Navigation Links
Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys

lly to a BioFlo 6000 Mobile Pilot Plant fermentor equipped with an ML-6100 touch-screen controller and 100 L working volurne vessel, (all equipment manufactured by New Brunswick Scientific, Edison, NJ). NBS' proprietary AFS-BioCommand software was used to control a multi-loop cascade strategy, wherein each of four process parameters were altered in sequence so as to maintain the desired D.O. level. Capable of managing up to eight fermentors, bioreactors, or ancillary instruments, this software automates the tedious and error-prone jobs of logging data, computing real and derived process values, and altering setpoints on the basis of time or metabolic events. In this study, AFS-BioCommand was programmed to maintain a D.O. level of 30% of air saturation by automatically increasing agitation rates from 200 to 450 rpm, then vessel pressure from 0.35 to 0.75 bar, aeration rate from 0.5 to 1 VVM and simultaneously shifting temperature from 28C to 25C, when D.O. fell below its setpoint. Based on optimization studies conducted on the bench level, pH was maintained between 6.2 and 6.8. A deadband was easily configured by using the local controller for the low setpoint and the supervisory control software for the high setpoint.


Discussion
Utilizing the multi-loop cascade strategy provided several benefits. First and foremost, D.O. was able to be maintained at optimal rates for production of rapamycin, a feat unable to be accomplished using a single loop control strategy ( see figure ). Agitation rates could also be significantly reduced, to minimize shear forces and enable higher yields of both cells and secondary metabolites.

Temperature was also lowered from that optimal for culture growth to the optimal range for rapamycin production. The lower temperature had the side benefit of slowing cell growth, thereby decreasing oxygen demands, which h
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
3. Optimized Dengue RT-PCR
4. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
5. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
6. An Easy-to-Use Research Fermentor/Bioreactor
7. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
8. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
9. Oil-Free PCR Using the Hot Top Assembly
10. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:5/22/2015)... DALLAS , May 22, 2015 Baylor ... and the Translational Genomics Research Institute (TGen) in ... that will focus on accelerating early detection and treatments for ... science of both institutions will lead to new clinical trials ... therapies – bridging the gap from bench to bedside across ...
(Date:5/21/2015)... MO (PRWEB) May 21, 2015 Seventh ... the safety and efficacy of pharmaceutical products and medical ... Maryland Heights, MO 63043, a 50,000 sq. ft. building ... location, to enable strategic growth. Facility renovations will begin ... the new space will occur in September. , ...
(Date:5/21/2015)...  Susan Hedstrom, Executive Director of the Foundation ... to supporting research to advance the understanding and ... FPWR has received the organization,s largest donation to ... $1 million in support of the organization,s research ... "Our gift signifies our tremendous trust ...
(Date:5/21/2015)... San Francisco, CA (PRWEB) May 21, 2015 ... microbiome sequencing, has announced a partnership with PicnicHealth, ... records for patients. Participants diagnosed with Inflammatory Bowel ... as well as a complementary uBiome research kit. ... Summer 2014. , For more ...
Breaking Biology Technology:Precision Medicine Collaboration to Increase Options for Cancer Patients 2Precision Medicine Collaboration to Increase Options for Cancer Patients 3Precision Medicine Collaboration to Increase Options for Cancer Patients 4Seventh Wave Laboratories Purchases Building for Expansion, Upcoming Move 2Seventh Wave Laboratories Purchases Building for Expansion, Upcoming Move 3The Foundation for Prader-Willi Research Receives $1M Contribution in Support of Clinical Initiatives 2uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 3
... Nov. 20 CryoLife, Inc. (NYSE: CRY ),a ... it is scheduled to participate in the 19th Annual ... New York City on Tuesday,November 27, 2007. Steven ... deliver,the Company,s presentation beginning at approximately 1:30 p.m. EST. ...
... JOSEPH, Mich., Nov. 20 GeneGo, Inc., ... in systems biology, announced,today that Utrecht University ... program for drug innovation. MetaDrug is designed ... and off-target effects for,new compounds structures. Its ...
... Cepheid (Nasdaq:,CPHD) will present at the Piper Jaffray ... ET at The Pierre Hotel in New York. John ... Vice President,Finance and Chief Financial Officer, will provide a ... slides, may also be,accessed on Cepheid,s Web site at ...
Cached Biology Technology:CryoLife to Present at 19th Annual Piper Jaffray Health Care Conference in New York 2Utrecht University Licenses MetaDrug From GeneGo for Drug Innovation 2Cepheid to Present at the Piper Jaffray 19th Annual Healthcare Conference 2
(Date:5/21/2015)... May 21, 2015 The Sync Project™ ... music for health, today announced a collaborative partnership with ... partnership will center on collaboration on original research, joint ... step in the collaboration, The Sync Project and BerkleeICE ... as Medicine" in the 2015-2016 academic year.  ...
(Date:5/20/2015)... 20, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... owned subsidiary, 3D-ID LLC, a company engaged in biometric ... the biometrics technology portion of the recently awarded TIES ... Department of the Army. 3D-ID LLC has ... government, law enforcement and security agencies. ...
(Date:5/19/2015)...  Technology is rapidly advancing, outpacing enterprises, ability to ... Passwords and their management are soon to be rendered ... such as those developed by the FIDO Alliance™. With ... for BYOD, COPE, IoT, and mobile payments, the interconnected ... In response to the call for seamless, ...
Breaking Biology News(10 mins):The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 2The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 3NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 2NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 3HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3
... afternoon and you have no appetite and don,t want to do ... researchers from the ARC Centre of Excellence for Coral Reef Studies ... some large reef fish feel the same way. Researcher Dr ... activities necessary for survival, including hunting for food and finding mates. ...
... have shown that Fructus corni has a protective ... and antiseptic properties. 5-hydroxymethylfurfural, a compound extracted from ... hippocampal neurons damaged by H2O2. Prof. Mingyan Wang ... for the first time, used cytobiological and molecular ...
... have been awarded the distinction of Fellow by the American ... to this rank because of their efforts toward advancing science ... of all 388 AAAS fellows for 2013 are announced in ... four Smithsonian honorees are: Helen James, research ...
Cached Biology News:Reef fish find it's too hot to swim 2Smithsonian scientists honored as AAAS Fellows 2
p-Bcl-2 (Thr 74)...
Trx-2 (71G4)...
... polyclonal to Endothelin B Receptor Like ... Receptor-Like Protein 2 (ETBR-LP-2) has been ... been isolated from human brain and ... Synthetic peptide (unfortunately, the amino acid ...
...
Biology Products: