Navigation Links
RT-PCR Primers for the Study of Apoptosis

O. Mineral oil (100 l) was added on each tube.

The PCR amplifications were cycled using the RoboCycler temperature cycler and the following parameters: 1 cycle of 94C for 5 minutes; 40 cycles of 94C for 1 minute, 60C for 2 minutes and 72C for 2 minutes; 1 cycle of 72C for 10 minutes. The reactions were stored at 6C. From each PCR amplification, 20 l was analyzed by electrophoresis on a 1.2% agarose gel in TAE buffer with 1 mg/ml of ethidium bromide for staining. The results were visualized using Stratagenes Eagle Eye II still video system.

Mouse ICE Family

Figure 1

A mouse macrophage cDNA library was used as template for the PCR amplifications with two sets of primers (Figure 1). One set, mouse ICE (a,b,g), amplifies the ICE gene and distinguishes the splice variants of ICE. These variants generate PCR products of different lengths: 610-bp PCR product for ICE-a, 520-bp PCR product for ICE-b, and 380-bp PCR product for ICE-g. In contrast, the ICE (all) primer set does not distinguish among the splice variants and generates a 470-bp product for all ICE variants.

Human ICE Family and Fas Antigen

Figure 2

To test human ICE-b , CPP32, ICE-Lap-3, and Fas antigen RT-PCR primers, five human cDNA libraries were used as templates (Figure 2 and Figure 3). Human genomic DNA was used as the genomic DNA control, and mouse and rat cDNA were tested as negative controls. Each amplification yielded
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Performance Comparisons of Commercial RT-PCR Systems
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: PCR Primers for the Study Apoptosis

(Date:5/25/2015)... 2015 The 2015 Market Research ... a professional and in-depth study on the current ... with a focus on the Chinese situation. ... research are Linde Industrial Gas, Basf, Air Products, ... Xinlong Group, Longsheng Group, Juhua Group, Wandali Gas, ...
(Date:5/22/2015)... plc (NYSE: ACT ) today confirmed that a ... Second Circuit has issued a ruling upholding a December ... distribution of NAMENDA ® (memantine HCl) immediate-release tablets. ... "While we are disappointed by the Court,s decision ... strong efforts to convey the significant benefits of NAMENDA ...
(Date:5/22/2015)... 22, 2015 Charm Sciences, Inc. ... of Agriculture (USDA), Grain Inspection, Packers and Stockyards ... to Charm Sciences to monitor aflatoxin in grains ... and ROSA FAST Aflatoxin Quantitative Test (solvent-based). , ... uses Water Extraction Technology to extract aflatoxin ...
(Date:5/22/2015)... Baylor Research Institute (BRI) at Dallas ... in Phoenix, AZ , today announce an ... for patients with a broad range of cancers. Aligning the ... trials and access to technology to drive the development of ... across Baylor Scott & White Health,s 49 hospitals ...
Breaking Biology Technology:Global Hydrogen Chloride Industry 2015 Trends and Analysis Available in a New Report at DeepResearchReports.com 2Global Hydrogen Chloride Industry 2015 Trends and Analysis Available in a New Report at DeepResearchReports.com 3Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 2Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 3Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 4Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 5Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 6Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 7Actavis Confirms Appeals Court Ruling Requiring Continued Distribution of NAMENDA IR 8USDA-GIPSA (FGIS) Awards 5 Year Contract for Aflatoxin Tests to Charm Sciences 2Precision Medicine Collaboration to Increase Options for Cancer Patients 2Precision Medicine Collaboration to Increase Options for Cancer Patients 3Precision Medicine Collaboration to Increase Options for Cancer Patients 4
... Million; GAAP EPS $0.34; ... Adjusted EPS $0.35, Fiscal Year 2007: Total Net Revenue Reaches $2.50 ... $1.27; 2007 Adjusted EPS ... leading specialty pharmaceutical,company, today reported fourth quarter and full year 2007 financial results,for its ...
... Cylex, Inc., today announced the,publication of an independent, ... of cellular immune response measured prior to kidney,transplantation ... was found to identify patients that may be ... kidney function in the first three,months following transplantation., ...
... supplier of,innovative services to healthcare providers, announced today ... its Cardiovascular Program.,VascularAware is designed to help people ... (PAD), which affects nearly 10 million adult Americans,according ... the VascularAware disease module extends the Company,s,mission of ...
Cached Biology Technology:Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 2Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 3Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 4Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 5Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 6Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 7Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 8Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 9Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 10Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 11Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 12Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 13Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 14Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 15Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 16Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 17Watson Pharmaceuticals Reports Fourth Quarter and Full Year 2007 Results; Provides 2008 Outlook 18New Independent Research Study Indicates That Cylex's ImmuKnow Test May Identify Kidney Transplant Patients at Risk for Early Acute Rejection 2New Independent Research Study Indicates That Cylex's ImmuKnow Test May Identify Kidney Transplant Patients at Risk for Early Acute Rejection 3HealthAware Introduces Its VascularAware Cardiovascular Disease Module 2
(Date:5/22/2015)... , May 22, 2015 According ... by Solution (2D, 3D, Thermal, Emotion, Forensic), by Software (Middleware, ... (Government and Utilities, Consumer and Home) - Global Forecast ... grow from $2.77 Billion in 2015 to $6.19 Billion ... Browse 79 market data Tables and   43 ...
(Date:5/20/2015)... 20, 2015 ... announced the addition of the  "5-year ... Recognition Market"  report to their offering.  ... service analyses trends in the iris ... commercial sectors, globally. Despite hardware pricing ...
(Date:5/19/2015)... -- Technology is rapidly advancing, outpacing enterprises, ability to secure ... and their management are soon to be rendered obsolete ... as those developed by the FIDO Alliance™. With the ... BYOD, COPE, IoT, and mobile payments, the interconnected online ... In response to the call for seamless, secure, ...
Breaking Biology News(10 mins):Facial Recognition Market Worth $6.19 Billion by 2020 2Facial Recognition Market Worth $6.19 Billion by 2020 3Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3
... The international research team led by Chinese Academy of ... and analysis of a diploid cotton-- Gossypium raimondii ... the study and genetic improvement of cotton quality and ... characteristics and evolutionary mechanism underlying cotton and its close ...
... Immediate Release August 28, 2012 (Toronto) Six ... (CAMH) have received prestigious NARSAD Young Investigator Grants from ... awards will fund research on biological factors underlying schizophrenia, ... stimulation and brain imaging techniques, which promise to lead ...
... An international team of scientists has discovered the oldest ... crustaceanspreserved in amber. The specimens, one fly and two ... Italy, are about 100 million years older than any ... are published today in the Proceedings of the ...
Cached Biology News:Chinese scientists successfully crack the genome of diploid cotton 26 NARSAD Young Investigator Grants awarded to CAMH 26 NARSAD Young Investigator Grants awarded to CAMH 3Scientists find oldest occurrence of arthropods preserved in amber 2Scientists find oldest occurrence of arthropods preserved in amber 3
... of UVP's Doc-It systems. When further analysis ... software onto your existing computer. Doc-It ... enhancement tools. Doc-It 1D Analysis Software provides ... quick and accurate electrophoresis analysis., Enhance-It ...
CQCS kit for first KR 4i , (first unit)....
... new generation dispenser combining the most versatile features ... of the existing Multidrop dispensers. , ,It ... from 500 nl up to 2500 l with ... is optimized for multiple plate formats (96, 384, ...
Multidrop Micro high speed 1 l bulk reagent dispenser rapidly, precisely and accurately dispenses microvolumes of 1-50 l into 384- and 96-well microplates....
Biology Products: