Navigation Links
RT-PCR Primers for the Study of Apoptosis

a specific band at the expected length: For ICE- b, a 600-bp band was observed; for CPP32, a 540-bp fragment was observed and for ICE-Lap-3, a 600-bp band was observed. The genomic DNA and mouse and rat cDNA did not generate similar PCR products. The relatively weak bands seen for CPP32 may reflect the abundance of the CPP32 cDNA present in the cDNA library. Increasing the concentration of template in the reaction by 50% did not result in a significant increase of PCR products.

Figure 3

The ICE-Lap-3 and Fas antigen primer sets did not amplify the expected PCR product from the human muscle cDNA library. Repeated PCR attempts generated the same results. This may be explained by the lack of specific cDNA target in the library. A control primer, human b-actin, was used to test the cDNA library. An expected band was amplified for all human cDNA libraries tested (data not shown).

Mouse Fas Antigen and Fas Ligand

Several different mouse cDNA library templates were used to amplify Fas antigen and Fas ligand gene products (Figure 4). Both mouse Fas antigen and mouse Fas ligand RT-PCR primer sets amplified PCR products of the expected sizes. The Fas ligand gene has a wide distribution range in different tissues; however, the Fas antigen gene is differentially expressed in certain tissues. No amplification occurred in the control rat and human cDNA libraries.

Figure 4

Rat Fas Antigen

The rat Fas antigen gene was amplified in rat brain, lung, and heart cDNA libraries (Figure 5). The rat heart cDNA library generated a PCR product larger in size than expected
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: PCR Primers for the Study Apoptosis

(Date:5/17/2013)... Topricin Pain Relief and Healing Cream, ... among nationally recommended over-the-counter (OTC) health products 2012 ... News" Health in collaboration with "Pharmacy Times." ... Pharmacists Recommended designation” in the “Topical Analgesics” category, ... is the only homeopathic formula to earn that ...
(Date:5/17/2013)... 17, 2013 Dr. Sparano is Professor ... Health at the Albert Einstein College of Medicine and ... Montefiore Medical Center. He is also Associate Director ... leads the Einstein Breast Cancer Working Group, a multidisciplinary ... cancer research. He also serves as Vice Chair of ...
(Date:5/17/2013)... (PRWEB) May 17, 2013 Oven Industries ... capabilities. The 5R6-900 temperature controller has many user-friendly benefits. ... plugged into the wall as a self-contained temperature ... This distinctive detail makes the device unique, as well ... controller can also be used universally, which allows the ...
(Date:5/17/2013)... recipient,s immune system identifies the transplanted organ as foreign ... T cells, the immune cells that mediate rejection, must ... order to migrate to the transplanted organ. In this ... Fadi Lakkis and colleagues at the University of Pittsburgh ... cells is not required for migration. Instead, these cells ...
Breaking Biology Technology:Pharmacists' Picks: Topical BioMedics' Topricin Listed on Top Recommended OTC Health Products 2Joseph A. Sparano, MD, Named Vice Chair of ECOG-ACRIN Cancer Research Group 2Joseph A. Sparano, MD, Named Vice Chair of ECOG-ACRIN Cancer Research Group 3New Temperature Control Products Announced by Oven Industries Inc. 2
... Alexion,Pharmaceuticals, Inc. (Nasdaq: ALXN ) today announced ... Officer of Alexion,Pharmaceuticals, will present at the William ... 9:10 a.m., local time (10:10 a.m., eastern) on,Thursday, ... Mr. Sinha,s remarks will be available live. You ...
... Beckman,Coulter, Inc. (NYSE: BEC ), a leading ... and innovate complex biomedical tests,announces upcoming company presentations ... (Logo: http://www.newscom.com/cgi-bin/prnh/20031202/BECLOGO ), ... July 25, 2008., -- July 28 - ...
... Universities of Cambridge and Bristol have revealed that the ... the vacuum of a mass spectrometer. Their research ... Express . , The researchers were surprised to discover ... always been assumed that they would not survive once ...
Cached Biology Technology:Upcoming Beckman Coulter Investor Events Second Half of 2008 2Membrane complexes take flight 2
(Date:5/18/2013)... 18, 2013) Research presented today at Digestive Disease ... with findings about the impact of coffee on autoimmune ... While coffee consumption recently has been associated with reduced ... a few more cups of java each month also ... disease. Researchers at the Mayo Clinic, Rochester, MN, linked ...
(Date:5/18/2013)... 2013) Research presented at Digestive Disease Week ... through diet and lifestyle. , Individuals suffering ... muscle strength, fatigue and poor quality of life. ... in remission. A randomized, double blind, placebo-controlled study ... supplementation corresponded to significant relief of these symptoms. ...
(Date:5/18/2013)... 18, 2013) An increasing number of U.S. ... to resolve, according to research presented at Digestive ... targeting obesity, researchers at the Cleveland Clinic Children,s ... pattern of exhaled breath compared to their lean ... compound levels that can be correlated to potential ...
Breaking Biology News(10 mins):Coffee consumption associated with reduced risk of autoimmune liver disease 2Research examines new methods for managing digestive health 2Research examines new methods for managing digestive health 3New research identifies risks, interventions for children's GI health 2New research identifies risks, interventions for children's GI health 3
... A global collaborative has produced a first draft ... that will lead to insights in agriculture, medicine, conservation ... farm at the University of Illinois will now be ... had their genomes sequenced. Researchers will announce the achievement ...
... / - PDC Biotech GmbH (PDC) today announced the ... Verwaltungs AG (Munich) and Edmond de Rothschild Investment Partners (Paris) co-led ... used to complete preclinical work and the first clinical study of ... for the treatment of preterm labour and primary dysmenorrhea . ...
... 2009) -- A husband and wife team of American paleontologists has ... ago during the early Cretaceous of central Montana. The new ... of the Canadian Journal of Earth Sciences . Ankylosaurs are ... a plate-like armour with two sets of sharp spikes on each ...
Cached Biology News:First draft of the pig: Researchers sequence swine genome 2First draft of the pig: Researchers sequence swine genome 3PDC Biotech GmbH Announces Close of Series A Financing 2PDC Biotech GmbH Announces Close of Series A Financing 3New dinosaur species from Montana 2
TXNL2 Antibody...
Goat polyclonal to Glucose Dehydrogenase ( Abpromise for all tested applications). Antigen: Glucose Dehydrogenase (Bacillus species)...
Goat polyclonal to HNF4 ( Abpromise for all tested applications). Antigen: Synthetic peptide: RLSKTLVDMDMADY (Human) N terminal. Entrez GeneID: 3172 Swiss Protein ID: P41235...
ATGL Antibody Shipping Temperature: HOT Storage Temperature: -20C...
Biology Products: