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RT-PCR Primers for the Study of Apoptosis

(lane 4). This may be a spliced version of Fas antigen found in testes or an artifact. Again, the amplification of the rat Fas antigen gene is specific. No band appeared on the control lanes with mouse and human cDNA libraries.

Figure 5

Conclusions

Apoptosis is one of the most actively studied areas in the biological sciences. Stratagenes RT-PCR primer sets for studying apoptosis are specific for genes involved in inducing and regulating the apoptotic response. These primer sets target human, rat, and mouse genes that encode proteins from the Fas and Fas ligand, Bcl-2, and ICE families. The specificity of these primers has been tested and confirmed in a variety of RT-PCR reactions. The availability of these primer sets will enhance research efforts in disciplines such as developmental biology, immunology, and tumor biology.

Acknowledgments

We wish to kindly thank Mark Dycaico for providing human genomic DNA

REFERENCES

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  2. Yonehara, S., Ishii, A., and Yonehara, M. (1989) J. Exp. Med. 169: 17471.

  3. Nagata, S., et al. (1994) Adv. Immunol. 57: 129.

  4. Douglas, P., et al. (1992) Science 256. 97.


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