Navigation Links
RT-PCR Primers for the Study of Apoptosis

a specific band at the expected length: For ICE- b, a 600-bp band was observed; for CPP32, a 540-bp fragment was observed and for ICE-Lap-3, a 600-bp band was observed. The genomic DNA and mouse and rat cDNA did not generate similar PCR products. The relatively weak bands seen for CPP32 may reflect the abundance of the CPP32 cDNA present in the cDNA library. Increasing the concentration of template in the reaction by 50% did not result in a significant increase of PCR products.

Figure 3

The ICE-Lap-3 and Fas antigen primer sets did not amplify the expected PCR product from the human muscle cDNA library. Repeated PCR attempts generated the same results. This may be explained by the lack of specific cDNA target in the library. A control primer, human b-actin, was used to test the cDNA library. An expected band was amplified for all human cDNA libraries tested (data not shown).

Mouse Fas Antigen and Fas Ligand

Several different mouse cDNA library templates were used to amplify Fas antigen and Fas ligand gene products (Figure 4). Both mouse Fas antigen and mouse Fas ligand RT-PCR primer sets amplified PCR products of the expected sizes. The Fas ligand gene has a wide distribution range in different tissues; however, the Fas antigen gene is differentially expressed in certain tissues. No amplification occurred in the control rat and human cDNA libraries.

Figure 4

Rat Fas Antigen

The rat Fas antigen gene was amplified in rat brain, lung, and heart cDNA libraries (Figure 5). The rat heart cDNA library generated a PCR product larger in size than expected


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
TAG: PCR Primers for the Study Apoptosis

(Date:7/22/2014)... July 22, 2014 The Board of Directors of ... has declared a quarterly dividend of 54.5 cents per common ... on September 9, 2014. The indicated annual dividend rate is ... BD is a leading medical technology company that partners with ... pressing and evolving health needs. Our innovative solutions are focused ...
(Date:7/22/2014)... of Standards and Technology (NIST) and California Institute ... for an atomic clock that is based on ... The microcomb clock, featured on the cover of ... Optica ,* is the first demonstration of all-optical ... of optical frequencies to lower microwave frequencies. (Optical ...
(Date:7/22/2014)... a senior lecturer in mechanical engineering at the University ... the Year by the North Texas section of the ... more than 130,000 members in 158 countries who develop ... assist the engineering community, benefiting the world. , ASME ... of Professional Engineers honors banquet. Baughn retired as an ...
(Date:7/22/2014)... 2014 Nuclear reactors are used to ... controlled fission chain reaction in nuclear reactors is used ... to drive a power- generating turbine. Currently nuclear reactors ... total electricity production. Nuclear reactor market is expected to ... and proposed nuclear reactor projects. , Early buyers will ...
Breaking Biology Technology:'Comb on a chip' powers new NIST/Caltech atomic clock design 2UT Dallas professor receives Engineer of the Year award 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 2Nuclear Energy Market: Global Industry Analysis, Size, Share, Analysis, Growth and Forecast 2013 - 2019 3
... YORK, Dec. 10, 2010 Ramius V&O Acquisition LLC, ... that it has sent a letter to the Board ... CYPB ) outlining its willingness to acquire all of ... $5.50 per share.  The offer is conditioned upon Cypress ...
... will help UK athletes reach the top of their ... the UK,s high performance sports agency, has reached an ... edge technology developed by Argento Diagnostics to improve training ... to monitor various proteins which reveal details about the ...
... ALBUQUERQUE, N.M. A benchtop version of the world,s ... seven-thousandth the thickness of a human hair has been ... researcher Jianyu Huang. To better study the anode,s ... a transmission electron microscope (TEM) at the Center for ...
Cached Biology Technology:Ramius Offers to Enter Into Immediate and Exclusive Negotiations to Acquire Cypress Bioscience for $5.50 Per Share 2Ramius Offers to Enter Into Immediate and Exclusive Negotiations to Acquire Cypress Bioscience for $5.50 Per Share 3Ramius Offers to Enter Into Immediate and Exclusive Negotiations to Acquire Cypress Bioscience for $5.50 Per Share 4Ramius Offers to Enter Into Immediate and Exclusive Negotiations to Acquire Cypress Bioscience for $5.50 Per Share 5Ramius Offers to Enter Into Immediate and Exclusive Negotiations to Acquire Cypress Bioscience for $5.50 Per Share 6Medical science helps UK athletes reach peak performance 2Medical science helps UK athletes reach peak performance 3World's smallest battery created at CINT nanotechnology center 2World's smallest battery created at CINT nanotechnology center 3
(Date:7/23/2014)... fly Chrysomya megacephala is commonly found in ... determine the time of death, referred to as the ... of C. megacephala from Tamil Nadu is ... and molecular characterization through generation of DNA barcoding. This ... open access Biodiversity Data Journal . , ...
(Date:7/23/2014)... and national governments struggle to deal with ever-growing piles ... the picture of just how much there is and ... journal Environmental Science & Technology , their study ... countries discard floods into just seven developing countries ... live there. , Knut Breivik and colleagues note that ...
(Date:7/23/2014)... SUP05 will play an increasingly important role in carbon ... minimum zones expand, according to research published this week ... Sciences . , University of British Columbia researchers plumbed ... Inlet, to chart how microbial community metabolism changes as ... very detailed picture of how SUP05 a bacterial ...
Breaking Biology News(10 mins):Dead body feeding larvae useful in forensic investigations 2Research charts the ecological impact of microbial respiration in the oxygen-starved ocean 2
... in Ireland are mislabelled, as compared to 7% in the ... Fish and Fisheries . This is the first ... sold in Ireland and the UK. Both countries operate under ... Using a DNA barcoding technique (COI barcoding gene), scientists ...
... of the treats of summerfresh, antioxidant-rich blueberrieshas new competition for ... contenders are keeping the title in the family. , Researchers ... the tropical regions of Central and South Americathe New World ... the blueberries sold in U.S. markets. , This finding is ...
... patient has been enrolled by researchers at The University of ... first double-blind clinical trial studying the safety and efficacy of ... to 19 days after an ischemic stroke. The Phase ... regenerative therapy developed by Aldagen that uses a patient,s own ...
Cached Biology News:Cod mislabeling 4 times more prevalent in Ireland than UK, study shows 2Summer's superfruit challenged: Latin American blueberries found to be 'extreme superfruits' 2Study uses new stem cell therapy in patients up to 19 days after stroke 2