has helped to produce the ultimate ...
Cached Biology Technology:Exosome Diagnostics Announces Additions to Management and Scientific Advisory Board as Company Expands Clinical Validation Studies in Oncology 2Exosome Diagnostics Announces Additions to Management and Scientific Advisory Board as Company Expands Clinical Validation Studies in Oncology 3Exosome Diagnostics Announces Additions to Management and Scientific Advisory Board as Company Expands Clinical Validation Studies in Oncology 4Exosome Diagnostics Announces Additions to Management and Scientific Advisory Board as Company Expands Clinical Validation Studies in Oncology 5Nanoparticles seek and destroy glioblastoma in mice 2Nanoparticles seek and destroy glioblastoma in mice 3Brainlab Launches Newest Innovation in Image Guided Surgery Technology 2Brainlab Launches Newest Innovation in Image Guided Surgery Technology 3
(Date:7/13/2014)... more effective treatments for cachexia, a profound wasting of ... cancer patients, raising their risk of death, according to ... been tried to reverse the condition, which may cause ... but none have had great success. , Scientists reporting ... Nature , led by Bruce Spiegelman, PhD, demonstrated that ...
(Date:7/11/2014)... the City of Entiat on Tuesday, July 8, 2014. ... grew to over 1,000 acres by evening. Three outbuildings ... investigation by the Washington Department of Natural Resources. The ... in developing fire control strategies and tactics. Other cooperators ... of Natural Resources, (WA DNR), Bureau of Land Management, ...
(Date:7/11/2014)... scientists have set a "mouse TRAP" to capture the ... recent study published in the Journal of Clinical ... technique called TRAP to extract cellular and genetic information ... scientists at the Rockefeller Institute for Medical Research in ... protein-making machinery, or ribosomes, of the cell type of ...
Breaking Biology News(10 mins):Antibody halts cancer-related wasting condition 2Antibody halts cancer-related wasting condition 3
... River in Washington, D.C. is showing multiple benefits from ... improved water clarity have increased the abundance and diversity ... to direct measurements taken during the 18-year field study. ... the lower Potomac has doubled, the area covered by ...
... YORK, Sept. 7 Reportlinker.com announces that a new ... Biomarkers - technologies,markets and companies ... This report follows the broad definition of ... measured and evaluated as an indicator of normal biological ...
... 2009, newly installed photovoltaic (PV) cells world-wide produced a peak ... 5.8 GW was located in Europe. Similarly to previous years, ... of the world,s new solar systems were installed. By the ... (existing and newly installed) was 16 GW, which is about ...
Cached Biology News:Potomac River: 10-fold increase in native submerged vegetation reflects improved water quality 2Reportlinker Adds Biomarkers - technologies, markets and companies 2Reportlinker Adds Biomarkers - technologies, markets and companies 3Reportlinker Adds Biomarkers - technologies, markets and companies 4Reportlinker Adds Biomarkers - technologies, markets and companies 5Reportlinker Adds Biomarkers - technologies, markets and companies 6Reportlinker Adds Biomarkers - technologies, markets and companies 7Reportlinker Adds Biomarkers - technologies, markets and companies 8Reportlinker Adds Biomarkers - technologies, markets and companies 9Reportlinker Adds Biomarkers - technologies, markets and companies 10Reportlinker Adds Biomarkers - technologies, markets and companies 11Reportlinker Adds Biomarkers - technologies, markets and companies 12Reportlinker Adds Biomarkers - technologies, markets and companies 13Reportlinker Adds Biomarkers - technologies, markets and companies 14Reportlinker Adds Biomarkers - technologies, markets and companies 15Reportlinker Adds Biomarkers - technologies, markets and companies 16Reportlinker Adds Biomarkers - technologies, markets and companies 17Reportlinker Adds Biomarkers - technologies, markets and companies 18Reportlinker Adds Biomarkers - technologies, markets and companies 19Reportlinker Adds Biomarkers - technologies, markets and companies 20Three-quarters of new solar systems worldwide were installed in the EU in 2009 2Three-quarters of new solar systems worldwide were installed in the EU in 2009 3
BI-1 Polyclonal Antibody Storage Temperature: -20C Shipping: gel pack Shelf-Life: 12 months...
... cells of embryonal carcinoma of testis and ovary ... like seminoma. Glandular epithelium of yolk sac tumor ... cells of stomach and acini cells of pancreas ... Epitope: Carbohydrate portion on human embryonal carcinoma ...
... Endothelin-1 (human, porcine) Endogenous potent ... ETA receptor and modulates vascular tonus. ET-1, ... three distinct endothelin genes. ET-2 and ET-3 ... two and six amino acids, respectively. All ...
HP 1 alpha Immunogen: Synthetic peptide between amino acids 1-100 of the human HP 1 alpha protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products: